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学科主题基础医学
TMEM166, a novel transmembrane protein, regulates cell autophagy and apoptosis
Wang, Lan; Yu, Chuanfei; Lu, Yang; He, Pengfei; Guo, Jinhai; Zhang, Chenying; Song, Quansheng; Ma, Dalong; Shi, Taiping; Chen, Yingyu
关键词autophagy apoptosis programmed cell death TMEM166 FLJ13391
刊名APOPTOSIS
2007-08-01
DOI10.1007/s10495-007-0073-9
12期:8页:1489-1502
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]TUMOR-SUPPRESSOR PTEN ; DAP-KINASE ; DEATH ; MACROAUTOPHAGY ; PATHWAY ; INHIBITION ; INDUCTION ; MODULATOR ; CASPASES ; SURVIVAL
英文摘要

Programmed cell death can be divided into apoptosis and autophagic cell death. We describe the biological activities of TMEM166 (transmembrane protein 166, also known as FLJ13391), which is a novel lysosome and endoplasmic reticulum-associated membrane protein containing a putative TM domain. Overexpression of TMEM166 markedly inhibited colony formation in HeLa cells. Simultaneously, typical morphological characteristics consistent with autophagy were observed by transmission electron microscopy, including extensive autophagic vacuolization and enclosure of cell organelles by double-membrane structures. Further experiments confirmed that the overexpression of TMEM166 increased the punctate distribution of MDC staining and GFP-LC3 in HeLa cells, as well as the LC3-II/LC3-I proportion. On the other hand, TMEM166-transfected HeLa and 293T cells succumbed to cell death with hallmarks of apoptosis including phosphatidylserine externalization, loss of mitochondrial transmembrane potential, caspase activation and chromatin condensation. Kinetic analysis revealed that the appearance of autophagy-related biochemical parameters preceded the nuclear changes typical of apoptosis in TMEM166-transfected HeLa cells. Suppression of TMEM166 expression by small interference RNA inhibited starvation-induced autophagy in HeLa cells. These findings show for the first time that TMEM166 is a novel regulator involved in both autophagy and apoptosis. Programmed cell death can be divided into apoptosis and autophagic cell death. We describe the biological activities of TMEM166 (transmembrane protein 166, also known as FLJ13391), which is a novel lysosome and endoplasmic reticulum-associated membrane protein containing a putative TM domain. Overexpression of TMEM166 markedly inhibited colony formation in HeLa cells. Simultaneously, typical morphological characteristics consistent with autophagy were observed by transmission electron microscopy, including extensive autophagic vacuolization and enclosure of cell organelles by double-membrane structures. Further experiments confirmed that the overexpression of TMEM166 increased the punctate distribution of MDC staining and GFP-LC3 in HeLa cells, as well as the LC3-II/LC3-I proportion. On the other hand, TMEM166-transfected HeLa and 293T cells succumbed to cell death with hallmarks of apoptosis including phosphatidylserine externalization, loss of mitochondrial transmembrane potential, caspase activation and chromatin condensation. Kinetic analysis revealed that the appearance of autophagy-related biochemical parameters preceded the nuclear changes typical of apoptosis in TMEM166-transfected HeLa cells. Suppression of TMEM166 expression by small interference RNA inhibited starvation-induced autophagy in HeLa cells. These findings show for the first time that TMEM166 is a novel regulator involved in both autophagy and apoptosis.

语种英语
WOS记录号WOS:000247467100012
引用统计
被引频次:46[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63321
专题基础医学院_免疫学系
作者单位1.Peking Univ, Sch Basic Med Sci, Hlth Sci Ctr, Lab Med Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China
3.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
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Wang, Lan,Yu, Chuanfei,Lu, Yang,et al. TMEM166, a novel transmembrane protein, regulates cell autophagy and apoptosis[J]. APOPTOSIS,2007,12(8):1489-1502.
APA Wang, Lan.,Yu, Chuanfei.,Lu, Yang.,He, Pengfei.,Guo, Jinhai.,...&Chen, Yingyu.(2007).TMEM166, a novel transmembrane protein, regulates cell autophagy and apoptosis.APOPTOSIS,12(8),1489-1502.
MLA Wang, Lan,et al."TMEM166, a novel transmembrane protein, regulates cell autophagy and apoptosis".APOPTOSIS 12.8(2007):1489-1502.
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