IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所
学科主题临床医学
Polycaprolactone electrospun mesh conjugated with an MSC affinity peptide for MSC homing in vivo
Shao, Zhenxing1; Zhang, Xin1; Pi, Yanbin1; Wang, Xiaokun3; Jia, Zhuqing2; Zhu, Jingxian1; Dai, Linghui1; Chen, Wenqing1; Yin, Ling3; Chen, Haifeng3; Zhou, Chunyan2; Ao, Yingfang1
关键词Phage display Tissue engineering (TE) MSC-homing Peptide-modified polycaprolactone electrospun mesh
刊名BIOMATERIALS
2012-04-01
DOI10.1016/j.biomaterials.2012.01.033
33期:12页:3375-3387
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]MESENCHYMAL STEM-CELLS ; AUTOLOGOUS CHONDROCYTE IMPLANTATION ; THERAPY POSITION STATEMENT ; PHAGE DISPLAY ; INTERNATIONAL-SOCIETY ; RANDOMIZED-TRIAL ; STROMAL CELLS ; MICROFRACTURE ; BIOMATERIALS ; REGENERATION
英文摘要

Mesenchymal stem cell (MSC) is a promising cell source candidate in tissue engineering (TE) and regenerative medicine. However, the inability to target MSCs in tissues of interest with high efficiency and engraftment has become a significant barrier for MSC-based therapies. The mobilization and transfer of MSCs to defective/damaged sites in tissues or organs in vivo with high efficacy and efficiency has been a major concern. In the present study, we identified a peptide sequence (E7) with seven amino acids through phage display technology, which has a high specific affinity to bone marrow-derived MSCs. Subsequent analysis suggested that the peptide could efficiently interact specifically with MSCs without any species specificity. Thereafter, E7 was covalently conjugated onto polycaprolactone (PCL) electrospun meshes to construct an "MSC-homing device" for the recruitment of MSCs both in vitro and in vivo. The E7-conjugated PCL electrospun meshes were implanted into a cartilage defect site of rat knee joints, combined with a microfracture procedure to mobilize the endogenous MSCs. After 7 d of implantation, immunofluorescence staining showed that the cells grown into the E7-conjugated PCL electrospun meshes yielded a high positive rate for specific MSC surface markers (CD44, CD90, and CD105) compared with those in arginine-glycine-aspartic acid (RGD)-conjugated PCL electrospun meshes (63.67% vs. 3.03%; 59.37% vs. 2.98%; and 61.45% vs. 3.82%, respectively). Furthermore, the percentage of CD68 positive cells in the E7-conjugated PCL electrospun meshes was much lower than that in the RGD-conjugated PCL electrospun meshes (5.57% vs. 53.43%). This result indicates that E7-conjugated PCL electrospun meshes absorb much less inflammatory cells in vivo than RGD-conjugated PCL electrospun meshes. The results of the present study suggest that the identified E7 peptide sequence has a high specific affinity to MSCs. Covalently conjugating this peptide on the synthetic PCL mesh significantly enhanced the MSC recruitment of PCL in vivo. This method provides a wide range of potential applications in TE. (C) 2012 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000301886300001
项目编号81071474 ; 2011AA030102 ; 2012CB933903 ; 20090001120117 ; 20110001130001
资助机构National Natural Science Foundation of China (NSFC) ; Ministry of Science and Technology of China ; Research Fund for the Doctoral Program of Higher Education of China ; Specialized Research Fund for the Doctoral Program of Higher Education
引用统计
被引频次:58[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63187
专题北京大学第三临床医学院_运动医学研究所
北京大学基础医学院
北京大学第一临床医学院_妇产科
北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
3.Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Shao, Zhenxing,Zhang, Xin,Pi, Yanbin,et al. Polycaprolactone electrospun mesh conjugated with an MSC affinity peptide for MSC homing in vivo[J]. BIOMATERIALS,2012,33(12):3375-3387.
APA Shao, Zhenxing.,Zhang, Xin.,Pi, Yanbin.,Wang, Xiaokun.,Jia, Zhuqing.,...&Ao, Yingfang.(2012).Polycaprolactone electrospun mesh conjugated with an MSC affinity peptide for MSC homing in vivo.BIOMATERIALS,33(12),3375-3387.
MLA Shao, Zhenxing,et al."Polycaprolactone electrospun mesh conjugated with an MSC affinity peptide for MSC homing in vivo".BIOMATERIALS 33.12(2012):3375-3387.
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