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学科主题口腔医学
E-selectin expression induced by Porphyromonas gingivalis in human endothelial cells via nucleotide-binding oligomerization domain-like receptors and Toll-like receptors
Wan, M.1; Liu, J. R.1; Wu, D.1,2; Chi, X. P.1,3; Ouyang, X. Y.1
关键词endothelial cell nucleotide-binding oligomerization domain proteins Porphyromonas gingivalis Toll-like receptors
刊名MOLECULAR ORAL MICROBIOLOGY
2015-10-01
DOI10.1111/omi.12102
30期:5页:399-410
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine ; Microbiology
资助者National Natural Science Foundation of China ; Ministry of Science and Technology of China under International Science &amp ; Technology Cooperation Program Foundation ; National Natural Science Foundation of China ; Ministry of Science and Technology of China under International Science &amp ; Technology Cooperation Program Foundation
研究领域[WOS]Dentistry, Oral Surgery & Medicine ; Microbiology
关键词[WOS]INTRACELLULAR ADHESION MOLECULE-1 ; NOD-LIKE RECEPTORS ; MAPK PATHWAY ; P38 MAPK ; ACTIVATION ; LIPOPOLYSACCHARIDE ; FIBROBLASTS ; DYSFUNCTION ; PATHOGENS ; DISEASE
英文摘要

Porphyromonas gingivalis, an important periodontal pathogen, has been proved to actively invade cells, induce endothelial cell activation, and promote development of atherosclerosis. Innate immune surveillance, which includes the activity of nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) and Toll-like receptors (TLRs), are essential for the control of microbial infections; however, the roles of receptor families in P.gingivalis infections remain unclear. Here, we examined the roles of NLRs and TLRs in endothelial cell activation caused by P.gingivalis. Live P.gingivalis and whole cell sonicates were used to stimulate endothelial cells, and both showed upregulation of E-selectin as well as NOD1, NOD2, and TLR2. In addition, silencing of these genes in endothelial cells infected with P.gingivalis led to a reduction in E-selectin expression. Porphyromonas gingivalis also induced nuclear factor-B (NF-B) and P38 mitogen-activated protein kinase (MAPK) activity in endothelial cells, whereas small interfering RNA targeting NOD1 significantly reduced these signals. Moreover, inhibition of either NOD2 or TLR2 inhibited NF-B significantly, but had only a weak inhibitory effect on P38 MAPK signaling. Direct inhibition of NF-B and P38 MAPK significantly attenuated E-selectin expression induced by P.gingivalis in endothelial cells. Taken together, these findings suggest that NOD1, NOD2, and TLR2 play important, non-redundant roles in endothelial cell activation following P.gingivalis infection.

语种英语
所属项目编号81070840 ; 1019
资助者National Natural Science Foundation of China ; Ministry of Science and Technology of China under International Science &amp ; Technology Cooperation Program Foundation ; National Natural Science Foundation of China ; Ministry of Science and Technology of China under International Science &amp ; Technology Cooperation Program Foundation
WOS记录号WOS:000361007200006
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63369
Collection北京大学口腔医学院_牙周科
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100081, Peoples R China
2.Capital Med Univ, Bijing Anzhen Hosp, Dept Stomatol, Beijing, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept VIP Dent Serv, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Wan, M.,Liu, J. R.,Wu, D.,et al. E-selectin expression induced by Porphyromonas gingivalis in human endothelial cells via nucleotide-binding oligomerization domain-like receptors and Toll-like receptors[J]. MOLECULAR ORAL MICROBIOLOGY,2015,30(5):399-410.
APA Wan, M.,Liu, J. R.,Wu, D.,Chi, X. P.,&Ouyang, X. Y..(2015).E-selectin expression induced by Porphyromonas gingivalis in human endothelial cells via nucleotide-binding oligomerization domain-like receptors and Toll-like receptors.MOLECULAR ORAL MICROBIOLOGY,30(5),399-410.
MLA Wan, M.,et al."E-selectin expression induced by Porphyromonas gingivalis in human endothelial cells via nucleotide-binding oligomerization domain-like receptors and Toll-like receptors".MOLECULAR ORAL MICROBIOLOGY 30.5(2015):399-410.
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