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学科主题: 基础医学
题名:
Cell-based Screening and Validation of Human Novel Gene TMEM9B Activated Nuclear Factor kappa B Signaling Pathway
作者: Liu Yu-Ming1,3; Peng Zhi1; Deng Wei-Wei1; Shi Tai-Ping1,2; Ma Da-Long1,2
关键词: cell-based screening ; TMEM9B ; NF-kappa B ; cell apoptosis
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2009-11-01
DOI: 10.3724/SP.J.1206.2009.00256
卷: 36, 期:11, 页:1423-1428
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: THERAPEUTIC TARGET ; DEATH ; APOPTOSIS ; DISEASES ; CANCER
英文摘要:

Nuclear factor kappa B (NF-kappa B) is an important cellular transcription factor. The important role of NF-kappa B-mediated cell signal transduction pathway in apoptosis is a hot topic at home and abroad. In order to discover new regulators In NF-kappa B signaling pathway, a high-throughput cell-based screening model based on dual luciferase reporters system was established, a number of genes that can activate NF-kappa B signal pathway were obtained by screening of 439 novel function genes. Among them, TMEM9B can obviously activate NF-kappa B signaling pathway. Further experiments showed that TMEM9B activated NF-kappa B signaling pathway in a dose-dependent pattern. Western blotting and EMSA experiments confirmed that TMEM9B can promote the degradation of I kappa B alpha (a cytoplasm inhibitor of NF-kappa B), and cause NF-kappa B shift from the cytoplasm to nucleus. At the same time, flow cytometry result demonstrated TMEM9B can induce apoptosis in HEK293T and HeLa cells. In short, a stable and effective screening system for NF-kappa B has been established, through which TMEM9B was identified to be able to significantly activate NF-kappa B signal transduction pathway and thus cause cells apoptosis.

语种: 中文
所属项目编号: 2006AA02A305
项目资助者: Hi-Tech Research and Development Program of China
WOS记录号: WOS:000272227400005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63547
Appears in Collections:基础医学院_免疫学系_期刊论文

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细胞水平筛选鉴定激活核因子_B通路的新基因TMEM9B_刘宇明.pdf(560KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Lab Med Immunol, Beijing 100191, Peoples R China
3.Inner Mongolia Univ Technol, Chem Engn Coll, Hohhot 010051, Peoples R China

Recommended Citation:
Liu Yu-Ming,Peng Zhi,Deng Wei-Wei,et al. Cell-based Screening and Validation of Human Novel Gene TMEM9B Activated Nuclear Factor kappa B Signaling Pathway[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2009,36(11):1423-1428.
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