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学科主题: 药学
题名:
Effective and Reversible DNA Condensation Induced by a Simple Cyclic/Rigid Polyamine Containing Carbonyl Moiety
作者: Li, Chao1; Ma, Chunying1; Xu, Pengxiang3; Gao, Yuxing3; Zhang, Jin1; Qiao, Renzhong1,2; Zhao, Yufen3
刊名: JOURNAL OF PHYSICAL CHEMISTRY B
发表日期: 2013-07-04
DOI: 10.1021/jp312766u
卷: 117, 期:26, 页:7857-7867
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Physical
研究领域[WOS]: Chemistry
关键词[WOS]: CATIONIC GEMINI AMPHIPHILES ; COUNTERION CONDENSATION ; CHARGE INVERSION ; GENE DELIVERY ; TRANSFECTION ; COMPLEX ; NANOPARTICLES ; LIPOPLEXES ; DENDRIMERS ; MOLECULE
英文摘要:

The transfection of DNA in gene therapy largely depends on the possibility of obtaining its condensation. The details of nanoparticle formation are essential for functioning, as mediated by the diverse elements containing molecular structure, ionic strength in mediums, and condensing motivator. Here, we report two kinds of DNA condensing agents based on simple cyclic/rigid polyamine molecules, having evaluated their structural effect on nanoparticle formation. The reversible condensation-dissociation process was achieved by ion switching, attributing to a possible condensing mechanism-competitive building of external hydrogen bonds. Using poly[(dA-dT)(2)] and poly[(dG-dC)(2)] as substrates, respectively, circular dichroism (CD) signals clearly presented dissimilar interactions between polyamines and both rich sequences, implying potential preference for G-C sequence. The presence of divalent ion Zn2+ as an efficient motivator accelerated the achievement of DNA condensation, and an accessible schematic model was depicted to explain the promotion in detail. In addition, by comparison with the behaviors of linear polyamines, differences between condensation and aggregation were explicitly elucidated in aspects of morphology and surface charges, as well as induced condition. The present work may have the potential to reveal the precise mechanism of DNA nanoparticle formation and, in particular, be applied to gene delivery as an efficient nonviral vector.

语种: 英语
所属项目编号: 20972014 ; 21202005 ; 21232005 ; 21172016
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000321542200002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63486
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
3.Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China

Recommended Citation:
Li, Chao,Ma, Chunying,Xu, Pengxiang,et al. Effective and Reversible DNA Condensation Induced by a Simple Cyclic/Rigid Polyamine Containing Carbonyl Moiety[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2013,117(26):7857-7867.
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