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IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所  > 期刊论文
学科主题: 临床医学
题名:
Polycaprolactone electrospun mesh conjugated with an MSC affinity peptide for MSC homing in vivo
作者: Shao, Zhenxing1; Zhang, Xin1; Pi, Yanbin1; Wang, Xiaokun3; Jia, Zhuqing2; Zhu, Jingxian1; Dai, Linghui1; Chen, Wenqing1; Yin, Ling3; Chen, Haifeng3; Zhou, Chunyan2; Ao, Yingfang1
关键词: Phage display ; Tissue engineering (TE) ; MSC-homing ; Peptide-modified polycaprolactone ; electrospun mesh
刊名: BIOMATERIALS
发表日期: 2012-04-01
DOI: 10.1016/j.biomaterials.2012.01.033
卷: 33, 期:12, 页:3375-3387
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: MESENCHYMAL STEM-CELLS ; AUTOLOGOUS CHONDROCYTE IMPLANTATION ; THERAPY POSITION STATEMENT ; PHAGE DISPLAY ; INTERNATIONAL-SOCIETY ; RANDOMIZED-TRIAL ; STROMAL CELLS ; MICROFRACTURE ; BIOMATERIALS ; REGENERATION
英文摘要:

Mesenchymal stem cell (MSC) is a promising cell source candidate in tissue engineering (TE) and regenerative medicine. However, the inability to target MSCs in tissues of interest with high efficiency and engraftment has become a significant barrier for MSC-based therapies. The mobilization and transfer of MSCs to defective/damaged sites in tissues or organs in vivo with high efficacy and efficiency has been a major concern. In the present study, we identified a peptide sequence (E7) with seven amino acids through phage display technology, which has a high specific affinity to bone marrow-derived MSCs. Subsequent analysis suggested that the peptide could efficiently interact specifically with MSCs without any species specificity. Thereafter, E7 was covalently conjugated onto polycaprolactone (PCL) electrospun meshes to construct an "MSC-homing device" for the recruitment of MSCs both in vitro and in vivo. The E7-conjugated PCL electrospun meshes were implanted into a cartilage defect site of rat knee joints, combined with a microfracture procedure to mobilize the endogenous MSCs. After 7 d of implantation, immunofluorescence staining showed that the cells grown into the E7-conjugated PCL electrospun meshes yielded a high positive rate for specific MSC surface markers (CD44, CD90, and CD105) compared with those in arginine-glycine-aspartic acid (RGD)-conjugated PCL electrospun meshes (63.67% vs. 3.03%; 59.37% vs. 2.98%; and 61.45% vs. 3.82%, respectively). Furthermore, the percentage of CD68 positive cells in the E7-conjugated PCL electrospun meshes was much lower than that in the RGD-conjugated PCL electrospun meshes (5.57% vs. 53.43%). This result indicates that E7-conjugated PCL electrospun meshes absorb much less inflammatory cells in vivo than RGD-conjugated PCL electrospun meshes. The results of the present study suggest that the identified E7 peptide sequence has a high specific affinity to MSCs. Covalently conjugating this peptide on the synthetic PCL mesh significantly enhanced the MSC recruitment of PCL in vivo. This method provides a wide range of potential applications in TE. (C) 2012 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 81071474 ; 2011AA030102 ; 2012CB933903 ; 20090001120117 ; 20110001130001
项目资助者: National Natural Science Foundation of China (NSFC) ; Ministry of Science and Technology of China ; Research Fund for the Doctoral Program of Higher Education of China ; Specialized Research Fund for the Doctoral Program of Higher Education
WOS记录号: WOS:000301886300001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63187
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
3.Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China

Recommended Citation:
Shao, Zhenxing,Zhang, Xin,Pi, Yanbin,et al. Polycaprolactone electrospun mesh conjugated with an MSC affinity peptide for MSC homing in vivo[J]. BIOMATERIALS,2012,33(12):3375-3387.
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