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Different biological effect of estrogen receptor-related receptor alpha in estrogen receptor-positive and -negative endometrial carcinoma
Gao, Min1,2; Sun, Peng-Ming1; Wang, Jian-Liu1; Li, Xiao-Ping1; Zhao, Chao1; Wei, Li-Hui1
关键词estrogen receptor-related receptor alpha estrogen receptor alpha endometrial carcinoma interaction
刊名MOLECULAR MEDICINE REPORTS
2008-11-01
DOI10.3892/mmr_00000050
1期:6页:917-924
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pathology
研究领域[WOS]Pathology
关键词[WOS]ORPHAN NUCLEAR RECEPTORS ; HUMAN LACTOFERRIN GENE ; RESPONSE ELEMENTS ; ERR-ALPHA ; TRANSCRIPTIONAL REGULATION ; BREAST-CANCER ; EXPRESSION ; COACTIVATOR ; BETA ; BINDING
英文摘要

Estrogen receptor-related receptor alpha (ERR alpha) has been identified as a nuclear transfactor closely related to estrogen receptor alpha (ER alpha). ERR alpha interferes with ER-mediated signaling pathways through competition with ERa for the common DNA sites and coregulators. Thus, it may participate in the tumorigenesis of estrogen-related cancers. To elucidate the roles of ERR alpha in endometrial carcinogenesis and the crosstalk between ER alpha and ERR alpha, endometrial carcinoma Ishikawa and HEC-IA cells were treated with different concentrations of 17ss-E(2), and/or the pure anti-estrogen drug ICI 182,780. Using semi-quantitative RT-PCR and Western blot analysis, we found that 17ss-E(2) down-regulated the expression of ERR alpha in ER-positive Ishikawa cells, while up-regulating the expression of ERR alpha in ER-negative HEC-IA cells. Down-regulation in Ishikawa cells was furthermore found to be largely abrogated by ICI 182,780. Additionally, we constructed endometrial carcinoma cell lines with overexpression of ERR alpha by stable transfection, renaming these Ishikawa/ERR alpha and HEC-IA/ERR alpha, respectively. To investigate the effect of ERRa overexpression on the biological behavior of the cells, MTT assay and flow cytometry analysis were performed in the constructed cell lines. In ER-positive Ishikawa cells, the overexpression of ERR alpha inhibited cell growth in the presence of 17ss-E(2) an inhibitory effect that might be due to a G(0)-G(1) cell cycle arrest. In contrast, overexpression of ERR alpha stimulated cell proliferation in ER-negative HEC-IA cells independently of 17ss-E(2). This accelerated action was associated with changes in cell cycle distribution. Our study demonstrates that, in addition to ER, ERR alpha seems to be an important regulator in endometrial carcinogenesis, playing different roles in estrogen-dependent and -independent endometrial carcinomas. ERR alpha might modulate the ER-mediated pathways via interference with ER alpha transcription in endometrial carcinoma cells.

语种英语
WOS记录号WOS:000261058300024
项目编号30371477
资助机构National Nature Science Foundation of China
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63161
专题北京大学第二临床医学院
北京大学第二临床医学院_妇科
作者单位1.Peking Univ, Peoples Hosp, Dept Obstet & Gynecol, Beijing 100044, Peoples R China
2.Peking Univ, Sch Oncol, Dept Gynecol, Beijing Canc Hosp & Inst, Beijing 100036, Peoples R China
推荐引用方式
GB/T 7714
Gao, Min,Sun, Peng-Ming,Wang, Jian-Liu,et al. Different biological effect of estrogen receptor-related receptor alpha in estrogen receptor-positive and -negative endometrial carcinoma[J]. MOLECULAR MEDICINE REPORTS,2008,1(6):917-924.
APA Gao, Min,Sun, Peng-Ming,Wang, Jian-Liu,Li, Xiao-Ping,Zhao, Chao,&Wei, Li-Hui.(2008).Different biological effect of estrogen receptor-related receptor alpha in estrogen receptor-positive and -negative endometrial carcinoma.MOLECULAR MEDICINE REPORTS,1(6),917-924.
MLA Gao, Min,et al."Different biological effect of estrogen receptor-related receptor alpha in estrogen receptor-positive and -negative endometrial carcinoma".MOLECULAR MEDICINE REPORTS 1.6(2008):917-924.
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