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Calcitonin gene-related peptide induces proliferation and monocyte chemoattractant protein-1 expression via extracellular signal-regulated kinase activation in rat osteoblasts
Han Na; Zhang Dian-ying; Wang Tian-bing; Zhang Pei-xun; Jiang Bao-guo
关键词calcitonin gene-related peptide monocyte chemoattractant protein-1 rat primary osteoblasts extracellular signal-regulated kinase
刊名CHINESE MEDICAL JOURNAL
2010-07-05
DOI10.3760/cma.j.issn.0366-6999.2010.13.025
123期:13页:1748-1753
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]BONE METABOLISM ; IFN-GAMMA ; CELLS ; AMYLIN ; CGRP ; FRACTURE ; RECEPTORS ; MCP-1 ; MICE
英文摘要

Background Calcitonin gene-related peptide (CGRP), a sensory neuropeptide, affects osteoblast proliferation and bone formation. However, the mechanisms are not fully understood. Monocyte chemoattractant protein-1 (MCP-1) is a chemokine that stimulates the migration of monocytes and plays important roles in regulating bone remolding during fracture repair. In this study, we investigated the effects of CGRP on proliferation and MCP-1 expression in cultured rat osteoblasts.

Methods Primary rat osteoblasts were isolated from fetal rats calvariae. Cells were exposed to gradient concentrations (10(-9) to 10(-7) mol/L) of CGRP. Protein and mRNA levels of MCP-1 were quantified by Western blotting and semiquantitative reverse transcription-polymerase chain reaction, respectively. The protein level of MCP-1 was investigated and compared in cell culture media by enzyme linked innmunosorbent assay (ELISA). Phospho-extracellular signal-regulated kinase (ERK) expression was detected by Western blotting. Cell proliferative activity was measured by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MU) and BrdU assay. The effects of MAPK/ERK kinase (MEK)-inhibitor U0126 on CGRP-induced MCP-1 expression in primary rat osteoblasts were examined.

Results CGRP effectively enhanced primary rat osteoblast proliferation and led to significant increases in the expression of MCP-1 mRNA and protein in time- and dose-dependent manners. CGRP activated the ERK pathway. Pretreatment of cultured rat osteoblasts with MEK inhibitor U0126 resulted in dose-dependent inhibitions of CGRP-induced MCP-1 mRNA and protein levels. Thus, CGRP promoted cell proliferation and stimulated MCP-1 expression in cultured rat osteoblasts.

Conclusion These studies document novel links between CGRP and MCP-1 and illuminate the effects of CGRP in regulating bone remodeling. Chin Med J 2010;123(13):1748-1753

语种英语
WOS记录号WOS:000280085300025
项目编号2007BA104B00 ; 2007-2011
资助机构Chinese Government ; Capital Medical Development Science Research Program
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63178
专题北京大学第二临床医学院
北京大学第二临床医学院_创伤骨科
北京大学第二临床医学院_党委院长办公室
作者单位Peking Univ, Peoples Hosp, Dept Orthopaed & Trauma, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Han Na,Zhang Dian-ying,Wang Tian-bing,et al. Calcitonin gene-related peptide induces proliferation and monocyte chemoattractant protein-1 expression via extracellular signal-regulated kinase activation in rat osteoblasts[J]. CHINESE MEDICAL JOURNAL,2010,123(13):1748-1753.
APA Han Na,Zhang Dian-ying,Wang Tian-bing,Zhang Pei-xun,&Jiang Bao-guo.(2010).Calcitonin gene-related peptide induces proliferation and monocyte chemoattractant protein-1 expression via extracellular signal-regulated kinase activation in rat osteoblasts.CHINESE MEDICAL JOURNAL,123(13),1748-1753.
MLA Han Na,et al."Calcitonin gene-related peptide induces proliferation and monocyte chemoattractant protein-1 expression via extracellular signal-regulated kinase activation in rat osteoblasts".CHINESE MEDICAL JOURNAL 123.13(2010):1748-1753.
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