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IR@PKUHSC  > 北京大学临床肿瘤学院  > 期刊论文
学科主题: 临床医学
题名:
Integration of DNA Copy Number Alterations and Transcriptional Expression Analysis in Human Gastric Cancer
作者: Fan, Biao1,2; Dachrut, Somkid1,3; Coral, Ho1; Yuen, Siu Tsan4; Chu, Kent Man5; Law, Simon5; Zhang, Lianhai2; Ji, Jiafu2; Leung, Suet Yi4; Chen, Xin1
刊名: PLOS ONE
发表日期: 2012-04-23
DOI: 10.1371/journal.pone.0029824
卷: 7, 期:4
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: COMPARATIVE GENOMIC HYBRIDIZATION ; GENE-EXPRESSION ; BREAST-CANCER ; HEPATOCELLULAR-CARCINOMA ; PANCREATIC-CANCER ; PROTEIN EXPRESSION ; PROSTATE-CANCER ; CELL-LINES ; AMPLIFICATION ; MYC
英文摘要:

Background: Genomic instability with frequent DNA copy number alterations is one of the key hallmarks of carcinogenesis. The chromosomal regions with frequent DNA copy number gain and loss in human gastric cancer are still poorly defined. It remains unknown how the DNA copy number variations contributes to the changes of gene expression profiles, especially on the global level.

Principal Findings: We analyzed DNA copy number alterations in 64 human gastric cancer samples and 8 gastric cancer cell lines using bacterial artificial chromosome (BAC) arrays based comparative genomic hybridization (aCGH). Statistical analysis was applied to correlate previously published gene expression data obtained from cDNA microarrays with corresponding DNA copy number variation data to identify candidate oncogenes and tumor suppressor genes. We found that gastric cancer samples showed recurrent DNA copy number variations, including gains at 5p, 8q, 20p, 20q, and losses at 4q, 9p, 18q, 21q. The most frequent regions of amplification were 20q12 (7/72), 20q12-20q13.1 (12/72), 20q13.1-20q13.2 (11/72) and 20q13.2-20q13.3 (6/72). The most frequent deleted region was 9p21 (8/72). Correlating gene expression array data with aCGH identified 321 candidate oncogenes, which were overexpressed and showed frequent DNA copy number gains; and 12 candidate tumor suppressor genes which were down-regulated and showed frequent DNA copy number losses in human gastric cancers. Three networks of significantly expressed genes in gastric cancer samples were identified by ingenuity pathway analysis.

Conclusions: This study provides insight into DNA copy number variations and their contribution to altered gene expression profiles during human gastric cancer development. It provides novel candidate driver oncogenes or tumor suppressor genes for human gastric cancer, useful pathway maps for the future understanding of the molecular pathogenesis of this malignancy, and the construction of new therapeutic targets.

语种: 英语
所属项目编号: 2010601079
项目资助者: University of California Cancer Research Coordinate Committee ; China Scholarship Council
WOS记录号: WOS:000305341000002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63569
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Univ Calif San Francisco, Dept Bioengn & Therapeut Sci, San Francisco, CA 94143 USA
2.Peking Univ, Sch Oncol, Key Lab Carcinogenesis & Translat Res, Beijing Canc Hosp & Inst,Dept Surg,Minist Educ, Beijing 100871, Peoples R China
3.Khon Kaen Univ, Fac Med, Liver Fluke & Cholangiocarcinoma Res Ctr, Khon Kaen, Thailand
4.Univ Hong Kong, Queen Mary Hosp, Dept Pathol, Pokfulam, Hong Kong, Peoples R China
5.Univ Hong Kong, Queen Mary Hosp, Dept Surg, Pokfulam, Hong Kong, Peoples R China

Recommended Citation:
Fan, Biao,Dachrut, Somkid,Coral, Ho,et al. Integration of DNA Copy Number Alterations and Transcriptional Expression Analysis in Human Gastric Cancer[J]. PLOS ONE,2012,7(4).
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