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学科主题: 临床医学
题名:
Calcitonin gene-related peptide induces proliferation and monocyte chemoattractant protein-1 expression via extracellular signal-regulated kinase activation in rat osteoblasts
作者: Han Na; Zhang Dian-ying; Wang Tian-bing; Zhang Pei-xun; Jiang Bao-guo
关键词: calcitonin gene-related peptide ; monocyte chemoattractant protein-1 ; rat primary osteoblasts ; extracellular signal-regulated kinase
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2010-07-05
DOI: 10.3760/cma.j.issn.0366-6999.2010.13.025
卷: 123, 期:13, 页:1748-1753
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: BONE METABOLISM ; IFN-GAMMA ; CELLS ; AMYLIN ; CGRP ; FRACTURE ; RECEPTORS ; MCP-1 ; MICE
英文摘要:

Background Calcitonin gene-related peptide (CGRP), a sensory neuropeptide, affects osteoblast proliferation and bone formation. However, the mechanisms are not fully understood. Monocyte chemoattractant protein-1 (MCP-1) is a chemokine that stimulates the migration of monocytes and plays important roles in regulating bone remolding during fracture repair. In this study, we investigated the effects of CGRP on proliferation and MCP-1 expression in cultured rat osteoblasts.

Methods Primary rat osteoblasts were isolated from fetal rats calvariae. Cells were exposed to gradient concentrations (10(-9) to 10(-7) mol/L) of CGRP. Protein and mRNA levels of MCP-1 were quantified by Western blotting and semiquantitative reverse transcription-polymerase chain reaction, respectively. The protein level of MCP-1 was investigated and compared in cell culture media by enzyme linked innmunosorbent assay (ELISA). Phospho-extracellular signal-regulated kinase (ERK) expression was detected by Western blotting. Cell proliferative activity was measured by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MU) and BrdU assay. The effects of MAPK/ERK kinase (MEK)-inhibitor U0126 on CGRP-induced MCP-1 expression in primary rat osteoblasts were examined.

Results CGRP effectively enhanced primary rat osteoblast proliferation and led to significant increases in the expression of MCP-1 mRNA and protein in time- and dose-dependent manners. CGRP activated the ERK pathway. Pretreatment of cultured rat osteoblasts with MEK inhibitor U0126 resulted in dose-dependent inhibitions of CGRP-induced MCP-1 mRNA and protein levels. Thus, CGRP promoted cell proliferation and stimulated MCP-1 expression in cultured rat osteoblasts.

Conclusion These studies document novel links between CGRP and MCP-1 and illuminate the effects of CGRP in regulating bone remodeling. Chin Med J 2010;123(13):1748-1753

语种: 英语
所属项目编号: 2007BA104B00 ; 2007-2011
项目资助者: Chinese Government ; Capital Medical Development Science Research Program
WOS记录号: WOS:000280085300025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63178
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Dept Orthopaed & Trauma, Beijing 100044, Peoples R China

Recommended Citation:
Han Na,Zhang Dian-ying,Wang Tian-bing,et al. Calcitonin gene-related peptide induces proliferation and monocyte chemoattractant protein-1 expression via extracellular signal-regulated kinase activation in rat osteoblasts[J]. CHINESE MEDICAL JOURNAL,2010,123(13):1748-1753.
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