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学科主题: 精神卫生
题名:
GM1 up-regulates ubiquilin 1 expression in human neuroblastoma cells and rat cortical neurons
作者: Liu, Zhonghua; Ruan, Yanyan; Yue, Weihua; Zhu, Zhihui; Hartmann, Tobias; Beyreuther, Konrad; Zhang, Dai
关键词: ubiquilin 1 ; GM I ganglioside ; Alzheimer&prime ; s disease ; gene expression ; suppression subtractive hybridization
刊名: NEUROSCIENCE LETTERS
发表日期: 2006-10-16
DOI: 10.1016/j.neulet.2006.08.005
卷: 407, 期:1, 页:59-63
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: AMYLOID PRECURSOR PROTEIN ; ALZHEIMERS-DISEASE ; BETA-PROTEIN ; GROWTH-FACTOR ; BRAIN ; INCREASES ; ACCUMULATION ; PROTEOLYSIS ; PEPTIDE
英文摘要:

GM1 ganglioside was reported to mediate the amyloid beta-protein (A beta) secretion and accumulation in the pathogenesis of Alzheimer′s disease (AD). The objective of this project was to comprehend the underlying molecular changes related to amyloid beta-protein precursor (APP) processing pathway induced by GM1. Using suppression subtractive hybridisation (SSH), we detected one prominent sequence with increased expression in human neuroblastoma cells that stably transfected with human APP695 cDNA treated with GM1. This transcript has high identity to human Ubiquilin 1 gene. Differential expression was initially confirmed by dot blot hybridization. This result was further authenticated with quantitative real-time polymerase chain reaction (RT-PCR) analysis. Furthermore, using Western blots, we discovered that GM1 stimulated the expression of Ubiquilin 1 in human neuroblastoma cells and rat cortical neurons while other gangliosides Asialo-GM1 and GD1b did not. Ubiquilin 1 is one of the candidate genes of AD, which have been shown to modulate the gamma-secretase components in the proteolytic processing of APP, and is therefore a putative candidate for further investigation of GM1 mechanisms in the etiology and pathology of AD. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000241122200012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63492
Appears in Collections:北京大学精神卫生研究所_期刊论文

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作者单位: 1.Peking Univ, Inst Mental Hlth, Dept Biochem, Beijing 100871, Peoples R China
2.Univ Heidelberg, ZMBH Ctr Mol Biol Heidelberg, D-6900 Heidelberg, Germany

Recommended Citation:
Liu, Zhonghua,Ruan, Yanyan,Yue, Weihua,et al. GM1 up-regulates ubiquilin 1 expression in human neuroblastoma cells and rat cortical neurons[J]. NEUROSCIENCE LETTERS,2006,407(1):59-63.
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