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学科主题: 口腔医学
题名:
Ionic Extraction of a Novel Nano-sized Bioactive Glass Enhances Differentiation and Mineralization of Human Dental Pulp Cells
作者: Gong, Weiyu1; Huang, Zhiwei1; Dong, Yanmei1; Gan, Yehua2; Li, Shenglin2; Gao, Xuejun1; Chen, Xiaofeng3
关键词: Differentiation ; human dental pulp cells ; mineralization ; nano-sized bioactive glass
刊名: JOURNAL OF ENDODONTICS
发表日期: 2014
DOI: 10.1016/j.joen.2013.08.018
卷: 40, 期:1, 页:83-88
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: IN-VITRO ; TRIOXIDE AGGREGATE ; STEM-CELLS ; CALCIUM HYDROXIDE ; CAPPING MATERIAL ; TISSUE ; DENTINOGENESIS ; REPAIR ; BONE ; CYTODIFFERENTIATION
英文摘要:

Introduction: This study aimed to investigate the effects of a novel nano-sized 585 bioactive glass (nano-58S BG) on the odontogenic differentiation and mineralization of human dental pulp cells (hDPCs) in vitro. Methods: Extractions were prepared by incubating nano-58S BG, 4555 BG, or 585 BG particulates in Dulbecco modified Eagle medium at 1% w/v for 24 hours and were filtrated through 0.22-mu m filters. The supernatants were used as BG extractions. The hDPCs were cultured in nano-58S BG, 45S5 BG, and 585 BG extractions. The proliferation of hDPCs was evaluated using the methylthiazol tetrazolium assay. Odontogenic differentiation was evaluated based on the real-time polymerase chain reaction of differentiation- and mineralization-related genes, namely, alkaline phosphatase.(ALP), collagen type I, dentin sialophosphoprotein (DSPP), and dentin matrix protein 1. The gene expressions were verified using ALP activity assessment, immunocytochemistry staining of osteocalcin and DSPP, and mineralization assay using alizarin red S stain. Results: All BG extractions up-regulated the expression of odontogenic genes, and the most significant enhancement was in the nano-58S BG group. All BG extractions, especially nano-58S, increased ALP activity, osteocalcin and DSPP protein production, and mineralized nodules formation. Conclusions: Compared with regular BG, the novel nano-585 BG can induce the differentiation and mineralization of hDPCs more efficiently and might be a better potential candidate for dentin-pulp complex regeneration.

语种: 英语
所属项目编号: 51372005 ; 50830101
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000329413900014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63439
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Cariol & Endodontol, Beijing 100871, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100871, Peoples R China
3.S China Univ Technol, Natl Engn Res Ctr Human Tissue Restorat & Reconst, Guangzhou, Guangdong, Peoples R China

Recommended Citation:
Gong, Weiyu,Huang, Zhiwei,Dong, Yanmei,et al. Ionic Extraction of a Novel Nano-sized Bioactive Glass Enhances Differentiation and Mineralization of Human Dental Pulp Cells[J]. JOURNAL OF ENDODONTICS,2014,40(1):83-88.
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