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学科主题: 口腔医学
题名:
Reactive Oxygen Species and Autophagy Associated Apoptosis and Limitation of Clonogenic Survival Induced by Zoledronic Acid in Salivary Adenoid Cystic Carcinoma Cell Line SACC-83
作者: Ge, Xi-Yuan1; Yang, Lin-Qian2; Jiang, Yang2; Yang, Wen-Wen2; Fu, Jia1; Li, Sheng-Lin1,2
刊名: PLOS ONE
发表日期: 2014-06-25
DOI: 10.1371/journal.pone.0101207
卷: 9, 期:6
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: HUMAN FIBROSARCOMA CELLS ; S-PHASE ARREST ; IN-VITRO ; POSTMENOPAUSAL OSTEOPOROSIS ; PANCREATIC-CANCER ; MULTIPLE-MYELOMA ; PROSTATE-CANCER ; BISPHOSPHONATES ; METASTASES ; GROWTH
英文摘要:

Salivary adenoid cystic carcinoma is an epithelial tumor in the head and neck region. Despite its slow growth, patients with salivary adenoid cystic carcinoma exhibit poor long term survival because of a high rate of distant metastasis. Lung and bone are common distant metastasis sites. Zoledronic acid, a third generation bisphosphonate, has been used for tumor-induced osteolysis due to bone metastasis and has direct antitumor activity in several human neoplasms. Here, we observed that zoledronic acid inhibited salivary adenoid cystic carcinoma cell line SACC-83 xenograft tumor growth in nude mice. In vitro, zoledronic acid induced apoptosis and reduced clonogenic survival in SACC-83. Flow cytometry and western blotting indicated that the cell cycle was arrested at G0/G1. Zoledronic acid treatment upregulated reactive oxygen species as well as the autophagy marker protein LC-3B. Reactive oxygen species scavenger N-acetylcysteine and autophagy antagonist 3-methyladenine decreased zoledronic acid-induced apoptosis and increased clonogenic survival. Silencing of the autophagy related gene Beclin-1 also decreased zoledronic acid-induced apoptosis and inhibition of clonogenic formation. In addition, isobolographic analysis revealed synergistic effects on apoptosis when zoledronic acid and paclitaxel/cisplatin were combined. Taken together, our results suggest that zoledronic acid induced apoptosis and reduced clonogenic survival via upregulation of reactive oxygen species and autophagy in the SACC-83 cell line. Thus, zoledronic acid should be considered a promising drug for the treatment of salivary adenoid cystic carcinoma.

语种: 英语
所属项目编号: 81272966 ; 81272967
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000338709500119
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63241
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Cent Lab, Sch & Hosp Stomatol, Beijing 100871, Peoples R China
2.Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Beijing 100871, Peoples R China

Recommended Citation:
Ge, Xi-Yuan,Yang, Lin-Qian,Jiang, Yang,et al. Reactive Oxygen Species and Autophagy Associated Apoptosis and Limitation of Clonogenic Survival Induced by Zoledronic Acid in Salivary Adenoid Cystic Carcinoma Cell Line SACC-83[J]. PLOS ONE,2014,9(6).
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