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Runx2 overexpression enhances osteoblastic differentiation and mineralization in adipose - derived stem cells in vitro and in vivo
Zhang, X.; Yang, M.; Lin, L.; Chen, P.; Ma, K. T.; Zhou, C. Y.; Ao, Y. F.
关键词adipose-derived stem cells Runx2 gene therapy differentiation osteogenesis
刊名CALCIFIED TISSUE INTERNATIONAL
2006-09-01
DOI10.1007/s00223-006-0083-6
79期:3页:169-178
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Endocrinology & Metabolism
研究领域[WOS]Endocrinology & Metabolism
关键词[WOS]BONE MORPHOGENETIC PROTEIN-2 ; GENE-THERAPY ; TRANSCRIPTION FACTOR ; STROMAL CELLS ; TISSUE ; REGENERATION ; RUNX2/CBFA1 ; EXPRESSION ; MODEL
英文摘要

Like bone marrow stromal cells, adipose tissue-derived stem cells (ADSCs) possess multilineage potential, a capacity for self-renewal and long-term viability. To confirm whether ADSCs represent a promising source of cells for gene-enhanced bone tissue-engineering, the osteogenic potential of ADSCs under the control of certain osteoinductive genes has been evaluated. Runx2, a transcription factor at the downstream end of bone morphogenetic protein (BMP) signaling pathways, is essential for osteoblast differentiation and bone formation. In this study we used adenovirus vector to deliver Runx2 to ADSCs and then examined the enhancement of osteogenic activity. Overexpression of Runx2 inhibited adipogenesis, as demonstrated by suppression of LPL and PPAR gamma expression at the mRNA level and reduced lipid droplet formation. Moreover, ADSCs transduced with AdRunx2 underwent rapid and marked osteoblast differentiation as determined by osteoblastic gene expression, alkaline phosphatase activity and mineral deposition. Additionally, histological examination revealed that implantation of Runx2 modified ADSCs could induce mineral deposition and bone-like tissue formation in vivo. These results confirmed, firstly, the ability of Runx2 to promote osteogenesis and cell differentiation and, secondly, the competence of ADSCs as target cells for bone tissue engineering. Our work demonstrates a potential new approach for bone repair using Runx2-modified ADSCs for bone tissue engineering.

语种英语
WOS记录号WOS:000240713600006
引用统计
被引频次:79[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63376
专题基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
2.Wannan Med Coll, Yijishan Hosp, Dept Orthoped, Wuhu 241001, Peoples R China
3.Peking Univ, Hosp 3, Dept Sports Med, Beijing 100083, Peoples R China
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GB/T 7714
Zhang, X.,Yang, M.,Lin, L.,et al. Runx2 overexpression enhances osteoblastic differentiation and mineralization in adipose - derived stem cells in vitro and in vivo[J]. CALCIFIED TISSUE INTERNATIONAL,2006,79(3):169-178.
APA Zhang, X..,Yang, M..,Lin, L..,Chen, P..,Ma, K. T..,...&Ao, Y. F..(2006).Runx2 overexpression enhances osteoblastic differentiation and mineralization in adipose - derived stem cells in vitro and in vivo.CALCIFIED TISSUE INTERNATIONAL,79(3),169-178.
MLA Zhang, X.,et al."Runx2 overexpression enhances osteoblastic differentiation and mineralization in adipose - derived stem cells in vitro and in vivo".CALCIFIED TISSUE INTERNATIONAL 79.3(2006):169-178.
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