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Simultaneous LC determination of major constituents in red and white peony root
Wang, Q; Liu, RX; Guo, HZ; Ye, M; Huo, CH; Bi, KS; Guo, D
关键词column liquid chromatography red and white peony roots Paeonia lactiflora Pall Paeonia veitchii Lynch
刊名CHROMATOGRAPHIA
2005-12-01
DOI10.1365/s10337-005-0660-2
62期:11-12页:581-588
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PAEONIAE RADIX ; QUANTITATIVE-ANALYSIS ; MOUTAN CORTEX ; CRUDE DRUGS ; PAEONIFLORIN ; CHROMATOGRAPHY ; INHIBITION ; ALBIFLORIN
英文摘要

A reversed phase high performance liquid chromatography method was established for the simultaneous determination of eight major constituents, namely gallic acid, paeoniflorin sulfonate, catechin, albiflorin, paeoniflorin, benzoic acid, pentagalloylglucose and benzoylpaeoniflorin in red and white peony roots, the two commonly used traditional Chinese medicinal herbs. The optimal conditions of separation and detection were achieved on a C-18 analytical column with a gradient mobile phase consisting of acetonitrile and 0.015% phosphoric acid at the flow rate of 1.0 mL min(-1) and detection wavelength set at 230 nm. All calibration curves showed good linear regression (r > 0.9995) within test ranges. This method provided good reproducibility with overall intra-and inter-day precision of less than 5% and 4% and good accuracy with recovery of more than 93%, respectively. The method was successfully applied to determine 71 samples of red and white peony roots collected from different areas. The results indicated that the contents of eight compounds varied significantly among the samples determined, which mainly resulted from processing procedure and habitat variation. The roots of Paeonio veitchii Lynch. contained a much higher amount of gallic acid and pentagalloylglucose than that of Poeonia lactiflora Pall., which could be used to distinguish the two similar species.

语种英语
WOS记录号WOS:000234235400005
引用统计
被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63366
专题北京大学药学院
作者单位1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China
3.Hebei Med Univ, Sch Pharm, Shijiazhuang 050017, Peoples R China
推荐引用方式
GB/T 7714
Wang, Q,Liu, RX,Guo, HZ,et al. Simultaneous LC determination of major constituents in red and white peony root[J]. CHROMATOGRAPHIA,2005,62(11-12):581-588.
APA Wang, Q.,Liu, RX.,Guo, HZ.,Ye, M.,Huo, CH.,...&Guo, D.(2005).Simultaneous LC determination of major constituents in red and white peony root.CHROMATOGRAPHIA,62(11-12),581-588.
MLA Wang, Q,et al."Simultaneous LC determination of major constituents in red and white peony root".CHROMATOGRAPHIA 62.11-12(2005):581-588.
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