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学科主题: 临床医学
题名:
Overexpression of Thymosin Beta-10 Inhibits VEGF mRNA Expression, Autocrine VEGF Protein Production, and Tube Formation in Hypoxia-Induced Monkey Choroid-Retinal Endothelial Cells
作者: Zhang, Tonghe1; Li, Xiaoxin1; Yu, Wenzhen1; Yan, Zheng2; Zou, He3; He, Xiangjun2
关键词: Thymosin Beta-10 ; Hypoxia ; Adenovirus vector ; Angiogenesis ; Tube formation
刊名: OPHTHALMIC RESEARCH
发表日期: 2009
DOI: 10.1159/000163220
卷: 41, 期:1, 页:36-43
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: EPITHELIUM-DERIVED FACTOR ; GROWTH-FACTOR ; KAPPA-B ; NEOVASCULARIZATION ; ANGIOGENESIS ; MODEL ; RETINOPATHY ; ACTIVATION ; MIGRATION ; PEPTIDES
英文摘要:

Purpose: One purpose of this study was to examine if expression of the thymosin beta(10) (TB10), thymosin beta(4) (TB4), and VEGF165 isoform genes would change in response to hypoxia in monkey choroidal retinal endothelial cells (RF/6A). We also sought to determine if overexpression of the TB10 gene could inhibit VEGF165 mRNA expression, autocrine VEGF production, and tube formation in hypoxic RF/6A cells. Methods: An adenovirus vector, Ad-TB10, was constructed to deliver the human TB10 gene. Under hypoxic conditions, TB10, TB4, and VEGF165 mRNA were measured using real-time PCR, and VEGF protein expression was evaluated by ELISA. For tube formation assays, the RF/6A cells were transfected with Ad-TB10 and exposed to hypoxia for 4 h. Results: VEGF165 mRNA was 2- and 5.8-fold increased when the RF/6A cells were exposed to hypoxia for 2 and 4 h, respectively. TB10 mRNA decreased at 2 h and increased to a baseline level at 4 h, while TB4 mRNA level increased slightly by 2 and 4 h. Increased VEGF165 mRNA was down-regulated by transfected Ad-TB10, and ELISA results showed that VEGF protein levels increased 2.4-fold after 4-hour hypoxia, and decreased 2-fold after transfection with Ad-TB10. Hypoxia also induced tube formation of the RF/6A cells. This was significantly reduced by Ad-TB10. Conclusion: These data indicate that (1) TB4 and TB10 mRNA expression in RF/6A cells present different changes under 2- and 4-hour hypoxia, and (2) TB10 may be an effective inhibitor of angiogenesis by inhibiting VEGF expression and autocrine protein production. Copyright (C) 2008 S. Karger AG, Basel

语种: 英语
所属项目编号: 2005CB724307
项目资助者: National Basic Research Program of China
WOS记录号: WOS:000260891700006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63480
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Ctr Lab, Beijing 100044, Peoples R China
3.Jilin Univ, Second Hosp, Dept Ophthalmol, Changchun 130023, Peoples R China

Recommended Citation:
Zhang, Tonghe,Li, Xiaoxin,Yu, Wenzhen,et al. Overexpression of Thymosin Beta-10 Inhibits VEGF mRNA Expression, Autocrine VEGF Protein Production, and Tube Formation in Hypoxia-Induced Monkey Choroid-Retinal Endothelial Cells[J]. OPHTHALMIC RESEARCH,2009,41(1):36-43.
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