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学科主题临床医学
IL10, IL11, IL18 are differently expressed in CD14(+) TAMs and play different role in regulating the invasion of gastric cancer cells under hypoxia
Shen, Zhanlong1,2; Seppanen, Hanna2; Vainionpaa, Sanna2; Ye, Yingjiang1; Wang, Shan1; Mustonen, Harri2; Puolakkainen, Pauli2
关键词Interleukin-10 (IL10) Interleukin-11 (IL11) Interleukin-18 (IL18) Tumor associated macrophages (TAMs) Gastric cancer
刊名CYTOKINE
2012-08-01
DOI10.1016/j.cyto.2012.04.033
59期:2页:352-357
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology ; Immunology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology ; Immunology
关键词[WOS]TUMOR-ASSOCIATED MACROPHAGES ; NF-KAPPA-B ; SERUM INTERLEUKIN-18 ; ISCHEMIC TISSUES ; LUNG-CANCER ; PROGRESSION ; CARCINOMA ; INFLAMMATION ; MECHANISMS ; POLARIZATION
英文摘要

Background: Recent evidence shows that chronic inflammation mediated by tumor-associated macrophage (TAM) play an important role in malignant tumor formation and progression. Interleukins expressed in TAMs regulate this progress. Hypoxia is a salient feature of solid tumors and has a profound influence on the biology of TAMs However, the role of interleukins in the gastric cancer progression under hypoxia is not clear.

Methods: Realtime RT-PCR was used to quantitatively investigate the IL10, IL11 and IL18 expression in CD14(-) normal macrophages and CD14(+) TAMs co-cultured with four gastric cancer cell lines including non-metastatic cell line AGS and metastatic cell lines HGC-27, Hs-746T and NCI-N87 under normal or hypoxic conditions. In addition, the correlation between IL10, IL11, IL18 expression in TAMs under hypoxia and mobility of gastric cancer cells were analyzed.

Results: Under normal conditions, the 110 and IL18 expressions were significantly higher in CD14(+) TAMs co-cultured with non-metastatic cell line than with metastatic cell lines. IL11 expression was significantly higher in CD14(+) TAMs co-cultured with distant metastasis cell lines. Hypoxia induced IL10, IL11 and IL18 expression up regulated significantly in TAMs co-cultured with AGS, Hs-746T and NCI-N87 cell line. There was a significant negative correlation between IL11 expression in CD14(+) TAMs and gastric cancer cell invasion speed under hypoxic conditions (r = 0.861, P < 0.001).

Conclusion: The up-regulation of MO, IL11 and IL18 expression in TAMs by hypoxia differed in gastric cancer cell lines. IL11 expression in TAMs might play more important role than IL10 and IL18 expression in regulating the invasion of gastric cancer cells under hypoxia. (C) 2012 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000306159200025
资助机构Sigrid Juselius Foundation ; Helsinki University Central Hospital Research Funds
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63394
专题北京大学第二临床医学院_胃肠外科
作者单位1.Peking Univ Peoples Hosp, Dept Surg Gastroenterol, Beijing 100044, Peoples R China
2.Univ Helsinki, Cent Hosp, Dept Surg, Helsinki 00290, Finland
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GB/T 7714
Shen, Zhanlong,Seppanen, Hanna,Vainionpaa, Sanna,et al. IL10, IL11, IL18 are differently expressed in CD14(+) TAMs and play different role in regulating the invasion of gastric cancer cells under hypoxia[J]. CYTOKINE,2012,59(2):352-357.
APA Shen, Zhanlong.,Seppanen, Hanna.,Vainionpaa, Sanna.,Ye, Yingjiang.,Wang, Shan.,...&Puolakkainen, Pauli.(2012).IL10, IL11, IL18 are differently expressed in CD14(+) TAMs and play different role in regulating the invasion of gastric cancer cells under hypoxia.CYTOKINE,59(2),352-357.
MLA Shen, Zhanlong,et al."IL10, IL11, IL18 are differently expressed in CD14(+) TAMs and play different role in regulating the invasion of gastric cancer cells under hypoxia".CYTOKINE 59.2(2012):352-357.
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