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学科主题: 口腔医学
题名:
Bi-Functionalization of a Calcium Phosphate-Coated Titanium Surface with Slow-Release Simvastatin and Metronidazole to Provide Antibacterial Activities and Pro-Osteodifferentiation Capabilities
作者: Liu, Yunsong1,2; Zhang, Xiao1; Liu, Yang3; Jin, Xiaoxiao1; Fan, Cong1; Ye, Hongqiang1; Ou, Meng′ en1; Lv, Longwei1; Wu, Gang4; Zhou, Yongsheng1,2,5
刊名: PLOS ONE
发表日期: 2014-05-20
DOI: 10.1371/journal.pone.0097741
卷: 9, 期:5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: MESENCHYMAL STEM-CELL ; IN-VITRO ; CLOSTRIDIUM-DIFFICILE ; BONE ; DIFFERENTIATION ; RESISTANCE ; IMPLANTS ; ADHESION ; REGENERATION ; MECHANISM
英文摘要:

Coating the surface of titanium implants or other bone graft substitute materials with calcium phosphate (Ca-P) crystals is an effective way to enhance the osteoconduction of the implants. Ca-P coating alone cannot confer pro-osteodifferentiation and antibacterial capabilities on implants; however, it can serve as a carrier for biological agents which could improve the performance of implants and bone substitutes. Here, we constructed a novel, bi-functional Ca-P coating with combined pro-osteodifferentiation and antibacterial capabilities. Different concentrations of metronidazole (MNZ) and simvastatin (SIM) were integrated into biomimetic Ca-P coatings on the surface of titanium disks. The biological effects of this bi-functional biomimetic coating on human bone marrow mesenchymal stem cells (hBMMSCs), human adipose derived stromal cells (hASCs), and Porphyromonas gingivalis were assessed in vitro. We observed that Ca-P coatings loaded with both SIM and MNZ display favorable release kinetics without affecting cell proliferation or attachment. In the inhibition zone test, we found that the bi-functional coating showed lasting antibacterial effects when incubated with Porphyromonas gingivalis for 2 and 4 days. Moreover, the osteodifferentiation of hBMMSCs and hASCs were increased when cultured on this bi-functional coating for 7 and 14 days. Both drugs were loaded onto the Ca-P coating at specific concentrations (10 25 M SIM; 10 22 M MNZ) to achieve optimal release kinetics. Considering the safety, stability and low cost of SIM and MNZ, this novel bifunctional Ca-P coating technique represents a promising method to improve the performance of metal implants or other bone substitute materials, and can theoretically be easily translated to clinical applications.

语种: 英语
所属项目编号: 30901693 ; 81170937 ; BMU20110274
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Ministry of Education
WOS记录号: WOS:000339563400054
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63561
Appears in Collections:北京大学口腔医学院_口腔修复科_期刊论文

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作者单位: 1.Natl Engn Lab Digital & Mat Technol Stomatol, Beijing, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100871, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept Orthodont, Beijing 100871, Peoples R China
4.Vrije Univ Amsterdam, Res Inst MOVE, Acad Ctr Dentristry Amsterdam ACTA, Dept Oral Implantol & Prosthet Dent, Amsterdam, Netherlands
5.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100871, Peoples R China

Recommended Citation:
Liu, Yunsong,Zhang, Xiao,Liu, Yang,et al. Bi-Functionalization of a Calcium Phosphate-Coated Titanium Surface with Slow-Release Simvastatin and Metronidazole to Provide Antibacterial Activities and Pro-Osteodifferentiation Capabilities[J]. PLOS ONE,2014,9(5).
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