北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 妇科肿瘤中心  > 期刊论文
学科主题: 临床医学
题名:
Humoral immune responses induced by anti-idiotypic antibody fusion protein of 6B11scFv/hGM-CSF in BALB/c mice
作者: Chang, XH; Ye, X; Cui, H; Feng, J; Li, Y; Zhu, HI; Yang, WI; Fu, TY; Cheng, HY; Guo, HF
关键词: anti-idiotypic antibody ; ovarian carcinoma ; recombinant fusion protein ; humoral immune responses ; BALB/c mice
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2006-01-20
卷: 119, 期:2, 页:131-139
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: OVARIAN-CARCINOMA ; GM-CSF ; IMMUNOLOGICAL RESPONSES ; MELANOMA PATIENTS ; TRANSGENIC MICE ; VACCINE ACA125 ; ANTIGEN ; CANCER ; MIMICKING ; DISEASE
英文摘要:

Background We have previously developed and characterized a monoclonal anti-idiotype antibody, designated 6B11, which mimics an ovarian carcinoma associated antigen OC166-9 and whose corresponding monoclonal antibody is COC166-9 (Ab1). In this study, we evaluate the humoral immune responses induced by the fusion protein 6B11 single-chain variable fragment (scFv)/human granulocyte macrophage colony-stimulating factor (hGM-CSF) and 6B11scFv in BALB/c mice.

Methods The fusion protein 6B11scFv/hGM-CSF was constructed by fusing a recombinant single-chain variable fragment of 6B11scFv to GM-CSF. BALB/c mice were administrated by 6B11scFv/hGM-CSF and 6B11scFv, respectively.

Results The fusion protein 6B11scFv/hGM-CSF retained binding to the anti-mouse F(ab)(2)′ and was also biologically active as measured by proliferation of human GM-CSF dependent cell TF1 in vitro. After immunization with the 6B11scFv/hGM-CSF and 6B11ScFv, BALB/c mice showed significantly enhanced Ab3 antibody responses to 6B11scFv/hGM-CSF compared with the 6B11scFv alone. The level of Ab3 was the highest after the first week and maintained for five weeks after the last immunization. Another booster was given when the Ab3 titer descended, and it would reach to the high level in a week.

Conclusion The fusion protein 6B11scFv/hGM-CSF can induce humoral immunity against ovarian carcinoma in vivo. We also provide the theoretical foundation for the application of the fusion protein 6B11scFv/hGM-CSF for active immunotherapy of ovarian cancer.

语种: 英语
WOS记录号: WOS:000235055200007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63317
Appears in Collections:北京大学第二临床医学院_妇科肿瘤中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China

Recommended Citation:
Chang, XH,Ye, X,Cui, H,et al. Humoral immune responses induced by anti-idiotypic antibody fusion protein of 6B11scFv/hGM-CSF in BALB/c mice[J]. CHINESE MEDICAL JOURNAL,2006,119(2):131-139.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Chang, XH]'s Articles
[Ye, X]'s Articles
[Cui, H]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Chang, XH]‘s Articles
[Ye, X]‘s Articles
[Cui, H]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace