IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Induction of dedifferentiated male mouse adipose stromal vascular fraction cells to primordial germ cell-like cells
Cui, Guanghui1; Qi, Zhengyu; Zhang, Yanmin; Long, Xia; Qin, Jie; Guo, Xin
关键词dedifferentiation embryonic stem cell epiblast cell imprint erasure primordial germ cell stromal vascular fraction cell
刊名CELL BIOLOGY INTERNATIONAL
2014-09-01
DOI10.1002/cbin.10305
38期:9页:1069-1075
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
资助者China National Natural Science Foundation ; China National Natural Science Foundation
研究领域[WOS]Cell Biology
关键词[WOS]EMBRYONIC STEM-CELLS ; BONE-MARROW ; TISSUE ; DERIVATION ; FIBROBLASTS ; TESTIS ; VITRO ; FAT
英文摘要

The adipose stromal vascular fraction (SVF) contains abundant mesenchymal stem cell populations that have a limited ability to self-renew and differentiate. Male mouse adipose SVF cells were dedifferentiated by reprogramming factors (c-Myc, Oct4, Sox2, and Klf4) to form embryonic stem cell-like cells (ESCLCs), which upgraded their limited differentiation potential. The ESCLCs were induced to differentiate toward epiblast-like cells (EpiLCs) and primordial germ cell-like cells (PGCLCs) by culturing in media supplied with activin A and BMP-4, respectively. The derived ESCLCs possess embryonic stem cell features and can automatically form embryonic bodies. After culture in EpiLC induction medium for 2-3 days, ESCLCs formed flattened epithelial structures that were different from their original water drop-like colonies, and the expression of pluripotency-related genes decreased. When the cells that had been cultured in EpiLC induction medium for 2 days were isolated and cultured in PGCLC induction medium for 4-6 days, they formed typical water drop-like colonies again. Moreover, expression of the pluripotency-related genes and the primordial germ cell (PGC) specification-related genes increased. During progression from ESCLCs toward EpiLCs and PGCLCs, the levels of histone methylases H3K9me2 and H3K27me3 kept changing, which resembled those seen in PGC specification. The derived PGCLCs expressed SSEA-1, Blimp-1, and Stella. Furthermore, methylation of Igf2r and Snrpn was retained, but H19 and Kcnq1ot1 methylation levels were slightly reduced compared to non-PGCLCs, suggesting that the derived PGCLCs may have initiated the process of imprint erasure.

语种英语
所属项目编号81070530 ; 81270748
资助者China National Natural Science Foundation ; China National Natural Science Foundation
WOS记录号WOS:000340256500009
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63350
专题北京大学深圳医院
作者单位1.Peking Univ, Med Ctr, Shenzhen Hosp, Guangdong Key Lab Male Reprod Med & Genet, Shenzhen 518036, Guangdong, Peoples R China
2.Hong Kong Sci & Technol Univ, Shenzhen 518036, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Cui, Guanghui,Qi, Zhengyu,Zhang, Yanmin,et al. Induction of dedifferentiated male mouse adipose stromal vascular fraction cells to primordial germ cell-like cells[J]. CELL BIOLOGY INTERNATIONAL,2014,38(9):1069-1075.
APA Cui, Guanghui,Qi, Zhengyu,Zhang, Yanmin,Long, Xia,Qin, Jie,&Guo, Xin.(2014).Induction of dedifferentiated male mouse adipose stromal vascular fraction cells to primordial germ cell-like cells.CELL BIOLOGY INTERNATIONAL,38(9),1069-1075.
MLA Cui, Guanghui,et al."Induction of dedifferentiated male mouse adipose stromal vascular fraction cells to primordial germ cell-like cells".CELL BIOLOGY INTERNATIONAL 38.9(2014):1069-1075.
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