IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Phosphoproteomic Analysis of Gossypol-Induced Apoptosis in Ovarian Cancer Cell Line, HOC1a
Jin, Lixu1; Chen, Yuling2; Mu, Xinlin3; Lian, Qingquan4,5; Deng, Haiyun4,5; Ge, Renshan4,5
刊名BIOMED RESEARCH INTERNATIONAL
2014
DOI10.1155/2014/123482
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
资助者NSFC ; NSFC
研究领域[WOS]Biotechnology & Applied Microbiology ; Research & Experimental Medicine
关键词[WOS]SMALL-MOLECULE INHIBITORS ; HIPPO-YAP PATHWAY ; PROSTATE-CANCER ; CARCINOMA-CELLS ; RESISTANT OVARIAN ; PROTEIN-KINASES ; GROWTH ; BCL-2 ; ONCOGENE ; PHOSPHORYLATION
英文摘要

Ovarian cancer is a major cause for death of gynecological cancer patients. The efficacy of traditional surgery and chemotherapy is rather compromised and platinum-resistant cancer recurs. Finding new therapeutic targets is urgently needed to increase the survival rate and to improve life quality of patients with ovarian cancer. In the present work, phosphoproteomic analysis was carried out on untreated and gossypol-treated ovarian cancer cell line, HOC1a. We identified approximately 9750 phosphopeptides from 3030 phosphoproteins, which are involved in diverse cellular processes including cytoskeletal organization, RNA and nucleotide binding, and cell cycle regulation. Upon gossypol treatment, changes in phosphorylation of twenty-nine proteins including YAP1 and AKAP12 were characterized. Western blotting and qPCR analysis were used to determine expression levels of proteins in YAP1-related Hippo pathway showing that gossypol induced upregulation of LATS1, which phosphorylates YAP1 at Ser 61. Furthermore, our data showed that gossypol targets the actin cytoskeletal organization through mediating phosphorylation states of actin-binding proteins. Taken together, our data provide valuable information to understand effects of gossypol on protein phosphorylation and apoptosis of ovarian cancer cells.

语种英语
所属项目编号30871434
资助者NSFC ; NSFC
WOS记录号WOS:000340765200001
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63247
专题北京大学第二临床医学院
作者单位1.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
2.Peking Univ, Peoples Hosp, Beijing 100044, Peoples R China
3.Wenzhou Med Univ, Affiliated Hosp 1, Wenzhou 325027, Zhejiang, Peoples R China
4.Wenzhou Med Univ, Affiliated Hosp 2, Wenzhou 325027, Zhejiang, Peoples R China
5.Wenzhou Med Univ, Inst Reprod Biomed, Wenzhou 325027, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714
Jin, Lixu,Chen, Yuling,Mu, Xinlin,et al. Phosphoproteomic Analysis of Gossypol-Induced Apoptosis in Ovarian Cancer Cell Line, HOC1a[J]. BIOMED RESEARCH INTERNATIONAL,2014.
APA Jin, Lixu,Chen, Yuling,Mu, Xinlin,Lian, Qingquan,Deng, Haiyun,&Ge, Renshan.(2014).Phosphoproteomic Analysis of Gossypol-Induced Apoptosis in Ovarian Cancer Cell Line, HOC1a.BIOMED RESEARCH INTERNATIONAL.
MLA Jin, Lixu,et al."Phosphoproteomic Analysis of Gossypol-Induced Apoptosis in Ovarian Cancer Cell Line, HOC1a".BIOMED RESEARCH INTERNATIONAL (2014).
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