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Ionic Extraction of a Novel Nano-sized Bioactive Glass Enhances Differentiation and Mineralization of Human Dental Pulp Cells
Gong, Weiyu1; Huang, Zhiwei1; Dong, Yanmei1; Gan, Yehua2; Li, Shenglin2; Gao, Xuejun1; Chen, Xiaofeng3
关键词Differentiation human dental pulp cells mineralization nano-sized bioactive glass
刊名JOURNAL OF ENDODONTICS
2014
DOI10.1016/j.joen.2013.08.018
40期:1页:83-88
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]IN-VITRO ; TRIOXIDE AGGREGATE ; STEM-CELLS ; CALCIUM HYDROXIDE ; CAPPING MATERIAL ; TISSUE ; DENTINOGENESIS ; REPAIR ; BONE ; CYTODIFFERENTIATION
英文摘要

Introduction: This study aimed to investigate the effects of a novel nano-sized 585 bioactive glass (nano-58S BG) on the odontogenic differentiation and mineralization of human dental pulp cells (hDPCs) in vitro. Methods: Extractions were prepared by incubating nano-58S BG, 4555 BG, or 585 BG particulates in Dulbecco modified Eagle medium at 1% w/v for 24 hours and were filtrated through 0.22-mu m filters. The supernatants were used as BG extractions. The hDPCs were cultured in nano-58S BG, 45S5 BG, and 585 BG extractions. The proliferation of hDPCs was evaluated using the methylthiazol tetrazolium assay. Odontogenic differentiation was evaluated based on the real-time polymerase chain reaction of differentiation- and mineralization-related genes, namely, alkaline phosphatase.(ALP), collagen type I, dentin sialophosphoprotein (DSPP), and dentin matrix protein 1. The gene expressions were verified using ALP activity assessment, immunocytochemistry staining of osteocalcin and DSPP, and mineralization assay using alizarin red S stain. Results: All BG extractions up-regulated the expression of odontogenic genes, and the most significant enhancement was in the nano-58S BG group. All BG extractions, especially nano-58S, increased ALP activity, osteocalcin and DSPP protein production, and mineralized nodules formation. Conclusions: Compared with regular BG, the novel nano-585 BG can induce the differentiation and mineralization of hDPCs more efficiently and might be a better potential candidate for dentin-pulp complex regeneration.

语种英语
所属项目编号51372005 ; 50830101
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000329413900014
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63439
专题北京大学口腔医学院
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Cariol & Endodontol, Beijing 100871, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100871, Peoples R China
3.S China Univ Technol, Natl Engn Res Ctr Human Tissue Restorat & Reconst, Guangzhou, Guangdong, Peoples R China
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GB/T 7714
Gong, Weiyu,Huang, Zhiwei,Dong, Yanmei,et al. Ionic Extraction of a Novel Nano-sized Bioactive Glass Enhances Differentiation and Mineralization of Human Dental Pulp Cells[J]. JOURNAL OF ENDODONTICS,2014,40(1):83-88.
APA Gong, Weiyu.,Huang, Zhiwei.,Dong, Yanmei.,Gan, Yehua.,Li, Shenglin.,...&Chen, Xiaofeng.(2014).Ionic Extraction of a Novel Nano-sized Bioactive Glass Enhances Differentiation and Mineralization of Human Dental Pulp Cells.JOURNAL OF ENDODONTICS,40(1),83-88.
MLA Gong, Weiyu,et al."Ionic Extraction of a Novel Nano-sized Bioactive Glass Enhances Differentiation and Mineralization of Human Dental Pulp Cells".JOURNAL OF ENDODONTICS 40.1(2014):83-88.
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