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Synthesis, physicochemical and biological properties of oligonucleotides incorporated with amino-isonucleosides
Wang Fang; Chen Yue; Huang Ye; Jin Hong-Wei; Zhang Liang-Ren; Yang Zhen-Jun; Zhang Li-He
关键词amino-isonucleoside antisense oligonucleotide siRNA
刊名SCIENCE CHINA-CHEMISTRY
2012
DOI10.1007/s11426-011-4465-x
55期:1页:70-79
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]RNA INTERFERENCE ; MAMMALIAN-CELLS ; SIRNA ; EXPRESSION ; STABILITY ; DELIVERY ; ENZYME ; STRAND
英文摘要

Antisense oligonucleotides (ASONs) and siRNAs have been applied extensively for the regulation of cellular and viral gene expression, and RNAi is currently one of the most promising new approaches for anti-tumor and anti-viral therapy. In order to improve bioactivity properties and physicochemical properties of siRNA, we synthesized a novel class of ASONs II-VII incorporated with amino-isonucleoside (isoA(1) and isoA(2)) for investigation on basic physicochemical properties. Then we designed amino-isonucleoside (isoA(1), isoA(2) and isoT(1)) incorporated siRNA 2-7. Some meaningful results have been obtained from the physicochemical property experiments in ASONs. In RNAi potency experiments, we investigated RNAi potency of each strand of the siRNA. These amino-isonucleosides incorporated siRNAs showed promising bioactivity properties and had position specificity. Reduced off target effect from sense strand loading in siRNA application was observed.

语种英语
WOS记录号WOS:000298968200010
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63493
专题北京大学药学院
北京大学医学部管理机构_后勤处
北京大学药学院_天然药物与仿生药物国家重点实验室
北京大学药学院_药物化学系
作者单位Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
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GB/T 7714
Wang Fang,Chen Yue,Huang Ye,et al. Synthesis, physicochemical and biological properties of oligonucleotides incorporated with amino-isonucleosides[J]. SCIENCE CHINA-CHEMISTRY,2012,55(1):70-79.
APA Wang Fang.,Chen Yue.,Huang Ye.,Jin Hong-Wei.,Zhang Liang-Ren.,...&Zhang Li-He.(2012).Synthesis, physicochemical and biological properties of oligonucleotides incorporated with amino-isonucleosides.SCIENCE CHINA-CHEMISTRY,55(1),70-79.
MLA Wang Fang,et al."Synthesis, physicochemical and biological properties of oligonucleotides incorporated with amino-isonucleosides".SCIENCE CHINA-CHEMISTRY 55.1(2012):70-79.
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