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学科主题: 临床医学
题名:
Mesenchymal stem cells in stem cell transplant recipients are damaged and remain of host origin
作者: Wang, J; Liu, KY; Lu, DP
关键词: mesenchymal stem cells ; expansion capacity ; origin ; stem cell transplant
刊名: INTERNATIONAL JOURNAL OF HEMATOLOGY
发表日期: 2005-08-01
DOI: 10.1532/IJH97.A10505
卷: 82, 期:2, 页:152-158
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology
研究领域[WOS]: Hematology
关键词[WOS]: BONE-MARROW TRANSPLANTATION ; ACUTE MYELOGENOUS LEUKEMIA ; STROMAL CELLS ; PERIPHERAL-BLOOD ; AMELOGENIN ; ENGRAFTMENT ; BUSULFAN ; CULTURES ; FAILURE ; INVITRO
英文摘要:

The aim of this study was to investigate the expansion capacity and origin of bone marrow-derived mesenchymal stem cells (MSCs) in 34 patients who received a sex-mismatched stem cell transplant (SCT). Polymerase chain reaction (PCR) analysis of the amelogenin gene (AMEL) was used to detect donor-derived MSCs. Cultured MSCs were hybridized with fluorescence in situ hybridization (FISH) probes for chromosomes X and Y to distinguish cells of donor origin from those of host origin. The MSCs of 31 of the 34 patients showed confluent stroma, and the MSCs from 24 of these 31 patients were successfully passaged more than 5 times and were able to be used for PCR and FISH analyses. The colony-forming unit-fibroblast, confluence time, and passage numbers of the MSCs and the colony-forming capacity of the hematopoietic progenitor cells of the patients were significantly different from those of 30 healthy control subjects. Flow cytometry results showed that the proportion of CD14(+)CD45(+) cells, which are regarded as monocytes/macrophages, in cultured MSCs (fifth passage) was less than 0.04%. PCR and FISH analyses revealed that the MSC-derived cells in all 24 patients were from the host. In conclusion, the expansion capacity of MSCs in patients who receive an SCT is damaged, and the MSCs originate from the host.

语种: 英语
WOS记录号: WOS:000231530000013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63343
Appears in Collections:北京大学第二临床医学院_血液科_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Inst Hematol, Beijing 100044, Peoples R China

Recommended Citation:
Wang, J,Liu, KY,Lu, DP. Mesenchymal stem cells in stem cell transplant recipients are damaged and remain of host origin[J]. INTERNATIONAL JOURNAL OF HEMATOLOGY,2005,82(2):152-158.
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