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Establishment of a novel diagnostic model for Sjogren′s syndrome by proteomic fingerprinting
Li, Yuhui; Sun, Xiaolin; Zhang, Xuewu; Yang, Yuqin; Jia, Rulin; Liu, Xu; Li, Ru; Liu, Yanying; Li, Zhanguo
关键词Diagnostic model Sjogren&prime s syndrome MALDI-TOF-MS Proteomic fingerprinting Sjogren&prime s syndrome
刊名CLINICAL RHEUMATOLOGY
2014-12-01
DOI10.1007/s10067-014-2762-4
33期:12页:1745-1750
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Rheumatology
研究领域[WOS]Rheumatology
关键词[WOS]CLASSIFICATION CRITERIA ; AMERICAN-COLLEGE ; RHEUMATOID-ARTHRITIS ; SALIVARY BIOMARKERS ; MASS-SPECTROMETRY ; CANCER ; SERUM ; MORTALITY ; PROTEINS ; PATTERNS
英文摘要

Primary Sjogren′s syndrome (pSS) is a systemic autoimmune disease that lacks sensitive and specific diagnostic methods. The aim of this study was to identify potential biomarkers specific for pSS and to establish a diagnostic model. Serum samples from patients with pSS, disease controls (DC, patients with systemic lupus erythematosus (SLE), rheumatoid arthritis (RA)), and healthy controls (HC)) were randomly divided into a training set (35 pSS, 50 DC, and 26 HC) and a testing set (25 pSS, 50 DC, and 25 HC). Weak cationic exchange (WCX) magnetic beads were used to differentially capture serum proteins prior to proteomic analysis. Proteomic mass spectra were generated by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). One hundred differential M/Z peaks associated with pSS were identified, and the m/z peaks at 8,133.85, 11,972.8, 2,220.81, and 4,837.66 were used to establish a diagnostic model for pSS. This diagnostic model was able to distinguish pSS from non-pSS controls with a sensitivity of 77.1 % and a specificity of 85.5 %, and its efficacy was confirmed in our blinded testing set with good sensitivity and specificity of 95.5 and 88 %, respectively. The results indicated that the proteomic fingerprinting model was effective in the diagnosis of pSS.

语种英语
WOS记录号WOS:000345091900007
项目编号2014BAI07B01 ; Z141107002514064 ; 81273292 ; 81202343 ; RDB2014-03 ; RDB2014-04
资助机构National Sci-Tech Support Program ; Beijing Municipal Science &amp ; Technology Commission ; National Natrual Science Foundation of China ; Research and Development Fund of Peking University People&prime ; s Hospital
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63309
专题北京大学第二临床医学院
北京大学第二临床医学院_风湿免疫科
作者单位Peking Univ, Dept Rheumatol & Immunol, Clin Immunol Ctr, Peoples Hosp, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Li, Yuhui,Sun, Xiaolin,Zhang, Xuewu,et al. Establishment of a novel diagnostic model for Sjogren′s syndrome by proteomic fingerprinting[J]. CLINICAL RHEUMATOLOGY,2014,33(12):1745-1750.
APA Li, Yuhui.,Sun, Xiaolin.,Zhang, Xuewu.,Yang, Yuqin.,Jia, Rulin.,...&Li, Zhanguo.(2014).Establishment of a novel diagnostic model for Sjogren′s syndrome by proteomic fingerprinting.CLINICAL RHEUMATOLOGY,33(12),1745-1750.
MLA Li, Yuhui,et al."Establishment of a novel diagnostic model for Sjogren′s syndrome by proteomic fingerprinting".CLINICAL RHEUMATOLOGY 33.12(2014):1745-1750.
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