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Alterations of ovariectomized rat bone and impact of non-collagenous proteins on mineralization
Huang, Jian1; Wang, Xi1; Zhang, Tian-Lan1,2; Wang, Kui1
关键词Non-collagenous proteins Bone mineral Ovariectomized rat Osteopenia Mineralization
刊名JOINT BONE SPINE
2009-03-01
DOI10.1016/j.jbspin.2008.04.017
76期:2页:176-183
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Rheumatology
研究领域[WOS]Rheumatology
关键词[WOS]MATRIX PROTEINS ; DEFICIENT MICE ; OSTEOBLAST DIFFERENTIATION ; IN-VITRO ; HYDROXYAPATITE ; SIALOPROTEIN ; OSTEOPONTIN ; OSTEOPOROSIS ; OSTEONECTIN ; RESORPTION
英文摘要

Objectives: Noncollagenous proteins (NCPs) play distinct and overlap roles in controlling the mineralization of bone, while the relationship between the NCPs and the mineral phase in osteoporotic bone is nebulous. Ovariectomized (OVX) rats, as an animal model for osteopenia, were used in this study to evaluate the impact of NCPs on mineral metabolism in osteopenia development.

Methods: Bone mineral was characterized with small-angle X-ray scattering study, extended X-ray absorption fine structure analysis, inductively coupled plasma mass spectroscopy, thermogravimetric and chemical analyses. NCPs were extracted from bone matrix, and analyzed with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). A constant composition titration system was used in the kinetic study of re-mineralization of bone residues after various extractions.

Results: Bone density was reduced, whereas no significant changes were observed in the composition, crystal thickness and crystal structural parameters of minerals from OVX rat bone. The total content of NCPs in each extract decreased in the OVX group, but no selective loss of particular proteins was observed. The difference in mineralization kinetics between the OVX rat bone and the sham rat bone was resulted mainly from the mineral-bound NCPs.

Conclusions: The reduced quantity and unchanged quality of bone mineral seem to be positively associated with the total amount of NCPs and the proportions between some major NCPs in OVX rats, respectively. (C) 2008 Elsevier Masson SAS. All rights reserved.

语种英语
WOS记录号WOS:000264461400012
项目编号20571006 ; 2007CB516806 ; 2062007
资助机构National Natural Science Foundation of China ; Ministry of Science and Technology of China ; Beijing Natural Science Foundation
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63528
专题北京大学药学院_化学生物学系
北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China
2.Capital Med Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100069, Peoples R China
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GB/T 7714
Huang, Jian,Wang, Xi,Zhang, Tian-Lan,et al. Alterations of ovariectomized rat bone and impact of non-collagenous proteins on mineralization[J]. JOINT BONE SPINE,2009,76(2):176-183.
APA Huang, Jian,Wang, Xi,Zhang, Tian-Lan,&Wang, Kui.(2009).Alterations of ovariectomized rat bone and impact of non-collagenous proteins on mineralization.JOINT BONE SPINE,76(2),176-183.
MLA Huang, Jian,et al."Alterations of ovariectomized rat bone and impact of non-collagenous proteins on mineralization".JOINT BONE SPINE 76.2(2009):176-183.
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