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学科主题基础医学
Primary functional identification of gene TMSG-1
Ma, CS; Ning, JY; You, JF; Liu, L; Wang, JL; Cui, XL; Wu, BQ; Zheng, J
关键词prostate cancer gene transfection pH apoptosis
刊名SCIENCE IN CHINA SERIES C-LIFE SCIENCES
2003-12-01
46期:6页:641-650
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biology
研究领域[WOS]Life Sciences & Biomedicine - Other Topics
关键词[WOS]INTRACELLULAR PH ; H+-ATPASE ; APOPTOSIS ; ACTIVATION ; CELLS
英文摘要

TMSG-1 was a tumor metastasis-related gene identified using mRNA differential display, whose expression level was lower in cancer cell lines with higher metastatic potential and in tumor tissue with metastasis. TMSG-1 was transfected to prostate cancer cell line (PC-3M-1 E8) with high metastatic potential to observe the effects of increased expression of TMSG-1 on V-ATPase activity, intracellular pH and cell apoptosis. Subcellular localization of the encoded protein of TMSG-1 was determined by using GFP. Results showed that there were no differences of V-ATPase activity among parental PC-3M-1E8 cell line, pcDNA3 transfectant and anti-TMSG-1 transtectant, whereas the V-ATPase activity was significantly higher in TMSG-1 transfectant than that in parental PC-3M-1 E8 cell line, pcDNA3 transfectant and Anti-TMSG-1 transfectant (p<0.001). Intracellular pH (pHi) was detected by using the pH-dependent fluorescence probe BECEF Results showed the pHi was significantly increased in TMSG-1 transfectant. Cell apoptosis assay demonstrated cell apoptosis was significantly higher in -1 transfectant (p<0.01) and BCL2 expression was down regulated. Subcellular localization of TMSG-1 protein showed TMSG-1 was a transmembrane protein, which predicted TMSG-1 protein was located in cytoplasm system, such as endoplasmic reticulum and mitochondrial. These results indicated TMSG-1 up regulation in prostate cancer cell line could promote V-ATPase activity, increase pHi and cell apoptosis, and inhibit the expression of BCL2.

语种英语
WOS记录号WOS:000186828500009
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63552
专题基础医学院_病理学系
作者单位1.Peking Univ, Hlth Sci Ctr, Anal Ctr Med & Hyg, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China
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Ma, CS,Ning, JY,You, JF,et al. Primary functional identification of gene TMSG-1[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2003,46(6):641-650.
APA Ma, CS.,Ning, JY.,You, JF.,Liu, L.,Wang, JL.,...&Zheng, J.(2003).Primary functional identification of gene TMSG-1.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,46(6),641-650.
MLA Ma, CS,et al."Primary functional identification of gene TMSG-1".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 46.6(2003):641-650.
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