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学科主题: 临床医学
题名:
Recombinant Human Granulocyte Colony-Stimulating Factor Significantly Decreases the Expression of CXCR3 and CCR6 on T Cells and Preferentially Induces T helper Cells to a T helper 17 Phenotype in Peripheral Blood Harvests
作者: Sun, Li-Xia; Ren, Han-Yun; Shi, Yong-Jin; Wang, Li-Hong; Qiu, Zhi-Xiong
关键词: Recombinant human granulocyte colony-stimulating factor ; Chemokine receptors ; IL-17 ; TCRBV
刊名: BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION
发表日期: 2009-07-01
DOI: 10.1016/j.bbmt.2009.03.016
卷: 15, 期:7, 页:835-843
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology ; Immunology ; Transplantation
研究领域[WOS]: Hematology ; Immunology ; Transplantation
关键词[WOS]: VERSUS-HOST-DISEASE ; IN-VIVO ; ALLOGRAFT-REJECTION ; CYTOKINE PRODUCTION ; G-CSF ; GRAFT ; TRANSPLANTATION ; RECEPTOR ; INFLAMMATION ; LYMPHOCYTES
英文摘要:

The aim of this study was to investigate the expression of chemokine receptors on T cells and functional changes of T helper (Th) cells in peripheral blood stem cell (PBSC) harvests after treating healthy donors with recombinant human granulocyte colony-stimulating factor (rhG-CSF). Using multiparameter flow cytometry, we analyzed the expression of CXCR3 and CCR6 on T cells and the production of interferon-gamma (IFN-gamma), interleukin-4 (IL-4), and IL-17 by CD4(+) Th cells in PBSC grafts of healthy donors after in vivo rhG-CSF application. Alterations in the relative expression levels of T cell receptor beta variable (TCRBV) family members were determined using real-time polymerase chain reaction (PCR). rhG-CSF mobilization significantly decreased the expression of CXCR3 and CCR6 on T cells. Treating donors with rhG-CSF resulted in decreased IFN-gamma production and dramatically increased IL-4 and IL-17 secretion by CD4(+) Th cells, leading to T cell polarization from the Th1 to the Th2 phenotype and a preferential increase in IL-17-proclucing CD4(+) Th cells. We did not observe any differences in the relative expression levels of TCRBV family members before and after in vivo rhG-CSF application. Our results suggest that the expression of CXCR3 and CCR6 on donor T cells was dramatically downregulated and an IL-17 phenotype of CD4(+) Th cells was preferentially induced in PBSC grafts after treating healthy donors with rhG-CSF. The observed effects of rhG-CSF on T cells may be independent of the relative expression levels of TCRBV family members.

语种: 英语
WOS记录号: WOS:000267588300009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63214
Appears in Collections:北京大学第一临床医学院_血液内科_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Hematol, Beijing 100034, Peoples R China

Recommended Citation:
Sun, Li-Xia,Ren, Han-Yun,Shi, Yong-Jin,et al. Recombinant Human Granulocyte Colony-Stimulating Factor Significantly Decreases the Expression of CXCR3 and CCR6 on T Cells and Preferentially Induces T helper Cells to a T helper 17 Phenotype in Peripheral Blood Harvests[J]. BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION,2009,15(7):835-843.
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