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On-Chip Cryopreservation: A Novel Method for Ultra-Rapid Cryoprotectant-Free Cryopreservation of Small Amounts of Human Spermatozoa
Zou, Yujie1; Yin, Tailang1,2; Chen, Shijing3; Yang, Jing1; Huang, Weihua3
刊名PLOS ONE
2013-04-30
DOI10.1371/journal.pone.0061593
8期:4
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]CHRONICALLY INFECTED WOMEN ; LIQUID-NITROGEN SAMPLES ; SPENT CULTURE-MEDIA ; SPERM DNA INTEGRITY ; VITRIFICATION ; ZONA ; TECHNOLOGY ; OOCYTES ; EMBRYOS ; CYCLES
英文摘要

Cryopreservation of human spermatozoa free from cryoprotectant can avoid toxicity caused by highly concentrated cryoprotectant and a series of specific carriers have been previously explored, except for PDMS chip. Our study is aimed at exploring a novel device for ultra-rapid cryopreservation of small numbers of spermatozoa without cryoprotectant based on polydimethylsiloxane (PDMS) chips. Spermatozoa from 25 healthy men were involved in this study, comparing on-chip cryopreservation with different micro-channel height (group A: 10 mu m height, group B: 50 mu m height, group C: 100 mu m height) and conventional freezing (group D) in liquid nitrogen for 72 h. The viability, motility, DNA integrity by comet assay and acrosome integrity by fluorescein isothiocyanate-conjugated peanut agglutinin (FITC-PNA) staining of frozen-thawed spermatozoa of each group were compared. The motility and viability of post-thawed spermatozoa was significantly decreased than that of pre-freezing spermatozoa. There was no difference of viability and motility of frozen-thawed spermatozoa between group A and D, while viability and motility of group B and C decreased compared to group A. Comet assay showed that no matter for group A or D, there was no difference of CR, TL, TD and OTM between pre-frozen and post-thawed spermatozoa. There was no difference of CR, TL, TD and OTM of post-thawed spermatozoa between group A and group D neither, while spermatozoa DNA damage was more serious in group B and group C with increasing height of micro-channel compared with group A. The proportion of intact acrosome of post-thawed spermatozoa in group A was the highest when compared with group B and group C, though similar to that of group D. In conclusion, PDMS chip with 10 mu m height micro-channel is ideal for ultra-rapid cryopreservation of small quantity of spermatozoa without cryoprotectant.

语种英语
WOS记录号WOS:000319077300011
项目编号30973196 ; 20975077 ; 31070995 ; 20921062 ; NCET-10-0611
资助机构National Natural Science Foundation of China ; Science Fund for Creative Research Groups ; Program for New Century Excellent Talents in University
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63176
专题北京大学第三临床医学院_生殖医学中心
作者单位1.Wuhan Univ, Renmin Hosp, Reprod Med Ctr, Wuhan 430072, Peoples R China
2.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100871, Peoples R China
3.Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Peoples R China
推荐引用方式
GB/T 7714
Zou, Yujie,Yin, Tailang,Chen, Shijing,et al. On-Chip Cryopreservation: A Novel Method for Ultra-Rapid Cryoprotectant-Free Cryopreservation of Small Amounts of Human Spermatozoa[J]. PLOS ONE,2013,8(4).
APA Zou, Yujie,Yin, Tailang,Chen, Shijing,Yang, Jing,&Huang, Weihua.(2013).On-Chip Cryopreservation: A Novel Method for Ultra-Rapid Cryoprotectant-Free Cryopreservation of Small Amounts of Human Spermatozoa.PLOS ONE,8(4).
MLA Zou, Yujie,et al."On-Chip Cryopreservation: A Novel Method for Ultra-Rapid Cryoprotectant-Free Cryopreservation of Small Amounts of Human Spermatozoa".PLOS ONE 8.4(2013).
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