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学科主题: 药学
题名:
Green tea polysaccharide-conjugates protect human umbilical vein endothelial cells against impairments triggered by high glucose
作者: Chen, Xiaoqiang1; Wang, Yuefei2; Wu, Yalin3; Han, Baoyu4; Zhu, Yuejin1; Tang, Xiaolin1; Sun, Qinglei1
关键词: Green tea ; Polysaccharide-conjugates ; Diabetes ; Vascular complications ; High glucose ; ROS scavenging
刊名: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
发表日期: 2011-07-01
DOI: 10.1016/j.ijbiomac.2011.03.008
卷: 49, 期:1, 页:50-54
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: WATER-SOLUBLE POLYSACCHARIDE ; COMPLICATIONS ; DYSFUNCTION ; DISEASE ; ASSAY
英文摘要:

Hot-water extracts of low-grade green tea were precipitated with ethanol, deproteinized with trichloroacetic acid, neutralized with NaOH and fractionated by DEAE-cellulose DE-52 column chromatography to yield three (3) of unexplored polysaccharide-conjugate fractions termed gTPC1, gTPC2 and gTPC3. Monosaccharide and amino acid composition, contents of total neutral sugars, proteins and moistures, HPGPC distribution and Zeta potentials of gTPC1-3 were investigated. Exposure of human umbilical vein endothelial (HUVE) cells to high glucose (33 mM) for 12 h significantly decreased cell viability relative to normal glucose control (p < 0.001). As compared with cell injury group, gTPC1-3 at all of three dose levels (50,150 and 300 mu g/mL) were found to possess remarkably protective effects on HUVE cells against impairments induced by high glucose in a dose-dependent manner (p < 0.05, p < 0.001). To contribute toward our understanding of the cell-based protection mechanism of gTPC1-3, the latter were subjected to self-oxidation of 1,2,3-phentriol assay, and their scavenging effects were observed as 55.1%, 47.6% and 47.9% at the concentration of 300 mu g/mL, respectively. On the basis of the fact that high glucose-induced endothelial dysfunction involves in the overproduction of reactive oxygen species (ROS) and contributes to the vascular complications in patients with diabetes, inhibitory effects of gTPC1-3 on high glucose-mediated HUVE cell loss are, at least in part, correlated with their potential scavenging potency of ROS. Taken together, gTPC1-3 could be developed as non-cytotoxic candidates of therapeutic agent for diabetic vascular complications. (C) 2011 Elsevier B.V. All rights reserved.

语种: 英语
项目资助者: MU one-hundred people program
WOS记录号: WOS:000291840400008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63526
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.China COOP, Hangzhou Tea Res Inst, Hangzhou 310016, Zhejiang, Peoples R China
2.Zhejiang Univ, Dept Tea Sci, Hangzhou 310029, Zhejiang, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China
4.China Jiliang Univ, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China

Recommended Citation:
Chen, Xiaoqiang,Wang, Yuefei,Wu, Yalin,et al. Green tea polysaccharide-conjugates protect human umbilical vein endothelial cells against impairments triggered by high glucose[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2011,49(1):50-54.
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