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Cloning of Aspergillus fumigatus histidine kinase gene fragment and its expression during invasive infection
Du, C; Li, RY; Ma, SQ; Wang, DL
关键词Aspergillus fumigatus mice gene expression histidine kinase invasive pulmonary aspergillosis
刊名MYCOPATHOLOGIA
2002
153期:1页:5-10
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Mycology
研究领域[WOS]Mycology
关键词[WOS]PULMONARY ASPERGILLOSIS ; NIDULANS ; HOMOLOG ; SYSTEM ; FUNGUS ; LUNG ; MICE
英文摘要

It was demonstrated recently that three histidine kinases genes in Candida albicans contributed to virulence, indicating the importance of signaling pathways regulated by histidine kinases. In the present study, using a set of degenerate primers, RT-PCR was performed with cDNA of A. fumigatus as a template. PCR products were cloned and sequenced. After Blast analysis, it was found that one fragment (named as AFHK1). 305 bp, was highly homologous to the two-component histidine kinase tesA gene of Aspergillus nidulans. But AFHK1 was not completely identical to the FOS-1 gene of A. fumigatus. The same A. fumigatus strain was used to inoculate the mice for a murine model of invasive pulmonary aspergillosis (IPA). After 5-days post-inoculation, the lungs of infected animals were removed and incubated for 2 h at 37 degreesC in digestion buffer containing collagenase and trypsin. The pulmonary cells were removed by passing the suspension through a sieve. The non-filterable hyphae were treated with deoxygenated sodium cholate. Total RNA of A. fumigatus isolated from the infected tissues or cultured in vitro was extracted. With AFHK1 as a probe, a Northern blot was performed. A 3.0 kb (approximate) transcript of mRNA was detected corresponding to the putative histidine kinase gene. It was demonstrated that that gene was expressed at markedly higher levels in vivo than in vitro. The results suggest that this gene may contribute to the survival and virulence of A. fumigatus.

语种英语
WOS记录号WOS:000174308600002
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63498
专题北京大学第一临床医学院_皮肤性病科
作者单位Peking Univ, Hosp 1, Res Ctr Med Mycol, Dept Dermatol, Beijing 100871, Peoples R China
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Du, C,Li, RY,Ma, SQ,et al. Cloning of Aspergillus fumigatus histidine kinase gene fragment and its expression during invasive infection[J]. MYCOPATHOLOGIA,2002,153(1):5-10.
APA Du, C,Li, RY,Ma, SQ,&Wang, DL.(2002).Cloning of Aspergillus fumigatus histidine kinase gene fragment and its expression during invasive infection.MYCOPATHOLOGIA,153(1),5-10.
MLA Du, C,et al."Cloning of Aspergillus fumigatus histidine kinase gene fragment and its expression during invasive infection".MYCOPATHOLOGIA 153.1(2002):5-10.
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