IR@PKUHSC  > 北京大学第二临床医学院  > 胃肠外科
学科主题临床医学
Vasohibin-1 and vasohibin-2 expression in gastric cancer cells and TAMs
Shen, Zhanlong1,2; Kauttu, Tuuli2; Seppanen, Hanna2; Vainionpaa, Sanna2; Ye, Yingjiang1; Wang, Shan1; Mustonen, Harri2; Puolakkainen, Pauli2
关键词Vasohibin-1 Vasohibin-2 Tumor-associated macrophages (TAMs) Gastric cancer Angiogenesis
刊名MEDICAL ONCOLOGY
2012-12-01
DOI10.1007/s12032-012-0212-1
29期:4页:2718-2726
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]TUMOR-ASSOCIATED MACROPHAGES ; ANGIOGENESIS INHIBITOR ; ENDOTHELIUM ; GROWTH ; VEGF ; INFLAMMATION ; CARCINOMA ; FEEDBACK ; BREAST
英文摘要

Accumulating evidence suggests that TAMs contribute to tumor progression. Recently, vasohibin-1 and vasohibin-2 were detected in endothelial cells and considered as intrinsic angiogenesis inhibitors. However, it is not known whether they are also expressed in cancer cells or tumor-associated macrophages (TAMs). Realtime RT-PCR was used to investigate the vasohibin-1 and vasohibin-2 expression in four gastric cancer cell lines, including a non-metastatic cell line AGS, and metastatic cell lines HGC-27, Hs-746T and NCI-N87, co-cultured with or without TAMs. The effect of hypoxic conditions on vasohibin expression was evaluated as well, and the correlation between vasohibin-1, vasohibin-2 and VEGF-A expression under different culture conditions was analyzed. We found that both vasohibin-1 and vasohibin-2 were expressed in the four gastric cancer cell lines and in TAMs. Under normal conditions, vasohibin-1 and vasohibin-2 expressions were significantly upregulated by TAMs in all the gastric cancer cell lines. Under hypoxia, both vasohibin-1 and vasohibin-2 expressions were significantly decreased in the distant metastasis cancer cell line Hs-746T, cultured with or without TAMs (P < 0.001). After induction by TAMs or hypoxia, the vasohibin-1 and vasohibin-2 expressions correlated with that of VEGF-A. In addition, TAMs, when co-cultured with the metastatic cancer cell lines, showed hypoxia-induced vasohibin-1 upregulation (P < 0.05). In conclusion, both vasohibin-1 and vasohibin-2 mRNA are expressed in gastric cancer cells and in TAMs, and their expressions are altered by hypoxia.

语种英语
WOS记录号WOS:000309711600063
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63487
Collection北京大学第二临床医学院_胃肠外科
作者单位1.Peking Univ, Dept Surg Gastroenterol, Peoples Hosp, Beijing 100044, Peoples R China
2.Univ Helsinki, Helsinki Univ Cent Hosp, Dept Surg, Helsinki 00290, Finland
Recommended Citation
GB/T 7714
Shen, Zhanlong,Kauttu, Tuuli,Seppanen, Hanna,et al. Vasohibin-1 and vasohibin-2 expression in gastric cancer cells and TAMs[J]. MEDICAL ONCOLOGY,2012,29(4):2718-2726.
APA Shen, Zhanlong.,Kauttu, Tuuli.,Seppanen, Hanna.,Vainionpaa, Sanna.,Ye, Yingjiang.,...&Puolakkainen, Pauli.(2012).Vasohibin-1 and vasohibin-2 expression in gastric cancer cells and TAMs.MEDICAL ONCOLOGY,29(4),2718-2726.
MLA Shen, Zhanlong,et al."Vasohibin-1 and vasohibin-2 expression in gastric cancer cells and TAMs".MEDICAL ONCOLOGY 29.4(2012):2718-2726.
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