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学科主题: 基础医学
题名:
Total salvianolic acid improves ischemia-reperfusion-induced microcirculatory disturbance in rat mesentery
作者: Wang, Ming-Xia2,3; Liu, Yu-Ying2; Hu, Bai-He2; Wei, Xiao-Hong2; Chang, Xin2; Sun, Kai2; Fan, Jing-Yu2; Liao, Fu-Long2; Wang, Chuan-She1,2; Zheng, Jun2,3; Han, Jing-Yan1,2
关键词: Salvia miltiorrhiza ; Leukocyte adherence ; Oxygen-free radicals ; Albumin leakage ; Ischemia-reperfusion
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2010-11-14
DOI: 10.3748/wjg.v16.i42.5306
卷: 16, 期:42, 页:5306-5316
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: OXYGEN-FREE-RADICALS ; MAST-CELLS ; CARDIOTONIC PILLS ; ENDOTHELIAL ADHESION ; LIPID-PEROXIDATION ; CEREBRAL-ISCHEMIA ; NADPH OXIDASE ; LACTIC-ACID ; B PROTECTS ; INJURY
英文摘要:

AIM: To investigate the effect of total salvianolic acid (TSA) on ischemia-reperfusion (I/R)-induced rat mesenteric microcirculatory dysfunctions.

METHODS: Male Wistar rats were randomly distributed into 5 groups (n = 6 each): Sham group and I/R group (infused with saline), TSA group, TSA + I/R group and I/R + TSA group (infused with TSA, 5 mg/kg per hour). Mesenteric I/R were conducted by a ligation of the mesenteric artery and vein (10 min) and subsequent release of the occlusion. TSA was continuously infused either starting from 10 min before the ischemia or 10 min after reperfusion. Changes in mesenteric microcirculatory variables, including diameter of venule, velocity of red blood cells in venule, leukocyte adhesion, free radicals released from venule, albumin leakage and mast cell degranulation, were observed through an inverted intravital microscope. Meanwhile, the expression of adhesion molecules CD11b/CD18 on neutrophils was evaluated by flow cytometry. Ultrastructural evidence of mesenteric venules damage was assessed after microcirculation observation.

RESULTS: I/R led to multiple responses in mesenteric post-capillary venules, including a significant increase in the adhesion of leukocytes, production of oxygen radicals in the venular wall, albumin efflux and enhanced mast cell degranulation in vivo. All the I/R-induced manifestations were significantly reduced by pre- or post-treatment with TSA, with the exception that the I/R-induced increase in mast cell degranulation was inhibited only by pre-treatment with TSA. Moreover, pre- or post-treatment with TSA significantly attenuated the expression of CD11b/CD18 on neutrophils, reducing the increase in the number of caveolae in the endothelial cells of mesentery post-capillary venules induced by I/R.

CONCLUSION: The results demonstrated that TSA protects from and ameliorates the microcirculation disturbance induced by I/R, which was associated with TSA inhibiting the production of oxygen-free radicals in the venular wall and the expression of CD11b/CD18 on neutrophils. (C) 2010 Baishideng. All rights reserved.

语种: 英语
所属项目编号: 2008ZX09401
项目资助者: Ministry of Science and Technology of the People&prime ; s Republic of China
WOS记录号: WOS:000284494400006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63575
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Dept Integrat Chinese & Western Med, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Peking Univ, Tasly Microcirculat Res Ctr, Hlth Sci Ctr, Beijing 100191, Peoples R China
3.Tianjin Univ Tradit Chinese Med, Dept Pharmacol, Tianjin 300193, Peoples R China

Recommended Citation:
Wang, Ming-Xia,Liu, Yu-Ying,Hu, Bai-He,et al. Total salvianolic acid improves ischemia-reperfusion-induced microcirculatory disturbance in rat mesentery[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2010,16(42):5306-5316.
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