北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 化学生物学系  > 期刊论文
学科主题: 药学
题名:
Alterations of ovariectomized rat bone and impact of non-collagenous proteins on mineralization
作者: Huang, Jian1; Wang, Xi1; Zhang, Tian-Lan1,2; Wang, Kui1
关键词: Non-collagenous proteins ; Bone mineral ; Ovariectomized rat ; Osteopenia ; Mineralization
刊名: JOINT BONE SPINE
发表日期: 2009-03-01
DOI: 10.1016/j.jbspin.2008.04.017
卷: 76, 期:2, 页:176-183
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Rheumatology
研究领域[WOS]: Rheumatology
关键词[WOS]: MATRIX PROTEINS ; DEFICIENT MICE ; OSTEOBLAST DIFFERENTIATION ; IN-VITRO ; HYDROXYAPATITE ; SIALOPROTEIN ; OSTEOPONTIN ; OSTEOPOROSIS ; OSTEONECTIN ; RESORPTION
英文摘要:

Objectives: Noncollagenous proteins (NCPs) play distinct and overlap roles in controlling the mineralization of bone, while the relationship between the NCPs and the mineral phase in osteoporotic bone is nebulous. Ovariectomized (OVX) rats, as an animal model for osteopenia, were used in this study to evaluate the impact of NCPs on mineral metabolism in osteopenia development.

Methods: Bone mineral was characterized with small-angle X-ray scattering study, extended X-ray absorption fine structure analysis, inductively coupled plasma mass spectroscopy, thermogravimetric and chemical analyses. NCPs were extracted from bone matrix, and analyzed with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). A constant composition titration system was used in the kinetic study of re-mineralization of bone residues after various extractions.

Results: Bone density was reduced, whereas no significant changes were observed in the composition, crystal thickness and crystal structural parameters of minerals from OVX rat bone. The total content of NCPs in each extract decreased in the OVX group, but no selective loss of particular proteins was observed. The difference in mineralization kinetics between the OVX rat bone and the sham rat bone was resulted mainly from the mineral-bound NCPs.

Conclusions: The reduced quantity and unchanged quality of bone mineral seem to be positively associated with the total amount of NCPs and the proportions between some major NCPs in OVX rats, respectively. (C) 2008 Elsevier Masson SAS. All rights reserved.

语种: 英语
所属项目编号: 20571006 ; 2007CB516806 ; 2062007
项目资助者: National Natural Science Foundation of China ; Ministry of Science and Technology of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000264461400012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63528
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China
2.Capital Med Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100069, Peoples R China

Recommended Citation:
Huang, Jian,Wang, Xi,Zhang, Tian-Lan,et al. Alterations of ovariectomized rat bone and impact of non-collagenous proteins on mineralization[J]. JOINT BONE SPINE,2009,76(2):176-183.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Huang, Jian]'s Articles
[Wang, Xi]'s Articles
[Zhang, Tian-Lan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Huang, Jian]‘s Articles
[Wang, Xi]‘s Articles
[Zhang, Tian-Lan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace