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学科主题: 药学
题名:
Synthesis, physicochemical and biological properties of oligonucleotides incorporated with amino-isonucleosides
作者: Wang Fang; Chen Yue; Huang Ye; Jin Hong-Wei; Zhang Liang-Ren; Yang Zhen-Jun; Zhang Li-He
关键词: amino-isonucleoside ; antisense oligonucleotide ; siRNA
刊名: SCIENCE CHINA-CHEMISTRY
发表日期: 2012
DOI: 10.1007/s11426-011-4465-x
卷: 55, 期:1, 页:70-79
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: RNA INTERFERENCE ; MAMMALIAN-CELLS ; SIRNA ; EXPRESSION ; STABILITY ; DELIVERY ; ENZYME ; STRAND
英文摘要:

Antisense oligonucleotides (ASONs) and siRNAs have been applied extensively for the regulation of cellular and viral gene expression, and RNAi is currently one of the most promising new approaches for anti-tumor and anti-viral therapy. In order to improve bioactivity properties and physicochemical properties of siRNA, we synthesized a novel class of ASONs II-VII incorporated with amino-isonucleoside (isoA(1) and isoA(2)) for investigation on basic physicochemical properties. Then we designed amino-isonucleoside (isoA(1), isoA(2) and isoT(1)) incorporated siRNA 2-7. Some meaningful results have been obtained from the physicochemical property experiments in ASONs. In RNAi potency experiments, we investigated RNAi potency of each strand of the siRNA. These amino-isonucleosides incorporated siRNAs showed promising bioactivity properties and had position specificity. Reduced off target effect from sense strand loading in siRNA application was observed.

语种: 英语
所属项目编号: 20932001 ; 2006AA02Z144 ; 2009ZX09503
项目资助者: National Natural Science Foundation of China ; Ministry of Science and Technology of China
WOS记录号: WOS:000298968200010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63493
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China

Recommended Citation:
Wang Fang,Chen Yue,Huang Ye,et al. Synthesis, physicochemical and biological properties of oligonucleotides incorporated with amino-isonucleosides[J]. SCIENCE CHINA-CHEMISTRY,2012,55(1):70-79.
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