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GM1 up-regulates ubiquilin 1 expression in human neuroblastoma cells and rat cortical neurons
Liu, Zhonghua; Ruan, Yanyan; Yue, Weihua; Zhu, Zhihui; Hartmann, Tobias; Beyreuther, Konrad; Zhang, Dai
关键词ubiquilin 1 GM I ganglioside Alzheimer&prime s disease gene expression suppression subtractive hybridization
刊名NEUROSCIENCE LETTERS
2006-10-16
DOI10.1016/j.neulet.2006.08.005
407期:1页:59-63
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]AMYLOID PRECURSOR PROTEIN ; ALZHEIMERS-DISEASE ; BETA-PROTEIN ; GROWTH-FACTOR ; BRAIN ; INCREASES ; ACCUMULATION ; PROTEOLYSIS ; PEPTIDE
英文摘要

GM1 ganglioside was reported to mediate the amyloid beta-protein (A beta) secretion and accumulation in the pathogenesis of Alzheimer′s disease (AD). The objective of this project was to comprehend the underlying molecular changes related to amyloid beta-protein precursor (APP) processing pathway induced by GM1. Using suppression subtractive hybridisation (SSH), we detected one prominent sequence with increased expression in human neuroblastoma cells that stably transfected with human APP695 cDNA treated with GM1. This transcript has high identity to human Ubiquilin 1 gene. Differential expression was initially confirmed by dot blot hybridization. This result was further authenticated with quantitative real-time polymerase chain reaction (RT-PCR) analysis. Furthermore, using Western blots, we discovered that GM1 stimulated the expression of Ubiquilin 1 in human neuroblastoma cells and rat cortical neurons while other gangliosides Asialo-GM1 and GD1b did not. Ubiquilin 1 is one of the candidate genes of AD, which have been shown to modulate the gamma-secretase components in the proteolytic processing of APP, and is therefore a putative candidate for further investigation of GM1 mechanisms in the etiology and pathology of AD. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000241122200012
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63492
专题北京大学精神卫生研究所
作者单位1.Peking Univ, Inst Mental Hlth, Dept Biochem, Beijing 100871, Peoples R China
2.Univ Heidelberg, ZMBH Ctr Mol Biol Heidelberg, D-6900 Heidelberg, Germany
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Liu, Zhonghua,Ruan, Yanyan,Yue, Weihua,et al. GM1 up-regulates ubiquilin 1 expression in human neuroblastoma cells and rat cortical neurons[J]. NEUROSCIENCE LETTERS,2006,407(1):59-63.
APA Liu, Zhonghua.,Ruan, Yanyan.,Yue, Weihua.,Zhu, Zhihui.,Hartmann, Tobias.,...&Zhang, Dai.(2006).GM1 up-regulates ubiquilin 1 expression in human neuroblastoma cells and rat cortical neurons.NEUROSCIENCE LETTERS,407(1),59-63.
MLA Liu, Zhonghua,et al."GM1 up-regulates ubiquilin 1 expression in human neuroblastoma cells and rat cortical neurons".NEUROSCIENCE LETTERS 407.1(2006):59-63.
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