IR@PKUHSC  > 北京大学药学院
学科主题药学
Studies on modified Oligonucleotides
Zhang, Li He; Yang, Zhen Jun; Mang, Liang Ren
关键词isonucleoside aminonucleoside siRNA antisense oligodeoxynucelotide
刊名PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS
2008-02-01
DOI10.1080/10426500701734539
183期:2-3页:326-334
收录类别ISTP ; SCI
文章类型Proceedings Paper
WOS标题词Science & Technology
类目[WOS]Chemistry, Inorganic & Nuclear ; Chemistry, Organic
研究领域[WOS]Chemistry
关键词[WOS]ANTISENSE OLIGONUCLEOTIDES ; RNA ; OLIGODEOXYNUCLEOTIDES ; INTERFERENCE ; INHIBITION ; POTENT
英文摘要

A series of isonucleotide incorporated antisense oligodeoxynucleotides have been synthesized, in which isonucleotide was introduced at different positions of the sequences. The results showed that though the incorporated isonucleotides at different position of the sequence interfered in the binding ability in different extent, B-form duplexes were maintained and the binding abilities of the X-end modified duplexes were better than the corresponding mismatched duplexes. The DNA IRNA hybrid formed by modified oligodeoxynucleotide and its target RNA could activate the activity of RNase H. The X-end modified antisense oligodeoxynucelotides showed the inhibition on S glycoprotein expression of SARS-CoV at the mRNA levels in insect Sf9 cells. In this presentation, a novel class of amino-isonucleoside was synthesized and incorporated into different positions in the sense or antisense strand of siRNA duplexes. The 3′ and 5′ sense sty-and aminonucleoside modified siRNAs (ssISO-siRNA1 and ssISO-siRNA2) showed the similar duplex thermal and serum stability as natural one and the luciferase activities showed that such modified siRNA is compatible with the intracellular siRNA machinery.

语种英语
WOS记录号WOS:000254838100015
Citation statistics
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63222
Collection北京大学药学院
作者单位Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Li He,Yang, Zhen Jun,Mang, Liang Ren. Studies on modified Oligonucleotides[J]. PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS,2008,183(2-3):326-334.
APA Zhang, Li He,Yang, Zhen Jun,&Mang, Liang Ren.(2008).Studies on modified Oligonucleotides.PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS,183(2-3),326-334.
MLA Zhang, Li He,et al."Studies on modified Oligonucleotides".PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS 183.2-3(2008):326-334.
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