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学科主题: 临床医学
题名:
Phosphoproteomic Analysis of Gossypol-Induced Apoptosis in Ovarian Cancer Cell Line, HOC1a
作者: Jin, Lixu1; Chen, Yuling2; Mu, Xinlin3; Lian, Qingquan4,5; Deng, Haiyun4,5; Ge, Renshan4,5
刊名: BIOMED RESEARCH INTERNATIONAL
发表日期: 2014
DOI: 10.1155/2014/123482
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
研究领域[WOS]: Biotechnology & Applied Microbiology ; Research & Experimental Medicine
关键词[WOS]: SMALL-MOLECULE INHIBITORS ; HIPPO-YAP PATHWAY ; PROSTATE-CANCER ; CARCINOMA-CELLS ; RESISTANT OVARIAN ; PROTEIN-KINASES ; GROWTH ; BCL-2 ; ONCOGENE ; PHOSPHORYLATION
英文摘要:

Ovarian cancer is a major cause for death of gynecological cancer patients. The efficacy of traditional surgery and chemotherapy is rather compromised and platinum-resistant cancer recurs. Finding new therapeutic targets is urgently needed to increase the survival rate and to improve life quality of patients with ovarian cancer. In the present work, phosphoproteomic analysis was carried out on untreated and gossypol-treated ovarian cancer cell line, HOC1a. We identified approximately 9750 phosphopeptides from 3030 phosphoproteins, which are involved in diverse cellular processes including cytoskeletal organization, RNA and nucleotide binding, and cell cycle regulation. Upon gossypol treatment, changes in phosphorylation of twenty-nine proteins including YAP1 and AKAP12 were characterized. Western blotting and qPCR analysis were used to determine expression levels of proteins in YAP1-related Hippo pathway showing that gossypol induced upregulation of LATS1, which phosphorylates YAP1 at Ser 61. Furthermore, our data showed that gossypol targets the actin cytoskeletal organization through mediating phosphorylation states of actin-binding proteins. Taken together, our data provide valuable information to understand effects of gossypol on protein phosphorylation and apoptosis of ovarian cancer cells.

语种: 英语
所属项目编号: 30871434
项目资助者: NSFC
WOS记录号: WOS:000340765200001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63247
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
2.Peking Univ, Peoples Hosp, Beijing 100044, Peoples R China
3.Wenzhou Med Univ, Affiliated Hosp 1, Wenzhou 325027, Zhejiang, Peoples R China
4.Wenzhou Med Univ, Affiliated Hosp 2, Wenzhou 325027, Zhejiang, Peoples R China
5.Wenzhou Med Univ, Inst Reprod Biomed, Wenzhou 325027, Zhejiang, Peoples R China

Recommended Citation:
Jin, Lixu,Chen, Yuling,Mu, Xinlin,et al. Phosphoproteomic Analysis of Gossypol-Induced Apoptosis in Ovarian Cancer Cell Line, HOC1a[J]. BIOMED RESEARCH INTERNATIONAL,2014.
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