IR@PKUHSC  > 北京大学药学院
学科主题药学
Effective and Reversible DNA Condensation Induced by a Simple Cyclic/Rigid Polyamine Containing Carbonyl Moiety
Li, Chao1; Ma, Chunying1; Xu, Pengxiang3; Gao, Yuxing3; Zhang, Jin1; Qiao, Renzhong1,2; Zhao, Yufen3
刊名JOURNAL OF PHYSICAL CHEMISTRY B
2013-07-04
DOI10.1021/jp312766u
117期:26页:7857-7867
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Physical
研究领域[WOS]Chemistry
关键词[WOS]CATIONIC GEMINI AMPHIPHILES ; COUNTERION CONDENSATION ; CHARGE INVERSION ; GENE DELIVERY ; TRANSFECTION ; COMPLEX ; NANOPARTICLES ; LIPOPLEXES ; DENDRIMERS ; MOLECULE
英文摘要

The transfection of DNA in gene therapy largely depends on the possibility of obtaining its condensation. The details of nanoparticle formation are essential for functioning, as mediated by the diverse elements containing molecular structure, ionic strength in mediums, and condensing motivator. Here, we report two kinds of DNA condensing agents based on simple cyclic/rigid polyamine molecules, having evaluated their structural effect on nanoparticle formation. The reversible condensation-dissociation process was achieved by ion switching, attributing to a possible condensing mechanism-competitive building of external hydrogen bonds. Using poly[(dA-dT)(2)] and poly[(dG-dC)(2)] as substrates, respectively, circular dichroism (CD) signals clearly presented dissimilar interactions between polyamines and both rich sequences, implying potential preference for G-C sequence. The presence of divalent ion Zn2+ as an efficient motivator accelerated the achievement of DNA condensation, and an accessible schematic model was depicted to explain the promotion in detail. In addition, by comparison with the behaviors of linear polyamines, differences between condensation and aggregation were explicitly elucidated in aspects of morphology and surface charges, as well as induced condition. The present work may have the potential to reveal the precise mechanism of DNA nanoparticle formation and, in particular, be applied to gene delivery as an efficient nonviral vector.

语种英语
WOS记录号WOS:000321542200002
项目编号20972014 ; 21202005 ; 21232005 ; 21172016
资助机构National Nature Science Foundation of China
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63486
专题北京大学药学院
北京大学口腔医学院_材料检测中心
作者单位1.Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
3.Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China
推荐引用方式
GB/T 7714
Li, Chao,Ma, Chunying,Xu, Pengxiang,et al. Effective and Reversible DNA Condensation Induced by a Simple Cyclic/Rigid Polyamine Containing Carbonyl Moiety[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2013,117(26):7857-7867.
APA Li, Chao.,Ma, Chunying.,Xu, Pengxiang.,Gao, Yuxing.,Zhang, Jin.,...&Zhao, Yufen.(2013).Effective and Reversible DNA Condensation Induced by a Simple Cyclic/Rigid Polyamine Containing Carbonyl Moiety.JOURNAL OF PHYSICAL CHEMISTRY B,117(26),7857-7867.
MLA Li, Chao,et al."Effective and Reversible DNA Condensation Induced by a Simple Cyclic/Rigid Polyamine Containing Carbonyl Moiety".JOURNAL OF PHYSICAL CHEMISTRY B 117.26(2013):7857-7867.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Chao]的文章
[Ma, Chunying]的文章
[Xu, Pengxiang]的文章
百度学术
百度学术中相似的文章
[Li, Chao]的文章
[Ma, Chunying]的文章
[Xu, Pengxiang]的文章
必应学术
必应学术中相似的文章
[Li, Chao]的文章
[Ma, Chunying]的文章
[Xu, Pengxiang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。