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学科主题: 基础医学
题名:
A ribosome-associating factor chaperones tail-anchored membrane proteins
作者: Mariappan, Malaiyalam1; Li, Xingzhe1,2; Stefanovic, Sandra1; Sharma, Ajay1; Mateja, Agnieszka3; Keenan, Robert J.3; Hegde, Ramanujan S.1
刊名: NATURE
发表日期: 2010-08-26
DOI: 10.1038/nature09296
卷: 466, 期:7310, 页:1120-U138
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: ENDOPLASMIC-RETICULUM ; ER MEMBRANE ; INSERTION ; GET3 ; COMPLEX ; RECOGNITION ; BINDING ; IDENTIFICATION ; TRANSLATION ; TERMINATION
英文摘要:

Hundreds of proteins are inserted post-translationally into the endoplasmic reticulum (ER) membrane by a single carboxyterminal transmembrane domain (TMD)(1). During targeting through the cytosol, the hydrophobic TMD of these tail-anchored (TA) proteins requires constant chaperoning to prevent aggregation or inappropriate interactions. A central component of this targeting system is TRC40, a conserved cytosolic factor that recognizes the TMD of TA proteins and delivers them to the ER for insertion(2-4). The mechanism that permits TRC40 to find and capture its TA protein cargos effectively in a highly crowded cytosol is unknown. Here we identify a conserved three-protein complex composed of Bat3, TRC35 and Ubl4A that facilitates TA protein capture by TRC40. This Bat3 complex is recruited to ribosomes synthesizing membrane proteins, interacts with the TMDs of newly released TA proteins, and transfers them to TRC40 for targeting. Depletion of the Bat3 complex allows non-TRC40 factors to compete for TA proteins, explaining their mislocalization in the analogous yeast deletion strains(5-7). Thus, the Bat3 complex acts as a TMD-selective chaperone that effectively channels TA proteins to the TRC40 insertion pathway.

语种: 英语
项目资助者: National Institutes of Health ; Edward Mallinckrodt Jr Foundation
WOS记录号: WOS:000281203600043
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63192
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Univ Chicago, Dept Biochem & Mol Biol, Chicago, IL 60637 USA
3.NICHHD, Cell Biol & Metab Program, NIH, Bethesda, MD 20892 USA

Recommended Citation:
Mariappan, Malaiyalam,Li, Xingzhe,Stefanovic, Sandra,et al. A ribosome-associating factor chaperones tail-anchored membrane proteins[J]. NATURE,2010,466(7310):1120-U138.
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