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学科主题: 临床医学
题名:
Use of radioiodinated peptide Arg-Arg-Leu targeted to neovascularization as well as tumor cells in molecular tumor imaging
作者: Lu, Xia1,2,3; Yan, Ping1; Wang, Rong-fu1; Liu, Meng1; Yu, Ming-ming4; Zhang, Chun-li1
关键词: Iodine-131 ; Peptide Arg-Arg-Leu (tRRL) ; Uptake ability ; Molecular tumor imaging
刊名: CHINESE JOURNAL OF CANCER RESEARCH
发表日期: 2012-03-01
DOI: 10.1007/s11670-012-0052-8
卷: 24, 期:1, 页:52-59
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: PHAGE DISPLAY ; ANGIOGENESIS ; THERAPY ; GROWTH ; CANCER ; SCINTIGRAPHY ; VASCULATURE ; METASTASIS ; DISEASES
英文摘要:

To explore a tumor peptide imaging agent Arginine-Arginine-Leucine (Tyr-Cys-Gly-Gly-Arg-Arg-Leu-Gly-Gly-Cys, tripeptide RRL [tRRL]) that targeted to tumor cells and tumor-derived endothelial cells (TDECs) and primarily investigate the possible relationship between tRRL and vascular endothelial growth factor receptor 2 (VEGFR-2).

The tRRL sequence motif was identified as a tumor molecular marker specifically binding to TDECs. Tyrosine was conjugated to the amino terminal of RRL (Cys-Gly-Gly-Arg-Arg-Leu-Gly-Gly-Cys) for labeling with radionuclide iodine-131 (I-131-tRRL). The uptake ability and molecular binding of tRRL to tumor cells and angiogenic endothelium were studied using flow cytometry and radioactivity counter in vitro. Whether VEGFR-2 is the binging site of tRRL was investigated. Biodistribution and single-photon emission computed tomography (SPECT) imaging of I-131-tRRL were used to evaluate the effectiveness of this new imaging agent to visualize varied tumor xenografts in nude mice.

In vitro cellular uptake experiments revealed that tRRL could not only adhere to tumor angiogenic endothelial cells but also largely accumulate in malignant tumor cells. VEGFR-2, which is highly expressed on TDECs, was probably not the solely binding ligand for tRRL targeted to tumor angiogenic endothelium. I-131-tRRL mainly accumulated in tumors in vivo, not other organs at 24 h after injection. SPECT imaging with I-131-tRRL clearly visualized tumors in nude mice, especially at 24 h.

Radioiodinated tRRL offers a noninvasive nuclear imaging method for functional molecular imaging of tumors targeted to neovascularization, and may be a promising candidate for tumor radioimmunotherapeutic carrier.

语种: 英语
所属项目编号: NSFC 30870729 ; 81071183/H1806 ; 30900374 ; 2006CB705705-1 ; 985-2-056 ; 200800011061
项目资助者: National Natural Science Foundation of China ; National "973" Basic Research Program of China ; National Education Ministry 985 Project of China ; Research Fund for the Doctoral Program of Higher Education of China
WOS记录号: WOS:000300313500008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63196
Appears in Collections:北京大学第一临床医学院_核医学科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Nucl Med, Beijing 100034, Peoples R China
2.Beijing Aerosp Gen Hosp, Radiol Ctr, Beijing 100076, Peoples R China
3.Beijing Normal Univ, Coll Chem, Minist Educ, Key Lab Radiopharmaceut, Beijing 100191, Peoples R China
4.Yangzhou Univ, Clin Med Coll, Dept Nucl Med, Yangzhou 225000, Peoples R China

Recommended Citation:
Lu, Xia,Yan, Ping,Wang, Rong-fu,et al. Use of radioiodinated peptide Arg-Arg-Leu targeted to neovascularization as well as tumor cells in molecular tumor imaging[J]. CHINESE JOURNAL OF CANCER RESEARCH,2012,24(1):52-59.
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