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Amino acid sequence analysis in patients with chronic HCV genotype 1b infection during pegylated interferon-alpha 2a and ribavirin therapy
Fu, Juan-Juan1,2; Kong, Wei-Jing1,2; Jiang, Dong2; Pan, Xiu-Cheng1; Wei, Lai2
刊名ANTIVIRAL THERAPY
2014
DOI10.3851/IMP2759
19期:7页:661-668
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Infectious Diseases ; Pharmacology & Pharmacy ; Virology
研究领域[WOS]Infectious Diseases ; Pharmacology & Pharmacy ; Virology
关键词[WOS]HEPATITIS-C VIRUS ; DEPENDENT RNA-POLYMERASE ; PLUS RIBAVIRIN ; COMBINATION THERAPY ; CRYSTAL-STRUCTURE ; MUTATIONS ; REGION ; NS5B ; SENSITIVITY ; CORRELATE
英文摘要

Background: Amino acid variations in several HCV genomic regions have been reported to be associated with response to interferon (IFN)-alpha plus ribavirin (RBV) combination therapy. However, the results remain controversial. In this study, we further investigated the amino acid variation of full-length HCV genome and its correlation to the response to pegylated interferon (PEG-IFN)-alpha 2a and RBV combination therapy in patients with HCV genotype 1b (HCV-1b).

Methods: We retrospectively analysed 18 chronic HCV-1b patients (9 with rapid virological response and 9 non-response to therapy) treated with PEG-IFN-alpha 2a plus RBV for 48 weeks. The nearly full-length HCV genome sequence was amplified by reverse transcription (RT)-PCR followed by cloning and sequencing. Genetic diversity differences between two groups were analysed including the number of amino acid variations in the HCV polyprotein and the mean pair-wise protein distance.

Results: No single amino acid variations were closely associated with treatment outcome. However, the number of amino acid mutations in the NS5B region especially in the thumb domain and in the NS5A-V3 region was associated with the response to PEG-IFN-alpha/RBV therapy (P=0.002 and P=0.029, respectively). The number of substitutions in the NS5B region was significantly correlated with the numbers of substitutions in the V3 region (r=0.568, P=0.027).

Conclusions: Amino acid substitutions in the NS5B region especially in the thumb domain and the NS5A-V3 region may play a role in the response to combined PEG-IFN-a2a and RBV therapy in HCV-1b patients.

语种英语
WOS记录号WOS:000348603400005
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63410
专题北京大学第二临床医学院
北京大学第二临床医学院_北京大学肝病研究所
北京大学第三临床医学院_运动医学研究所
作者单位1.Xuzhou Med Coll, Affiliated Hosp, Dept Infect Dis, Xuzhou, Peoples R China
2.Peking Univ, Peoples Hosp, Hepatol Inst, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Fu, Juan-Juan,Kong, Wei-Jing,Jiang, Dong,et al. Amino acid sequence analysis in patients with chronic HCV genotype 1b infection during pegylated interferon-alpha 2a and ribavirin therapy[J]. ANTIVIRAL THERAPY,2014,19(7):661-668.
APA Fu, Juan-Juan,Kong, Wei-Jing,Jiang, Dong,Pan, Xiu-Cheng,&Wei, Lai.(2014).Amino acid sequence analysis in patients with chronic HCV genotype 1b infection during pegylated interferon-alpha 2a and ribavirin therapy.ANTIVIRAL THERAPY,19(7),661-668.
MLA Fu, Juan-Juan,et al."Amino acid sequence analysis in patients with chronic HCV genotype 1b infection during pegylated interferon-alpha 2a and ribavirin therapy".ANTIVIRAL THERAPY 19.7(2014):661-668.
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