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学科主题: 临床医学
题名:
Optimization of dual effects of Mg-1Ca alloys on the behavior of chondrocytes and osteoblasts in vitro
作者: Dou, Yana1; Mujeeb, Ayeesha2; Zheng, Yufeng1,2; Ge, Zigang1,3,4,5
关键词: Magnesium ; Degradation ; Chondrocyte ; Phenotype
刊名: PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL
发表日期: 2014-10-01
DOI: 10.1016/j.pnsc.2014.08.001
卷: 24, 期:5, 页:433-440
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]: Materials Science ; Science & Technology - Other Topics
关键词[WOS]: BONE SUBSTITUTE ; CARTILAGE ; REGENERATION ; SCAFFOLD ; DEFECTS ; CELLS
英文摘要:

Mg ions can enhance the proliferation and redifferentiation of chondrocytes and the osteogenic differentiation of osteoblasts at specific concentrations, respectively. However, degradation of Mg alloys at varying degradation rates could lead to complex changes in the surrounding tissue environment, such as changes in the dynamic concentration of Mg ions and subsequent pH value. Considering the above mentioned factors, the comprehensive effects of Mg alloys on chondrocytes and osteoblasts behaviors have not yet been optimized In this study, we evaluated the effects of Mg-1Ca microspheres on cell behavior with an aim to optimize conditions favorable for both cell types. Cells were cultured with Mg-1Ca microspheres prepared using the following concentrations: 250 mu g/ml, 500 mu g/ml and 1000 mu g/ml. At specific time points, cytotoxicity, expression of specific genes and extracellular matrix deposition by cells (Alizarin Red Staining of osteoblasts and Alcian blue staining for chondrocytes) were evaluated. The experimental results revealed that Mg-1Ca microspheres prepared at a concentration of 250 mu g/ml were optimum for both cell types, where chondrocytes were found to be in hypertrophy state while osteoblasts in close proximity to the microspheres showed osteogenetic differentiation. Interestingly, a slight change in osteoblasts behavior was observed nearer to and at a relative distance away from Mg-1Ca microspheres, an important observation for administering the application of microspheres as potential scaffolds. (C) 2014 Chinese Materials Research Society. Production and hosting by Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2012CB619100 ; 81271722
项目资助者: National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China
WOS记录号: WOS:000346008500004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63568
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomed Mat & Tissue Engn, Beijing 100871, Peoples R China
2.Peking Univ, Coll Engn, Dept Mat Sci & Engn, Beijing 100871, Peoples R China
3.Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China
4.Peking Univ, Peoples Hosp, Arthrit Clic, Beijing 100871, Peoples R China
5.Peking Univ, Peoples Hosp, Res Ctr, Beijing 100871, Peoples R China

Recommended Citation:
Dou, Yana,Mujeeb, Ayeesha,Zheng, Yufeng,et al. Optimization of dual effects of Mg-1Ca alloys on the behavior of chondrocytes and osteoblasts in vitro[J]. PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL,2014,24(5):433-440.
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