IR@PKUHSC  > 北京大学第三临床医学院  > 骨科
学科主题临床医学
Human umbilical cord mesenchymal stem cells: Osteogenesis in vivo as seed cells for bone tissue engineering
Diao, Yinze1; Ma, Qingjun1; Cui, Fuzhai2; Zhong, Yanfeng3
关键词mesenchymal stem cells bone tissue engineering umbilical cord heterotopic osteogenesis in vivo
刊名JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
2009-10-01
DOI10.1002/jbm.a.32186
91A期:1页:123-131
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]MARROW STROMAL CELLS ; COLLAGEN MATRIX ; TRANSPLANTATION ; DIFFERENTIATION ; AGE ; PROLIFERATION ; ANTIBODIES ; REPAIR ; DEFECT ; UTERO
英文摘要

Mesenchymal stem cells (MSCs) are ideal seed cells for bone tissue engineering. However, intrinsic deficiencies exist for the autologous transplantation strategy of constructing artificial bone with MSCs derived from bone marrow of patients. In this study, MSCs-like cells were isolated from human umbilical cords and were expanded in vitro. Flow cytometric analysis revealed that cells from the fourth passage were positive for CD29, CD44, CD71, CD73, CD90, and CD105 whereas they were negative for CD14, CD34, CD45, and CD117. Furthermore, these cells expressed HLA-A, B, C (MHC-I), but not HLA-DP, DQ, DR (MHC-II), or costimulatory molecules such as CD80 and CD86. Following incubation in specific inductive media for 3 weeks, cultured cells were shown to possess potential to differentiate into adipogenic, osteogenic or chondrogenic lineages in vitro. The umbilical cord-derived MSCs (UC-MSCs) were loaded with a biomimetic artificial bone scaffold material before being implanted subcutaneously in the back of Balb/c nude mice for four to twelve weeks. Our results revealed that UC-MSCs loaded with the scaffold displayed capacity of osteogenic differentiation leading to osteogenesis with human origin in vivo. As a readily available source of seed cells for bone tissue engineering, UC-MSCs should have broad application prospects. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 91A: 123-131, 2009

语种英语
WOS记录号WOS:000269627700014
引用统计
被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63558
专题北京大学第三临床医学院_骨科
北京大学基础医学院
作者单位1.Peking Univ, Hosp 3, Dept Orthopaed Surg, Beijing 100083, Peoples R China
2.Tsinghua Univ, Dept Mat Sci & Engn, Biomat Lab, Beijing 100084, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Diao, Yinze,Ma, Qingjun,Cui, Fuzhai,et al. Human umbilical cord mesenchymal stem cells: Osteogenesis in vivo as seed cells for bone tissue engineering[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2009,91A(1):123-131.
APA Diao, Yinze,Ma, Qingjun,Cui, Fuzhai,&Zhong, Yanfeng.(2009).Human umbilical cord mesenchymal stem cells: Osteogenesis in vivo as seed cells for bone tissue engineering.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,91A(1),123-131.
MLA Diao, Yinze,et al."Human umbilical cord mesenchymal stem cells: Osteogenesis in vivo as seed cells for bone tissue engineering".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 91A.1(2009):123-131.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Diao, Yinze]的文章
[Ma, Qingjun]的文章
[Cui, Fuzhai]的文章
百度学术
百度学术中相似的文章
[Diao, Yinze]的文章
[Ma, Qingjun]的文章
[Cui, Fuzhai]的文章
必应学术
必应学术中相似的文章
[Diao, Yinze]的文章
[Ma, Qingjun]的文章
[Cui, Fuzhai]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。