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A ribosome-associating factor chaperones tail-anchored membrane proteins
Mariappan, Malaiyalam1; Li, Xingzhe1,2; Stefanovic, Sandra1; Sharma, Ajay1; Mateja, Agnieszka3; Keenan, Robert J.3; Hegde, Ramanujan S.1
刊名NATURE
2010-08-26
DOI10.1038/nature09296
466期:7310页:1120-U138
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]ENDOPLASMIC-RETICULUM ; ER MEMBRANE ; INSERTION ; GET3 ; COMPLEX ; RECOGNITION ; BINDING ; IDENTIFICATION ; TRANSLATION ; TERMINATION
英文摘要

Hundreds of proteins are inserted post-translationally into the endoplasmic reticulum (ER) membrane by a single carboxyterminal transmembrane domain (TMD)(1). During targeting through the cytosol, the hydrophobic TMD of these tail-anchored (TA) proteins requires constant chaperoning to prevent aggregation or inappropriate interactions. A central component of this targeting system is TRC40, a conserved cytosolic factor that recognizes the TMD of TA proteins and delivers them to the ER for insertion(2-4). The mechanism that permits TRC40 to find and capture its TA protein cargos effectively in a highly crowded cytosol is unknown. Here we identify a conserved three-protein complex composed of Bat3, TRC35 and Ubl4A that facilitates TA protein capture by TRC40. This Bat3 complex is recruited to ribosomes synthesizing membrane proteins, interacts with the TMDs of newly released TA proteins, and transfers them to TRC40 for targeting. Depletion of the Bat3 complex allows non-TRC40 factors to compete for TA proteins, explaining their mislocalization in the analogous yeast deletion strains(5-7). Thus, the Bat3 complex acts as a TMD-selective chaperone that effectively channels TA proteins to the TRC40 insertion pathway.

语种英语
WOS记录号WOS:000281203600043
资助机构National Institutes of Health ; Edward Mallinckrodt Jr Foundation
引用统计
被引频次:132[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63192
专题北京大学基础医学院
作者单位1.Peking Univ, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Univ Chicago, Dept Biochem & Mol Biol, Chicago, IL 60637 USA
3.NICHHD, Cell Biol & Metab Program, NIH, Bethesda, MD 20892 USA
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Mariappan, Malaiyalam,Li, Xingzhe,Stefanovic, Sandra,et al. A ribosome-associating factor chaperones tail-anchored membrane proteins[J]. NATURE,2010,466(7310):1120-U138.
APA Mariappan, Malaiyalam.,Li, Xingzhe.,Stefanovic, Sandra.,Sharma, Ajay.,Mateja, Agnieszka.,...&Hegde, Ramanujan S..(2010).A ribosome-associating factor chaperones tail-anchored membrane proteins.NATURE,466(7310),1120-U138.
MLA Mariappan, Malaiyalam,et al."A ribosome-associating factor chaperones tail-anchored membrane proteins".NATURE 466.7310(2010):1120-U138.
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