Institutional Repository of Peking University School of Basic Medical Sciences
|Total salvianolic acid improves ischemia-reperfusion-induced microcirculatory disturbance in rat mesentery|
|Wang, Ming-Xia2,3; Liu, Yu-Ying2; Hu, Bai-He2; Wei, Xiao-Hong2; Chang, Xin2; Sun, Kai2; Fan, Jing-Yu2; Liao, Fu-Long2; Wang, Chuan-She1,2; Zheng, Jun2,3; Han, Jing-Yan1,2|
|关键词||Salvia miltiorrhiza Leukocyte adherence Oxygen-free radicals Albumin leakage Ischemia-reperfusion|
|刊名||WORLD JOURNAL OF GASTROENTEROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||OXYGEN-FREE-RADICALS ; MAST-CELLS ; CARDIOTONIC PILLS ; ENDOTHELIAL ADHESION ; LIPID-PEROXIDATION ; CEREBRAL-ISCHEMIA ; NADPH OXIDASE ; LACTIC-ACID ; B PROTECTS ; INJURY|
AIM: To investigate the effect of total salvianolic acid (TSA) on ischemia-reperfusion (I/R)-induced rat mesenteric microcirculatory dysfunctions.
METHODS: Male Wistar rats were randomly distributed into 5 groups (n = 6 each): Sham group and I/R group (infused with saline), TSA group, TSA + I/R group and I/R + TSA group (infused with TSA, 5 mg/kg per hour). Mesenteric I/R were conducted by a ligation of the mesenteric artery and vein (10 min) and subsequent release of the occlusion. TSA was continuously infused either starting from 10 min before the ischemia or 10 min after reperfusion. Changes in mesenteric microcirculatory variables, including diameter of venule, velocity of red blood cells in venule, leukocyte adhesion, free radicals released from venule, albumin leakage and mast cell degranulation, were observed through an inverted intravital microscope. Meanwhile, the expression of adhesion molecules CD11b/CD18 on neutrophils was evaluated by flow cytometry. Ultrastructural evidence of mesenteric venules damage was assessed after microcirculation observation.
RESULTS: I/R led to multiple responses in mesenteric post-capillary venules, including a significant increase in the adhesion of leukocytes, production of oxygen radicals in the venular wall, albumin efflux and enhanced mast cell degranulation in vivo. All the I/R-induced manifestations were significantly reduced by pre- or post-treatment with TSA, with the exception that the I/R-induced increase in mast cell degranulation was inhibited only by pre-treatment with TSA. Moreover, pre- or post-treatment with TSA significantly attenuated the expression of CD11b/CD18 on neutrophils, reducing the increase in the number of caveolae in the endothelial cells of mesentery post-capillary venules induced by I/R.
CONCLUSION: The results demonstrated that TSA protects from and ameliorates the microcirculation disturbance induced by I/R, which was associated with TSA inhibiting the production of oxygen-free radicals in the venular wall and the expression of CD11b/CD18 on neutrophils. (C) 2010 Baishideng. All rights reserved.
|资助机构||Ministry of Science and Technology of the People&prime ; s Republic of China|
|作者单位||1.Peking Univ, Dept Integrat Chinese & Western Med, Sch Basic Med Sci, Beijing 100191, Peoples R China|
2.Peking Univ, Tasly Microcirculat Res Ctr, Hlth Sci Ctr, Beijing 100191, Peoples R China
3.Tianjin Univ Tradit Chinese Med, Dept Pharmacol, Tianjin 300193, Peoples R China
|Wang, Ming-Xia,Liu, Yu-Ying,Hu, Bai-He,et al. Total salvianolic acid improves ischemia-reperfusion-induced microcirculatory disturbance in rat mesentery[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2010,16(42):5306-5316.|
|APA||Wang, Ming-Xia.,Liu, Yu-Ying.,Hu, Bai-He.,Wei, Xiao-Hong.,Chang, Xin.,...&Han, Jing-Yan.(2010).Total salvianolic acid improves ischemia-reperfusion-induced microcirculatory disturbance in rat mesentery.WORLD JOURNAL OF GASTROENTEROLOGY,16(42),5306-5316.|
|MLA||Wang, Ming-Xia,et al."Total salvianolic acid improves ischemia-reperfusion-induced microcirculatory disturbance in rat mesentery".WORLD JOURNAL OF GASTROENTEROLOGY 16.42(2010):5306-5316.|
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