IR@PKUHSC  > 北京大学药学院
学科主题药学
A strategy for efficient discovery of new natural compounds by integrating orthogonal column chromatography and liquid chromatography/mass spectrometry analysis: Its application in Panax ginseng, Panax quinquefolium and Panax notoginseng to characterize 437 potential new ginsenosides
Yang, Wen-zhi1; Ye, Min1; Qiao, Xue1; Liu, Chun-fang1; Miao, Wen-juan1; Bo, Tao2; Tao, Hai-yan1; Guo, De-an1
关键词Natural compounds discovery Ginsenoside Panax species Orthogonal chromatography Liquid chromatography coupled with mass spectrometry
刊名ANALYTICA CHIMICA ACTA
2012-08-20
DOI10.1016/j.aca.2012.06.017
739页:56-66
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]TANDEM MASS-SPECTROMETRY ; DAMMARANE-TYPE SAPONINS ; FLOWER-BUDS ; BIOACTIVE SAPONINS ; MEDICINAL FLOWERS ; TRITERPENE OLIGOGLYCOSIDES ; AMERICAN GINSENG ; IDENTIFICATION ; ROOTS ; GLYCOSIDES
英文摘要

To discover new natural compounds from herbal medicines tends to be more and more difficult. In this paper, a strategy integrating orthogonal column chromatography and liquid chromatography/mass spectrometry (LC/MS) analysis was proposed, and was applied for rapid discovery of new ginsenosides from Panax ginseng (PG), Panax quinquefolium (PQ), and Panax notoginseng (PN). The ginsenosides extracts were fractionated by MCl gel x silica gel orthogonal column chromatography. The fractions were then separated on a C-18 HPLC column, eluted with a three-component mobile phase (CH3CN/CH3OH/3 mM CH3COONH4-H2O), and detected by electrospray ionization tandem mass spectrometry. The structures of unknown ginsenosides were elucidated by analyzing negative and positive ion mass spectra, which provided complementary information on the sapogenins and oligosaccharide chains, respectively. A total of 623 comprising 437 potential new ginsenosides were characterized from the ethanol extracts of PG, PQ and PN. New acylations, diversified saccharide chains and C-17 side chains constituted novelty of the newly identified ginsenosides. An interpretation guideline was proposed for structural characterization of unknown ginsenosides by LC/MS. To confirm reliability of this strategy, two targeted unknown trace ginsenosides were obtained in pure form by LC/MS-guided isolation. Based on extensive NMR spectroscopic analysis and other techniques, they were identified as 3-O-[6-O-(E)-butenoyl-beta-D-glucopyranosyl(1,2)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol-20-O-beta-D-glucopyranosyl(1.6)-beta-D-glucopyranoside (named ginsenoside IV) and 3-O-beta-D-glucopyranosyl(1,2)-beta-D-glucopyranosyl-3 beta,12 beta,20(S),24(R)-tetra hydroxy-dammar-25-ene-20-O-beta-D-glucopyranosyl(1,6)-beta-D-glucopyranoside (ginsenoside V), respectively. The fully established structures were consistent with the MS-oriented structural elucidation. This study expanded our understanding on ginsenosides of Panax species, and the proposed strategy was proved efficient and reliable in the discovery of new minor compounds from herbal extracts. (C) 2012 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000307139700007
引用统计
被引频次:72[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63564
专题北京大学药学院
北京大学医学部管理机构_医学部
北京大学药学院_天然药物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Agilent Technol, Beijing 100102, Peoples R China
推荐引用方式
GB/T 7714
Yang, Wen-zhi,Ye, Min,Qiao, Xue,et al. A strategy for efficient discovery of new natural compounds by integrating orthogonal column chromatography and liquid chromatography/mass spectrometry analysis: Its application in Panax ginseng, Panax quinquefolium and Panax notoginseng to characterize 437 potential new ginsenosides[J]. ANALYTICA CHIMICA ACTA,2012,739:56-66.
APA Yang, Wen-zhi.,Ye, Min.,Qiao, Xue.,Liu, Chun-fang.,Miao, Wen-juan.,...&Guo, De-an.(2012).A strategy for efficient discovery of new natural compounds by integrating orthogonal column chromatography and liquid chromatography/mass spectrometry analysis: Its application in Panax ginseng, Panax quinquefolium and Panax notoginseng to characterize 437 potential new ginsenosides.ANALYTICA CHIMICA ACTA,739,56-66.
MLA Yang, Wen-zhi,et al."A strategy for efficient discovery of new natural compounds by integrating orthogonal column chromatography and liquid chromatography/mass spectrometry analysis: Its application in Panax ginseng, Panax quinquefolium and Panax notoginseng to characterize 437 potential new ginsenosides".ANALYTICA CHIMICA ACTA 739(2012):56-66.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, Wen-zhi]的文章
[Ye, Min]的文章
[Qiao, Xue]的文章
百度学术
百度学术中相似的文章
[Yang, Wen-zhi]的文章
[Ye, Min]的文章
[Qiao, Xue]的文章
必应学术
必应学术中相似的文章
[Yang, Wen-zhi]的文章
[Ye, Min]的文章
[Qiao, Xue]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。