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学科主题: 临床医学
题名:
Establishment of a novel diagnostic model for Sjogren′s syndrome by proteomic fingerprinting
作者: Li, Yuhui; Sun, Xiaolin; Zhang, Xuewu; Yang, Yuqin; Jia, Rulin; Liu, Xu; Li, Ru; Liu, Yanying; Li, Zhanguo
关键词: Diagnostic model Sjogren&prime ; s syndrome ; MALDI-TOF-MS ; Proteomic fingerprinting ; Sjogren&prime ; s syndrome
刊名: CLINICAL RHEUMATOLOGY
发表日期: 2014-12-01
DOI: 10.1007/s10067-014-2762-4
卷: 33, 期:12, 页:1745-1750
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Rheumatology
研究领域[WOS]: Rheumatology
关键词[WOS]: CLASSIFICATION CRITERIA ; AMERICAN-COLLEGE ; RHEUMATOID-ARTHRITIS ; SALIVARY BIOMARKERS ; MASS-SPECTROMETRY ; CANCER ; SERUM ; MORTALITY ; PROTEINS ; PATTERNS
英文摘要:

Primary Sjogren′s syndrome (pSS) is a systemic autoimmune disease that lacks sensitive and specific diagnostic methods. The aim of this study was to identify potential biomarkers specific for pSS and to establish a diagnostic model. Serum samples from patients with pSS, disease controls (DC, patients with systemic lupus erythematosus (SLE), rheumatoid arthritis (RA)), and healthy controls (HC)) were randomly divided into a training set (35 pSS, 50 DC, and 26 HC) and a testing set (25 pSS, 50 DC, and 25 HC). Weak cationic exchange (WCX) magnetic beads were used to differentially capture serum proteins prior to proteomic analysis. Proteomic mass spectra were generated by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). One hundred differential M/Z peaks associated with pSS were identified, and the m/z peaks at 8,133.85, 11,972.8, 2,220.81, and 4,837.66 were used to establish a diagnostic model for pSS. This diagnostic model was able to distinguish pSS from non-pSS controls with a sensitivity of 77.1 % and a specificity of 85.5 %, and its efficacy was confirmed in our blinded testing set with good sensitivity and specificity of 95.5 and 88 %, respectively. The results indicated that the proteomic fingerprinting model was effective in the diagnosis of pSS.

语种: 英语
所属项目编号: 2014BAI07B01 ; Z141107002514064 ; 81273292 ; 81202343 ; RDB2014-03 ; RDB2014-04
项目资助者: National Sci-Tech Support Program ; Beijing Municipal Science &amp ; Technology Commission ; National Natrual Science Foundation of China ; Research and Development Fund of Peking University People&prime ; s Hospital
WOS记录号: WOS:000345091900007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63309
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: Peking Univ, Dept Rheumatol & Immunol, Clin Immunol Ctr, Peoples Hosp, Beijing 100044, Peoples R China

Recommended Citation:
Li, Yuhui,Sun, Xiaolin,Zhang, Xuewu,et al. Establishment of a novel diagnostic model for Sjogren′s syndrome by proteomic fingerprinting[J]. CLINICAL RHEUMATOLOGY,2014,33(12):1745-1750.
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