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学科主题临床医学
The effects of quercetin and kaempferol on multidrug resistance and the expression of related genes in human erythroleukemic K562/A cells
Han Yanqiu1; Cao Linjuan1; Wu Jin1; Hao Hongjun2; Shi Yongjin3; Xu Guobin4; Ren Hanyun3
关键词Flavonoids leukemia multidrug resistance polymerase chain reaction (PCR) array
刊名AFRICAN JOURNAL OF BIOTECHNOLOGY
2011-10-12
10期:62页:13399-13406
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]HUMAN LEUKEMIA-CELLS ; MEMBRANE TRANSPORTERS ; P-GLYCOPROTEIN ; FLAVONOIDS ; APOPTOSIS ; CHEMOSENSITIVITY ; CHEMORESISTANCE ; CHANNELS ; ARREST
英文摘要

Leukemia chemotherapy is believed to be impeded by multidrug resistance (MDR). Some compounds of flavonoid molecules were previously shown to inhibit drug transporter Pgp or induce apoptosis to sensitize MDR tumors. In this study, we attempted to investigate the possibility and mechanism of quercetin and kaempferol, flavonoid molecules, in reversing MDR. K562/A cells were cultured in vitro with the flavonoids as single and in combination respectively. Cell growth inhibition and adriamycin (ADR) sensitivity were detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Cell apoptosis was examined by Annexin V/PI staining method. Moreover, the expression of related genes for drug transporters and apoptosis was also tested after incubation with resveratrol for the first time. Results show a dose dependent manner and synergistic effect of the two compounds on K562/A. Furthermore, some of the genes in drug transporter families such as ATP-binding cassette (ABC) and solute carrier (SLC) and apoptosis related genes such as Bcl-2. tumor necrosis factor (TNF) and tumor necrosis factor receptor (TNFR) families were regulated. The experiment indicates that quercetin and kaempferol may be used as reveratrol in leukemia chemotherapy, but their interaction and difference should be noticed.

语种英语
WOS记录号WOS:000298546900006
资助机构Innermongolian Natural Science ; Affiliated Hospital of Innermongolian Medical College
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63294
专题北京大学第一临床医学院_神经内科
北京大学第一临床医学院_血液内科
作者单位1.Peking Univ, Hosp 1, Dept Neurol, Beijing, Peoples R China
2.Peking Univ, Hosp 1, Dept Hematol, Beijing, Peoples R China
3.Peking Univ, Hosp 1, Dept Ecsomat, Beijing, Peoples R China
4.Innermongolian Med Coll, Affiliated Hosp, Dept Hematol, Hohhot, Peoples R China
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GB/T 7714
Han Yanqiu,Cao Linjuan,Wu Jin,et al. The effects of quercetin and kaempferol on multidrug resistance and the expression of related genes in human erythroleukemic K562/A cells[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(62):13399-13406.
APA Han Yanqiu.,Cao Linjuan.,Wu Jin.,Hao Hongjun.,Shi Yongjin.,...&Ren Hanyun.(2011).The effects of quercetin and kaempferol on multidrug resistance and the expression of related genes in human erythroleukemic K562/A cells.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(62),13399-13406.
MLA Han Yanqiu,et al."The effects of quercetin and kaempferol on multidrug resistance and the expression of related genes in human erythroleukemic K562/A cells".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.62(2011):13399-13406.
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