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A functional promoter region of the CKLFSF2 gene is located in the last intron/exon region of the upstream CKLFSF1 gene
Xu, MX; Yang, SH; Gao, YH; Shi, S; Ma, DL
关键词promoter CKLFSF1 CKLFSF2
刊名INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
2005-06-01
DOI10.1016/j.biocel.2005.01.004
37期:6页:1296-1307
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]RNA-POLYMERASE-II ; XENOPUS-LAEVIS ; MESSENGER-RNA ; IDENTIFICATION ; TRANSCRIPTION ; SEQUENCE ; ELEMENTS ; PROTEIN ; TISSUE ; INTRON-9
英文摘要

The genes for CKLFSF1 (chemokine-like factor super family member 1) and CKLFSF2 (chemokine-like factor super family member 2) are very closely linked, within 312 bp of each other. Here, we present evidence that the last intron/exon region of the CKLFSF1 gene contains a novel eukaryotic promoter capable of directing the expression of the downstream gene, CKLFSF2. We identified two segments of the upstream region of the CKLFSF2 gene, 2146 bp (-2134/+12, relative to ATG + 1) and 1483 bp (-2134/-652), that were capable of efficiently driving expression of a linked reporter gene upon transient transfection into several kinds of cell lines. The 1483 bp segment exhibited more than a two-fold increase in luciferase activity relative to the 2146 bp segment. By analyzing 5′-deletion mutants of the 1483 bp segment, we identified a 195 bp segment (-846/-625) located in the last intron/exon region of the CKLFSF1 gene that was critical for promoter activity. DNA decoy experiments revealed that a 122 bp (-846/-725) fragment markedly inhibited CKLFSF2 mRNA transcription. Furthermore. we found that the putative promoter region of the CKLFSF2 gene is separated from the transcription start site by about 500 bp. Accumulating reports suggest that introns have many functions, including the modulation of regulation and structure. This work provides evidence that a eukaryotic gene promoter sequence from one gene located in an intron/exon of another. (c) 2005 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000228373300015
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63166
专题基础医学院_免疫学系
作者单位1.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China
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Xu, MX,Yang, SH,Gao, YH,et al. A functional promoter region of the CKLFSF2 gene is located in the last intron/exon region of the upstream CKLFSF1 gene[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2005,37(6):1296-1307.
APA Xu, MX,Yang, SH,Gao, YH,Shi, S,&Ma, DL.(2005).A functional promoter region of the CKLFSF2 gene is located in the last intron/exon region of the upstream CKLFSF1 gene.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,37(6),1296-1307.
MLA Xu, MX,et al."A functional promoter region of the CKLFSF2 gene is located in the last intron/exon region of the upstream CKLFSF1 gene".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 37.6(2005):1296-1307.
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