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学科主题: 临床医学
题名:
Human umbilical cord mesenchymal stem cells: Osteogenesis in vivo as seed cells for bone tissue engineering
作者: Diao, Yinze1; Ma, Qingjun1; Cui, Fuzhai2; Zhong, Yanfeng3
关键词: mesenchymal stem cells ; bone tissue engineering ; umbilical cord ; heterotopic osteogenesis ; in vivo
刊名: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
发表日期: 2009-10-01
DOI: 10.1002/jbm.a.32186
卷: 91A, 期:1, 页:123-131
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: MARROW STROMAL CELLS ; COLLAGEN MATRIX ; TRANSPLANTATION ; DIFFERENTIATION ; AGE ; PROLIFERATION ; ANTIBODIES ; REPAIR ; DEFECT ; UTERO
英文摘要:

Mesenchymal stem cells (MSCs) are ideal seed cells for bone tissue engineering. However, intrinsic deficiencies exist for the autologous transplantation strategy of constructing artificial bone with MSCs derived from bone marrow of patients. In this study, MSCs-like cells were isolated from human umbilical cords and were expanded in vitro. Flow cytometric analysis revealed that cells from the fourth passage were positive for CD29, CD44, CD71, CD73, CD90, and CD105 whereas they were negative for CD14, CD34, CD45, and CD117. Furthermore, these cells expressed HLA-A, B, C (MHC-I), but not HLA-DP, DQ, DR (MHC-II), or costimulatory molecules such as CD80 and CD86. Following incubation in specific inductive media for 3 weeks, cultured cells were shown to possess potential to differentiate into adipogenic, osteogenic or chondrogenic lineages in vitro. The umbilical cord-derived MSCs (UC-MSCs) were loaded with a biomimetic artificial bone scaffold material before being implanted subcutaneously in the back of Balb/c nude mice for four to twelve weeks. Our results revealed that UC-MSCs loaded with the scaffold displayed capacity of osteogenic differentiation leading to osteogenesis with human origin in vivo. As a readily available source of seed cells for bone tissue engineering, UC-MSCs should have broad application prospects. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 91A: 123-131, 2009

语种: 英语
所属项目编号: 30571870
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000269627700014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63558
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Orthopaed Surg, Beijing 100083, Peoples R China
2.Tsinghua Univ, Dept Mat Sci & Engn, Biomat Lab, Beijing 100084, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China

Recommended Citation:
Diao, Yinze,Ma, Qingjun,Cui, Fuzhai,et al. Human umbilical cord mesenchymal stem cells: Osteogenesis in vivo as seed cells for bone tissue engineering[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2009,91A(1):123-131.
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