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学科主题基础医学
Cell-based Screening and Validation of Human Novel Gene TMEM9B Activated Nuclear Factor kappa B Signaling Pathway
Liu Yu-Ming1,3; Peng Zhi1; Deng Wei-Wei1; Shi Tai-Ping1,2; Ma Da-Long1,2
关键词cell-based screening TMEM9B NF-kappa B cell apoptosis
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2009-11-01
DOI10.3724/SP.J.1206.2009.00256
36期:11页:1423-1428
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]THERAPEUTIC TARGET ; DEATH ; APOPTOSIS ; DISEASES ; CANCER
英文摘要

Nuclear factor kappa B (NF-kappa B) is an important cellular transcription factor. The important role of NF-kappa B-mediated cell signal transduction pathway in apoptosis is a hot topic at home and abroad. In order to discover new regulators In NF-kappa B signaling pathway, a high-throughput cell-based screening model based on dual luciferase reporters system was established, a number of genes that can activate NF-kappa B signal pathway were obtained by screening of 439 novel function genes. Among them, TMEM9B can obviously activate NF-kappa B signaling pathway. Further experiments showed that TMEM9B activated NF-kappa B signaling pathway in a dose-dependent pattern. Western blotting and EMSA experiments confirmed that TMEM9B can promote the degradation of I kappa B alpha (a cytoplasm inhibitor of NF-kappa B), and cause NF-kappa B shift from the cytoplasm to nucleus. At the same time, flow cytometry result demonstrated TMEM9B can induce apoptosis in HEK293T and HeLa cells. In short, a stable and effective screening system for NF-kappa B has been established, through which TMEM9B was identified to be able to significantly activate NF-kappa B signal transduction pathway and thus cause cells apoptosis.

语种中文
WOS记录号WOS:000272227400005
项目编号2006AA02A305
资助机构Hi-Tech Research and Development Program of China
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63547
专题北京大学基础医学院_免疫学系
作者单位1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Lab Med Immunol, Beijing 100191, Peoples R China
3.Inner Mongolia Univ Technol, Chem Engn Coll, Hohhot 010051, Peoples R China
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Liu Yu-Ming,Peng Zhi,Deng Wei-Wei,et al. Cell-based Screening and Validation of Human Novel Gene TMEM9B Activated Nuclear Factor kappa B Signaling Pathway[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2009,36(11):1423-1428.
APA Liu Yu-Ming,Peng Zhi,Deng Wei-Wei,Shi Tai-Ping,&Ma Da-Long.(2009).Cell-based Screening and Validation of Human Novel Gene TMEM9B Activated Nuclear Factor kappa B Signaling Pathway.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,36(11),1423-1428.
MLA Liu Yu-Ming,et al."Cell-based Screening and Validation of Human Novel Gene TMEM9B Activated Nuclear Factor kappa B Signaling Pathway".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 36.11(2009):1423-1428.
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