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Transplantation of granulocytic myeloid-derived suppressor cells (G-MDSCs) could reduce colitis in experimental murine models
Su, Hui1; Cong, Xu2; Liu, Yu Lan1
关键词CD11b+Gr-1+cells immune regulation inflammatory bowel disease myeloid-derived suppressor cell
刊名JOURNAL OF DIGESTIVE DISEASES
2013-05-01
DOI10.1111/1751-2980.12029
14期:5页:251-258
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]INFLAMMATORY-BOWEL-DISEASE ; TNBS-INDUCED COLITIS ; IMMUNE
英文摘要

Objective Myeloid-derived suppressor cells (MDSCs) are a group of myeloid cells expressing CD11b and Gr-1 marker in mice and comprise at least two subsets: granulocytic MDSCs (G-MDSCs) and monocytic MDSCs (M-MDSCs). This study aimed to evaluate the therapeutic efficacy of transplantation of G-MDSC subsets from normal mice to colitis mice. Methods Murine colitis model was induced by the intrarectal administration of 2,4,6-trinitrobenzenesulfonic acid (TNBS). The mice were divided into four groups: control group, TNBS-induced colitis, TNBS-induced colitis plus normal saline injection and TNBS-induced colitis plus bone marrow-derived G-MDSCs injection (transplantation group). G-MDSCs were sorted and enriched via magnetic-activated cell sorting (MACS) program, the purity of the sorted cells was then identified using flow cytometry analysis. Sex cross-transplantation of dominant G-MDSCs was applied from normal mice to colitis models using i.v. injection. Changes of body weight, survival rate, myeloperoxidase (MPO) activity were monitored and macroscopic and microscopic injury scores are calculated. Donor cell Y chromosomes were assessed by in situ hybridization to assess reconstitutions. Results After the transplantation of bone marrow-derived G-MDSCs from normal mice to colitis models, recipient mice showed increased survival rate, decreased macroscopic and microscopic injury scores and MPO activity, as well as lowered concentration of serum interleukin-6. Y chromosomes staining displayed colonization of donor cells of liver, spleen and colon tissues. Conclusion Bone marrow-derived G-MDSCs are effective in the improvement of murine colitis, but its effect in human needs further investigation.

语种英语
WOS记录号WOS:000317895100006
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63454
专题北京大学第二临床医学院_消化科
作者单位1.Peking Univ, Peoples Hosp, Dept Gastroenterol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
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Su, Hui,Cong, Xu,Liu, Yu Lan. Transplantation of granulocytic myeloid-derived suppressor cells (G-MDSCs) could reduce colitis in experimental murine models[J]. JOURNAL OF DIGESTIVE DISEASES,2013,14(5):251-258.
APA Su, Hui,Cong, Xu,&Liu, Yu Lan.(2013).Transplantation of granulocytic myeloid-derived suppressor cells (G-MDSCs) could reduce colitis in experimental murine models.JOURNAL OF DIGESTIVE DISEASES,14(5),251-258.
MLA Su, Hui,et al."Transplantation of granulocytic myeloid-derived suppressor cells (G-MDSCs) could reduce colitis in experimental murine models".JOURNAL OF DIGESTIVE DISEASES 14.5(2013):251-258.
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