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学科主题临床医学
Comparison of commonly used retrograde tracers in rat spinal motor neurons
Yu, You-lai1; Li, Hai-yan2; Zhang, Pei-xun1; Yin, Xiao-feng1; Han, Na1; Kou, Yu-hui1; Jiang, Bao-guo1
关键词nerve regeneration tracing efficacy fluorescent tracers retrograde tracing femoral nerve motor neurons
刊名Neural Regeneration Research
2015-10-01
DOI10.4103/1673-5374.167772
10期:10页:1700-1705
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Neurosciences
资助者National Program on Key Basic Research Project (973 Program) ; Innovative Research Team by the Ministry of Education ; National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation of China ; National Program on Key Basic Research Project (973 Program) ; Innovative Research Team by the Ministry of Education ; National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation of China
研究领域[WOS]Cell Biology ; Neurosciences & Neurology
关键词[WOS]PERIPHERAL-NERVE INJURY ; FLUORESCENT TRACERS ; FAST-BLUE ; AFFERENT NEURONS ; FEMORAL NERVE ; FLUORO-RUBY ; REINNERVATION ; PATHWAYS ; REGENERATION ; ACCURACY
英文摘要

The purpose of this study was to investigate the effect of four fluorescent dyes, True Blue (TB), Fluoro-Gold (FG), Fluoro-Ruby (FR), and 1,1′-dioctadecy1-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI), in retrograde tracing of rat spinal motor neurons. We transected the muscle branch of the rat femoral nerve and applied each tracer to the proximal stump in single labeling experiments, or combinations of tracers (FG-Dif and TB-DiI) in double labeling experiments. In the single labeling experiments, significantly fewer labeled motor neurons were observed after FR labeling than after TB, FG, or DiI, 3 days after tracer application. By 1 week, there were no significant differences in the number of labeled neurons between the four groups. In the double-labeling experiment, the number of double-labeled neurons in the FG-DiI group was not significantly different from that in the TB-Dif group 1 week after tracer application. Our findings indicate that TB, FG, and DiI have similar labeling efficacies in the retrograde labeling of spinal motor neurons in the rat femoral nerve when used alone. Furthermore, combinations of Dif and TB or PG are similarly effective. Therefore, of the dyes studied, TB, PG and DiI, and combinations of DiI with TB or FG, are the most suitable for retrograde labeling studies of motor neurons in the rat femoral nerve.

语种英语
所属项目编号2014CB542200 ; IRT1201 ; 31271284 ; 31171150 ; 81171146 ; 30971526 ; 31040043 ; 31371210 ; 81372044 ; 31471144 ; 7142164
资助者National Program on Key Basic Research Project (973 Program) ; Innovative Research Team by the Ministry of Education ; National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation of China ; National Program on Key Basic Research Project (973 Program) ; Innovative Research Team by the Ministry of Education ; National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation of China
WOS记录号WOS:000364260700043
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63162
专题北京大学第二临床医学院
作者单位1.Peking Univ, Peoples Hosp, Dept Trauma & Orthoped, Beijing 100871, Peoples R China
2.Weifang Peoples Hosp, Dept Gastroenterol, Weifang, Shandong, Peoples R China
推荐引用方式
GB/T 7714
Yu, You-lai,Li, Hai-yan,Zhang, Pei-xun,et al. Comparison of commonly used retrograde tracers in rat spinal motor neurons[J]. Neural Regeneration Research,2015,10(10):1700-1705.
APA Yu, You-lai.,Li, Hai-yan.,Zhang, Pei-xun.,Yin, Xiao-feng.,Han, Na.,...&Jiang, Bao-guo.(2015).Comparison of commonly used retrograde tracers in rat spinal motor neurons.Neural Regeneration Research,10(10),1700-1705.
MLA Yu, You-lai,et al."Comparison of commonly used retrograde tracers in rat spinal motor neurons".Neural Regeneration Research 10.10(2015):1700-1705.
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