|Humoral immune responses induced by anti-idiotypic antibody fusion protein of 6B11scFv/hGM-CSF in BALB/c mice|
|Chang, XH; Ye, X; Cui, H; Feng, J; Li, Y; Zhu, HI; Yang, WI; Fu, TY; Cheng, HY; Guo, HF|
|关键词||anti-idiotypic antibody ovarian carcinoma recombinant fusion protein humoral immune responses BALB/c mice|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||OVARIAN-CARCINOMA ; GM-CSF ; IMMUNOLOGICAL RESPONSES ; MELANOMA PATIENTS ; TRANSGENIC MICE ; VACCINE ACA125 ; ANTIGEN ; CANCER ; MIMICKING ; DISEASE|
Background We have previously developed and characterized a monoclonal anti-idiotype antibody, designated 6B11, which mimics an ovarian carcinoma associated antigen OC166-9 and whose corresponding monoclonal antibody is COC166-9 (Ab1). In this study, we evaluate the humoral immune responses induced by the fusion protein 6B11 single-chain variable fragment (scFv)/human granulocyte macrophage colony-stimulating factor (hGM-CSF) and 6B11scFv in BALB/c mice.
Methods The fusion protein 6B11scFv/hGM-CSF was constructed by fusing a recombinant single-chain variable fragment of 6B11scFv to GM-CSF. BALB/c mice were administrated by 6B11scFv/hGM-CSF and 6B11scFv, respectively.
Results The fusion protein 6B11scFv/hGM-CSF retained binding to the anti-mouse F(ab)(2)′ and was also biologically active as measured by proliferation of human GM-CSF dependent cell TF1 in vitro. After immunization with the 6B11scFv/hGM-CSF and 6B11ScFv, BALB/c mice showed significantly enhanced Ab3 antibody responses to 6B11scFv/hGM-CSF compared with the 6B11scFv alone. The level of Ab3 was the highest after the first week and maintained for five weeks after the last immunization. Another booster was given when the Ab3 titer descended, and it would reach to the high level in a week.
Conclusion The fusion protein 6B11scFv/hGM-CSF can induce humoral immunity against ovarian carcinoma in vivo. We also provide the theoretical foundation for the application of the fusion protein 6B11scFv/hGM-CSF for active immunotherapy of ovarian cancer.
|作者单位||Peking Univ Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China|
|Chang, XH,Ye, X,Cui, H,et al. Humoral immune responses induced by anti-idiotypic antibody fusion protein of 6B11scFv/hGM-CSF in BALB/c mice[J]. CHINESE MEDICAL JOURNAL,2006,119(2):131-139.|
|APA||Chang, XH.,Ye, X.,Cui, H.,Feng, J.,Li, Y.,...&Guo, HF.(2006).Humoral immune responses induced by anti-idiotypic antibody fusion protein of 6B11scFv/hGM-CSF in BALB/c mice.CHINESE MEDICAL JOURNAL,119(2),131-139.|
|MLA||Chang, XH,et al."Humoral immune responses induced by anti-idiotypic antibody fusion protein of 6B11scFv/hGM-CSF in BALB/c mice".CHINESE MEDICAL JOURNAL 119.2(2006):131-139.|