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学科主题: 基础医学
题名:
Upregulation of Flk-1 by bFGF via the ERK pathway is essential for VEGF-mediated promotion of neural stem cell proliferation
作者: Xiao, Zhifeng; Kong, Yaxian; Yang, Shufa; Li, Meiyu; Wen, Jinhua; Li, Lingsong
关键词: neural stem cells ; vascular endothelial growth factor ; fibroblast growth factor ; embryonic stem cells
刊名: CELL RESEARCH
发表日期: 2007
DOI: 10.1038/sj.cr.7310126
卷: 17, 期:1, 页:73-79
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: ENDOTHELIAL GROWTH-FACTOR ; DEVELOPING CEREBRAL-CORTEX ; CENTRAL-NERVOUS-SYSTEM ; IN-VITRO ; PROGENITOR CELLS ; PRECURSOR CELLS ; NEUROGENESIS ; RECEPTORS ; EXPRESSION ; LETHALITY
英文摘要:

Neural stem cells (NSCs) constitute the cellular basis for embryonic brain development and neurogenesis. The process is regulated by NSC niche including neighbor cells such as vascular and glial cells. Since both vascular and glial cells secrete vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), we assessed the effect of VEGF and bFGF on NSC proliferation using nearly homogeneous NSCs that were differentiated from mouse embryonic stem cells. VEGF alone did not have any significant effect. When bFGF was added, however, VEGF stimulated NSC proliferation in a dose-dependent manner, and this stimulation was inhibited by ZM323881, a VEGF receptor (Flk-1)-specific inhibitor. Interestingly, ZM323881 also inhibited cell proliferation in the absence of exogenous VEGF, suggesting that VEGF autocrine plays a role in the proliferation of NSCs. The stimulatory effect of VEGF on NSC proliferation depends on bFGF, which is likely due to the fact that expression of Flk-1 was upregulated by bFGF via phosphorylation of ERK1/2. Collectively, this study may provide insight into the mechanisms by which microenviromnental niche signals regulate NSCs.

语种: 英语
WOS记录号: WOS:000244352700010
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63165
Appears in Collections:基础医学院_北京大学干细胞研究中心_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Beijing 100871, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Beijing 100871, Peoples R China

Recommended Citation:
Xiao, Zhifeng,Kong, Yaxian,Yang, Shufa,et al. Upregulation of Flk-1 by bFGF via the ERK pathway is essential for VEGF-mediated promotion of neural stem cell proliferation[J]. CELL RESEARCH,2007,17(1):73-79.
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