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学科主题: 临床医学
题名:
On-Chip Cryopreservation: A Novel Method for Ultra-Rapid Cryoprotectant-Free Cryopreservation of Small Amounts of Human Spermatozoa
作者: Zou, Yujie1; Yin, Tailang1,2; Chen, Shijing3; Yang, Jing1; Huang, Weihua3
刊名: PLOS ONE
发表日期: 2013-04-30
DOI: 10.1371/journal.pone.0061593
卷: 8, 期:4
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: CHRONICALLY INFECTED WOMEN ; LIQUID-NITROGEN SAMPLES ; SPENT CULTURE-MEDIA ; SPERM DNA INTEGRITY ; VITRIFICATION ; ZONA ; TECHNOLOGY ; OOCYTES ; EMBRYOS ; CYCLES
英文摘要:

Cryopreservation of human spermatozoa free from cryoprotectant can avoid toxicity caused by highly concentrated cryoprotectant and a series of specific carriers have been previously explored, except for PDMS chip. Our study is aimed at exploring a novel device for ultra-rapid cryopreservation of small numbers of spermatozoa without cryoprotectant based on polydimethylsiloxane (PDMS) chips. Spermatozoa from 25 healthy men were involved in this study, comparing on-chip cryopreservation with different micro-channel height (group A: 10 mu m height, group B: 50 mu m height, group C: 100 mu m height) and conventional freezing (group D) in liquid nitrogen for 72 h. The viability, motility, DNA integrity by comet assay and acrosome integrity by fluorescein isothiocyanate-conjugated peanut agglutinin (FITC-PNA) staining of frozen-thawed spermatozoa of each group were compared. The motility and viability of post-thawed spermatozoa was significantly decreased than that of pre-freezing spermatozoa. There was no difference of viability and motility of frozen-thawed spermatozoa between group A and D, while viability and motility of group B and C decreased compared to group A. Comet assay showed that no matter for group A or D, there was no difference of CR, TL, TD and OTM between pre-frozen and post-thawed spermatozoa. There was no difference of CR, TL, TD and OTM of post-thawed spermatozoa between group A and group D neither, while spermatozoa DNA damage was more serious in group B and group C with increasing height of micro-channel compared with group A. The proportion of intact acrosome of post-thawed spermatozoa in group A was the highest when compared with group B and group C, though similar to that of group D. In conclusion, PDMS chip with 10 mu m height micro-channel is ideal for ultra-rapid cryopreservation of small quantity of spermatozoa without cryoprotectant.

语种: 英语
所属项目编号: 30973196 ; 20975077 ; 31070995 ; 20921062 ; NCET-10-0611
项目资助者: National Natural Science Foundation of China ; Science Fund for Creative Research Groups ; Program for New Century Excellent Talents in University
WOS记录号: WOS:000319077300011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63176
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Wuhan Univ, Renmin Hosp, Reprod Med Ctr, Wuhan 430072, Peoples R China
2.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100871, Peoples R China
3.Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Peoples R China

Recommended Citation:
Zou, Yujie,Yin, Tailang,Chen, Shijing,et al. On-Chip Cryopreservation: A Novel Method for Ultra-Rapid Cryoprotectant-Free Cryopreservation of Small Amounts of Human Spermatozoa[J]. PLOS ONE,2013,8(4).
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