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RECK overexpression reduces invasive ability in ameloblastoma cells
Liang, Qi-xiang1,2; Liang, Yan-can1,2; Xu, Zhi-ying3; Chen, Wei-liang1; Xie, Hong-liang4; Zhang, Bin1,2
关键词ameloblastoma invasion matrix metalloproteinase reversion-inducing cysteine-rich protein with Kazal motifs
刊名JOURNAL OF ORAL PATHOLOGY & MEDICINE
2014-09-01
DOI10.1111/jop.12179
43期:8页:613-618
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine ; Pathology
研究领域[WOS]Dentistry, Oral Surgery & Medicine ; Pathology
关键词[WOS]CYSTEINE-RICH PROTEIN ; MATRIX-METALLOPROTEINASE REGULATOR ; KAZAL MOTIFS RECK ; COLORECTAL-CANCER ; PROGNOSTIC INDICATOR ; IN-VITRO ; EXPRESSION ; INHIBITOR ; CARCINOMA ; MATRIX-METALLOPROTEINASE-9
英文摘要

BACKGROUND: Ameloblastoma is a frequent odontogenic neoplasm characterized by local invasiveness and high risk of recurrence. Reversion-inducing cysteine-rich protein with Kazal motifs (RECK) is a tumor suppressor that inhibits metastasis and angiogenesis. The aim of this study was to investigate effects of RECK overexpression on invasive potential in ameloblastoma cells.

METHODS: Lentiviral vectors containing human RECK gene were created and subsequently stably transfected into immortalized ameloblastoma cell line hTERT(+)-AM. Functional characteristics of hTERT(+)-AM cells with stable RECK overexpression included proliferation, migration, invasion, and regulation of matrix metalloproteinases (MMP)-2, MMP-9 measured by zymography or commercially available assays.

RESULTS: The stable and higher expression of RECK mRNA and protein (P < 0.01) was detected in RECK-transfected hTERT(+)-AM cells. RECK overexpression caused a decrease in migration and invasion (P < 0.01) for hTERT(+)-AM cells and a decrease in activity of MMP-2, MMP-9 (P < 0.01). Proliferation was not affected by RECK overexpression (P > 0.05).

CONCLUSIONS: Overexpression of RECK gene significantly inhibited cell invasive ability of hTERT(+)-AM cells, suggesting RECK may be a new target for ameloblastoma treatment.

语种英语
WOS记录号WOS:000341664400009
项目编号81072229 ; 06021272
资助机构National Natural Science Foundation of China ; Natural Science Foundation of Guangdong Province
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63255
专题北京大学深圳医院_口腔医学中心
作者单位1.Sun Yat Sen Univ, Dept Oral & Maxillofacial Surg, Sun Yat Sen Mem Hosp, Guangzhou 510120, Guangdong, Peoples R China
2.Sun Yat Sen Univ, Key Lab Malignant Tumor Gene Regulat & Target The, Guangzhou Higher Educ Inst, Guangzhou 510120, Guangdong, Peoples R China
3.Peking Univ, Shenzhen Hosp, Dept Stomatol, Shenzhen, Guangdong, Peoples R China
4.Shenzhen Peoples Hosp, Dept Stomatol, Shenzhen, Guangdong, Peoples R China
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GB/T 7714
Liang, Qi-xiang,Liang, Yan-can,Xu, Zhi-ying,et al. RECK overexpression reduces invasive ability in ameloblastoma cells[J]. JOURNAL OF ORAL PATHOLOGY &amp; MEDICINE,2014,43(8):613-618.
APA Liang, Qi-xiang,Liang, Yan-can,Xu, Zhi-ying,Chen, Wei-liang,Xie, Hong-liang,&Zhang, Bin.(2014).RECK overexpression reduces invasive ability in ameloblastoma cells.JOURNAL OF ORAL PATHOLOGY & MEDICINE,43(8),613-618.
MLA Liang, Qi-xiang,et al."RECK overexpression reduces invasive ability in ameloblastoma cells".JOURNAL OF ORAL PATHOLOGY & MEDICINE 43.8(2014):613-618.
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