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学科主题: 临床医学
题名:
The Abnormally High Level of Uric D-Ribose for Type-2 Diabetics
作者: Su Tao1,2; Xin Liang1; He Ying-Ge1; Wei Yan1; Song Yi-Xiang3; Li Wei-Wei3; Wang Xue-Mei3; He Rong-Qiao1
关键词: D-ribose ; D-glucose ; glycation ; type 2 diabetes mellitus ; complications ; metabolism disorders
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2013-09-01
DOI: 10.3724/SP.J.1206.2013.00341
卷: 40, 期:9, 页:816-825
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: GLYCATION END-PRODUCTS ; INSULIN ; DISEASE
英文摘要:

In 1815, the French chemist Michel Eugene Chevreul (1786-1889) discovered that the sweetness in the urine of diabetics comes from grape sugar or D-glucose. Diabetes mellitus (DM) is considered as a group of metabolic diseases characterized by hyperglycemia (high concentration of blood D-glucose) resulting from defects in insulin secretion, insulin action, or both. On the other hand, D-ribose as an energetic enhancer was found to decrease the concentration of blood D-glucose, and thus "Oral administration of D-ribose in diabetes mellitus" was ever described by Steinberg and colleagues (1970). As described previously in this laboratory, D-ribose rapidly glycates proteins, such as BSA, neuronal Tau and alpha-synuclein, producing advanced glycation end products (AGEs) with severe cytotoxicity, leading to dysfunction and cell death, in vitro and in vivo. Intraperitoneal injection of D-ribose into mice significantly increases their glycated serum protein and blood AGEs though the concentration of D-glucose became slightly decreased, suggesting that D-ribose is much easier to produce AGEs than D-glucose in vivo. Here, using 4-(3-Methyl-5-oxo-2-pyrazolin-1-yl) benzoic acid (MOPBA) coupled with HPLC, we determined the concentration of uric D-ribose of type 2 diabetic patients (n=30) and the age-matched healthy controls (n=30). The results show that the yield of the derivative of MOPBA-ribose is linearly correlated with the concentration of D-ribose (r(2)=0.999) with a recovery of 99%. The isolated fractions of D-ribose and D-glucose from urine of type 2 diabetic patients through HPLC were analyzed by mass spectrometry, and the results showed that the fractions contained 569.19 u compound (C27H29N4O10, D-ribose), and 599.20 u compound (C28H31N4O11, D-glucose) respectively. The concentration of uric D-ribose of Type 2 diabetics (male (134.28 +/- 35.09) mu mol/L, female (97.33 +/- 23.68) mu mol/L) was significantly (P < 0.001) higher than that of the age-matched healthy control (male (35.99 +/- 5.64) mu mol/L, female (33.72 +/- 6.27) mu mol/L). Under the experimental conditions, the uric D-glucose level of the patients was also markedly (P < 0.001) higher than the control. Further analyses showed a marked increase in the level of uric D-ribose from either male (P < 0.001) or female (P < 0.001), but a significant difference of the uric levels between male and female could not be observed (P> 0.05). The high levels of uric D-ribose and D-glucose of the patients suggest that type 2 diabetic patients are not only suffered from D-glucose metabolism disorders, but also from D-ribose metabolism disorders.

语种: 中文
所属项目编号: 2012CB911000 ; 2010CB912303 ; 31270868 ; 31200601 ; 81100610 ; 5112024
项目资助者: National Basic Research Program of China ; National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation
WOS记录号: WOS:000327809400005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63304
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Beijing 100034, Peoples R China
2.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Su Tao,Xin Liang,He Ying-Ge,et al. The Abnormally High Level of Uric D-Ribose for Type-2 Diabetics[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2013,40(9):816-825.
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