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学科主题: 基础医学
题名:
Wild type p53 gene causes reorganization of cytoskeleton and, therefore, the impaired deformability and difficult migration of murine erythroleukemia cells
作者: Yao, WJ; Gu, L; Sun, DG; Ka, WB; Wen, ZY; Chien, S
关键词: p53 gene ; cytoskeleton ; murine erythroleukemia cells (MEL) ; deformability ; migration
刊名: CELL MOTILITY AND THE CYTOSKELETON
发表日期: 2003-09-01
DOI: 10.1002/cm.10129\
卷: 56, 期:1, 页:1-12
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: HUMAN CANCER-CELLS ; VISCOELASTIC PROPERTIES ; INFRARED-SPECTROSCOPY ; APOPTOSIS ; DIFFERENTIATION ; POLARIZATION ; RESISTANCE ; MECHANISM ; ARREST ; LINES
英文摘要:

We studied the role of p53 gene in the biophysics and biology in murine erythroleukemia cell line (MEL), with the goal of understanding the influence of this tumor suppressor gene on the deformability and metastasis of tumor cells. Experiments were performed on MEL and p53-transfected MEL (MEL-M with mutant p53 gene and MEL-W with wild-type p53 gene). The cell growth curves indicated that the overexpression of wild-type p53 gene significantly suppressed the growth of MEL, with G(0)-G(1) arrest and apoptosis shown by flow cytometric assays. Confocal laser scanning microscopy revealed that the MEL-W had a more compact organization of the F-Actin cytoskeleton than MEL and MEL-M. Fluorescence polarization measurement indicated a higher membrane fluidity of MEL-W than the other two groups. Fourier transform infrared spectroscopy (FT-IR) showed changes in the composition and/or structure of membrane lipids in MEL-W, with decreases in secondary structures of proteins such as alpha-helix, turns and bends and random coil, in comparison to MEL and MEL-M. The osmotic fragility curves indicated that MEL-W was more fragile and micropipette experiments showed that they had increased elasticity and reduced deformability in comparison to MEL and MEL-M. The adhesion assay with the use of the flow chamber revealed a lower adhesion rate of MEL-W to endothelial cells at high shear stress. The present study on the molecular biology with biophysics of MEL cells contributes to our knowledge on the tumor suppressor gene p53. Cell Motil. Cytoskeleton 56:1-12, 2003. (C) 2003 Wiley-Liss, Inc.

语种: 英语
WOS记录号: WOS:000185108100001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63273
Appears in Collections:基础医学院_北京大学血液流变中心_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Phys Med, Hemorheol Ctr, Beijing 100083, Peoples R China
2.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA

Recommended Citation:
Yao, WJ,Gu, L,Sun, DG,et al. Wild type p53 gene causes reorganization of cytoskeleton and, therefore, the impaired deformability and difficult migration of murine erythroleukemia cells[J]. CELL MOTILITY AND THE CYTOSKELETON,2003,56(1):1-12.
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