IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
KRAS and BRAF gene mutations and DNA mismatch repair status in Chinese colorectal carcinoma patients
Ye, Ju-Xiang1; Liu, Yan1; Qin, Yun1; Zhong, Hao-Hao1; Yi, Wei-Ning2; Shi, Xue-Ying1
关键词Colorectal carcinoma KRAS BRAF DNA mismatch repair MLH1 MSH2 MSH6 PMS2
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2015-02-07
DOI10.3748/wjg.v21.i5.1595
21期:5页:1595-1605
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]K-RAS ; MICROSATELLITE-INSTABILITY ; COLON-CANCER ; ADJUVANT CHEMOTHERAPY ; PIK3CA MUTATIONS ; LYNCH-SYNDROME ; POOR SURVIVAL ; STAGE-II ; POPULATION ; FEATURES
英文摘要

AIM: To investigate gene mutations and DNA mismatch repair (MMR) protein abnormality in Chinese colorectal carcinoma (CRC) patients and their correlations with clinicopathologic features.

METHODS: Clinical and pathological information for 535 patients including 538 tumors was reviewed and recorded. Mutation analyses for exon 2 of KRAS gene and exon 15 of BRAF gene were performed by Sanger sequencing except that in 9 tumors amplification refractory mutation system PCR was used. Expression of MMR proteins including MHL1, MSH2, MSH6 and PMS2 was evaluated by immunohistochemistry. Correlations of KRAS and BRAF mutation status and the expression status of MMR proteins with age, gender, cancer stage, location, and histology were analyzed. Correlations between KRAS or BRAF mutations and MMR protein expression were also explored.

RESULTS: The overall frequencies of KRAS and BRAF mutations were 37.9% and 4.4%, respectively. KRAS mutations were more common in patients >= 50 years old (39.8% vs 22% in patients < 50 years old, P < 0.05). The frequencies of BRAF mutants were higher in tumors from females (6.6% vs males 2.8%, P < 0.05), located in the right colon (9.6% vs 2.1% in the left colon, 1.8% in the rectum, P < 0.01), with mucinous differentiation (9.8% vs 2.8% without mucinous differentiation, P < 0.01), or being poorly differentiated (9.5% vs 3.4% well/moderately differentiated, P < 0.05). MMR deficiency was strongly associated with proximal location (20.5% in the right colon vs 9.2% in the left colon and 5.1% in the rectum, P < 0.001), early cancer stage (15.0% in stages I-II vs 7.7% in stages III-IV, P < 0.05), and mucinous differentiation (20.2% vs 9.2% without mucin, P < 0.01). A higher frequency of MLH1/PMS2 loss was found in females (9.2% vs 4.4% in males, P < 0.05), and MSH2/MSH6 loss tended to be seen in younger (< 50 years old) patients (12.0% vs 4.0% >= 50 years old, P < 0.05). MMR deficient tumors were less likely to have KRAS mutations (18.8% vs 41.7% in MMR proficient tumors, P < 0.05) and tumors with abnormal MLH1/PMS2 tended to harbor BRAF mutations (15.4% vs 4.2% in MMR proficient tumors, P < 0.05).

CONCLUSION: The frequency of sporadic CRCs having BRAF mutation, MLH1 deficiency and MSI in Chinese population may be lower than that in the Western population.

语种英语
WOS记录号WOS:000349665700024
项目编号201233146-14
资助机构Xinjiang Uygur Autonomous Region Natural Science Fund
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63267
专题北京大学第三临床医学院
北京大学基础医学院
北京大学公共卫生学院_公共卫生学院
作者单位1.Peking Univ, Hosp 3, Hlth Sci Ctr, Sch Basic Med Sci,Dept Pathol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Epidemiol & Biostat, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Ye, Ju-Xiang,Liu, Yan,Qin, Yun,et al. KRAS and BRAF gene mutations and DNA mismatch repair status in Chinese colorectal carcinoma patients[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2015,21(5):1595-1605.
APA Ye, Ju-Xiang,Liu, Yan,Qin, Yun,Zhong, Hao-Hao,Yi, Wei-Ning,&Shi, Xue-Ying.(2015).KRAS and BRAF gene mutations and DNA mismatch repair status in Chinese colorectal carcinoma patients.WORLD JOURNAL OF GASTROENTEROLOGY,21(5),1595-1605.
MLA Ye, Ju-Xiang,et al."KRAS and BRAF gene mutations and DNA mismatch repair status in Chinese colorectal carcinoma patients".WORLD JOURNAL OF GASTROENTEROLOGY 21.5(2015):1595-1605.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
KRAS and BRAF gene m(1936KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ye, Ju-Xiang]的文章
[Liu, Yan]的文章
[Qin, Yun]的文章
百度学术
百度学术中相似的文章
[Ye, Ju-Xiang]的文章
[Liu, Yan]的文章
[Qin, Yun]的文章
必应学术
必应学术中相似的文章
[Ye, Ju-Xiang]的文章
[Liu, Yan]的文章
[Qin, Yun]的文章
相关权益政策
暂无数据
收藏/分享
文件名: KRAS and BRAF gene mutations and DNA mismatch repair status in Chinese colorectal carcinoma patients.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。