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学科主题: 临床医学
题名:
The effects of quercetin and kaempferol on multidrug resistance and the expression of related genes in human erythroleukemic K562/A cells
作者: Han Yanqiu1; Cao Linjuan1; Wu Jin1; Hao Hongjun2; Shi Yongjin3; Xu Guobin4; Ren Hanyun3
关键词: Flavonoids ; leukemia ; multidrug resistance ; polymerase chain reaction (PCR) array
刊名: AFRICAN JOURNAL OF BIOTECHNOLOGY
发表日期: 2011-10-12
卷: 10, 期:62, 页:13399-13406
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
关键词[WOS]: HUMAN LEUKEMIA-CELLS ; MEMBRANE TRANSPORTERS ; P-GLYCOPROTEIN ; FLAVONOIDS ; APOPTOSIS ; CHEMOSENSITIVITY ; CHEMORESISTANCE ; CHANNELS ; ARREST
英文摘要:

Leukemia chemotherapy is believed to be impeded by multidrug resistance (MDR). Some compounds of flavonoid molecules were previously shown to inhibit drug transporter Pgp or induce apoptosis to sensitize MDR tumors. In this study, we attempted to investigate the possibility and mechanism of quercetin and kaempferol, flavonoid molecules, in reversing MDR. K562/A cells were cultured in vitro with the flavonoids as single and in combination respectively. Cell growth inhibition and adriamycin (ADR) sensitivity were detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Cell apoptosis was examined by Annexin V/PI staining method. Moreover, the expression of related genes for drug transporters and apoptosis was also tested after incubation with resveratrol for the first time. Results show a dose dependent manner and synergistic effect of the two compounds on K562/A. Furthermore, some of the genes in drug transporter families such as ATP-binding cassette (ABC) and solute carrier (SLC) and apoptosis related genes such as Bcl-2. tumor necrosis factor (TNF) and tumor necrosis factor receptor (TNFR) families were regulated. The experiment indicates that quercetin and kaempferol may be used as reveratrol in leukemia chemotherapy, but their interaction and difference should be noticed.

语种: 英语
项目资助者: Innermongolian Natural Science ; Affiliated Hospital of Innermongolian Medical College
WOS记录号: WOS:000298546900006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63294
Appears in Collections:北京大学第一临床医学院_神经内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Neurol, Beijing, Peoples R China
2.Peking Univ, Hosp 1, Dept Hematol, Beijing, Peoples R China
3.Peking Univ, Hosp 1, Dept Ecsomat, Beijing, Peoples R China
4.Innermongolian Med Coll, Affiliated Hosp, Dept Hematol, Hohhot, Peoples R China

Recommended Citation:
Han Yanqiu,Cao Linjuan,Wu Jin,et al. The effects of quercetin and kaempferol on multidrug resistance and the expression of related genes in human erythroleukemic K562/A cells[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(62):13399-13406.
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