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学科主题: 药学
题名:
Simultaneous LC determination of major constituents in red and white peony root
作者: Wang, Q; Liu, RX; Guo, HZ; Ye, M; Huo, CH; Bi, KS; Guo, D
关键词: column liquid chromatography ; red and white peony roots ; Paeonia lactiflora Pall ; Paeonia veitchii Lynch
刊名: CHROMATOGRAPHIA
发表日期: 2005-12-01
DOI: 10.1365/s10337-005-0660-2
卷: 62, 期:11-12, 页:581-588
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: PAEONIAE RADIX ; QUANTITATIVE-ANALYSIS ; MOUTAN CORTEX ; CRUDE DRUGS ; PAEONIFLORIN ; CHROMATOGRAPHY ; INHIBITION ; ALBIFLORIN
英文摘要:

A reversed phase high performance liquid chromatography method was established for the simultaneous determination of eight major constituents, namely gallic acid, paeoniflorin sulfonate, catechin, albiflorin, paeoniflorin, benzoic acid, pentagalloylglucose and benzoylpaeoniflorin in red and white peony roots, the two commonly used traditional Chinese medicinal herbs. The optimal conditions of separation and detection were achieved on a C-18 analytical column with a gradient mobile phase consisting of acetonitrile and 0.015% phosphoric acid at the flow rate of 1.0 mL min(-1) and detection wavelength set at 230 nm. All calibration curves showed good linear regression (r > 0.9995) within test ranges. This method provided good reproducibility with overall intra-and inter-day precision of less than 5% and 4% and good accuracy with recovery of more than 93%, respectively. The method was successfully applied to determine 71 samples of red and white peony roots collected from different areas. The results indicated that the contents of eight compounds varied significantly among the samples determined, which mainly resulted from processing procedure and habitat variation. The roots of Paeonio veitchii Lynch. contained a much higher amount of gallic acid and pentagalloylglucose than that of Poeonia lactiflora Pall., which could be used to distinguish the two similar species.

语种: 英语
WOS记录号: WOS:000234235400005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63366
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China
3.Hebei Med Univ, Sch Pharm, Shijiazhuang 050017, Peoples R China

Recommended Citation:
Wang, Q,Liu, RX,Guo, HZ,et al. Simultaneous LC determination of major constituents in red and white peony root[J]. CHROMATOGRAPHIA,2005,62(11-12):581-588.
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