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学科主题: 药学
题名:
Bis(acetylacetonato)-oxovanadium(IV), bis(maltolato)-oxovanadium(IV) and sodium metavanadate induce antilipolytic effects by regulating hormone-sensitive lipase and perilipin via activation of Akt
作者: Liu, Jing-Cheng1,2; Yu, You2; Wang, Gang2; Wang, Kui1,2; Yang, Xiao-Gai2
刊名: METALLOMICS
发表日期: 2013
DOI: 10.1039/c3mt00001j
卷: 5, 期:7, 页:813-820
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: HUMAN ADIPOSE-TISSUE ; LIPID-METABOLISM ; ADIPOCYTE DIFFERENTIATION ; FATTY-ACIDS ; KINASE-B ; INSULIN ; LIPOLYSIS ; VANADATE ; CELL ; PHOSPHORYLATION
英文摘要:

The increased plasma free fatty acid levels due to the deregulated lipolysis in adipocytes are considered as one of the major risk factors for developing type II diabetes. Vanadium compounds are well-known for their antidiabetic effects both on glucose and lipid metabolism, but the mechanisms are still not completely understood. The present study suggests a mechanism for how vanadium compounds exert antilipolytic effects. It demonstrates that all the three vanadium compounds, bis(acetylacetonato)-oxovanadium(IV) (VO(acac)(2)), bis(maltolato)-oxovanadium(IV) (VO(ma)(2)) and sodium metavanadate (NaVO3), attenuated basal lipolysis in 3T3L1 adipocytes in a dose- (from 100 to 400 mu M for VO(acac)(2) and VO(ma)(2), 1.0 to 4.0 mM for vanadate) and time-dependent (from 0.5 to 4 h) manner using the glycerol release as a marker of lipolysis. In addition, the three compounds inhibited lipolysis to a different extent. Among them, VO(acac)(2) (from 100 to 400 mu M) exerted the most potent effect and reduced the lipolysis to similar to 60-20% of control after 4 h treatment. The antilipolytic effects of vanadium compounds were further evidenced by a decrease of the levels of phosphorylated HSL at Ser660 and phosphorylated perilipin, which were counteracted by inhibitors of PI3K or Akt but not by an MEK inhibitor. This indicates that though both Akt and ERK pathways are activated by the vanadium compounds, only Akt activation contributes to the antilipolytic effect of the vanadium compounds, without the involvement of ERK activation. We previously demonstrated that VO(acac)(2) can block cell cycle progression at the G1/S phase via a highly activated ERK signal in human hepatoma HepG2 cells. Together with this study, we show that similar activated pathways may lead to differential biological consequences for cancer cells and adipocytes, indicating that vanadium compounds may be used in the prevention and treatment of both diabetes and cancer.

语种: 英语
所属项目编号: 20871008 ; 21171011
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000320951600006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63283
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China

Recommended Citation:
Liu, Jing-Cheng,Yu, You,Wang, Gang,et al. Bis(acetylacetonato)-oxovanadium(IV), bis(maltolato)-oxovanadium(IV) and sodium metavanadate induce antilipolytic effects by regulating hormone-sensitive lipase and perilipin via activation of Akt[J]. METALLOMICS,2013,5(7):813-820.
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