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学科主题: 临床医学
题名:
Identification of Gene Clusters Associated with Host Adaptation and Antibiotic Resistance in Chinese Staphylococcus aureus Isolates by Microarray-Based Comparative Genomics
作者: Li, Henan1; Zhao, Chunjiang1; Chen, Hongbin1; Zhang, Feifei1; He, Wenqiang1; Wang, Xiaojuan1; Wang, Qi1; Yang, Ruifu2; Zhou, Dongsheng2; Wang, Hui1
刊名: PLOS ONE
发表日期: 2013-01-07
DOI: 10.1371/journal.pone.0053341
卷: 8, 期:1
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: CHEMOTAXIS INHIBITORY PROTEIN ; EVOLUTIONARY GENOMICS ; INSIGHTS ; STRAIN ; EPIDERMIDIS ; VIRULENCE ; EPIDEMIC ; CLONES ; ORIGIN
英文摘要:

A comparative genomic microarray comprising 2,457 genes from two whole genomes of S. aureus was employed for the comparative genome hybridization analysis of 50 strains of divergent clonal lineages, including methicillin-resistant S. aureus (MRSA), methicillin-susceptible S. aureus (MSSA), and swine strains in China. Large-scale validation was confirmed via polymerase chain reaction in 160 representative clinical strains. All of the 50 strains were clustered into seven different complexes by phylogenetic tree analysis. Thirteen gene clusters were specific to different S. aureus clones. Ten gene clusters, including seven known (vSa3, vSa4, vSa alpha, vSa beta, Tn5801, and phage phi Sa3) and three novel (C8, C9, and C10) gene clusters, were specific to human MRSA. Notably, two global regulators, sarH2 and sarH3, at cluster C9 were specific to human MRSA, and plasmid pUB110 at cluster C10 was specific to swine MRSA. Three clusters known to be part of SCCmec, vSa4 or Tn5801, and vSa alpha as well as one novel gene cluster C12 with homology with Tn554 of S. epidermidis were identified as MRSA-specific gene clusters. The replacement of ST239-spa t037 with ST239-spa t030 in Beijing may be a result of its acquisition of vSa4, phage phi Sa1, and phi Sa3. In summary, thirteen critical gene clusters were identified to be contributors to the evolution of host specificity and antibiotic resistance in Chinese S. aureus.

语种: 英语
所属项目编号: 30971571 ; NCET-10-0205 ; 7102130 ; 2012EP001002
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; Beijing Natural Science Foundation ; Key Projects in the National Science &amp ; Technology Pillar Program
WOS记录号: WOS:000313429100056
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63588
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Clin Lab, Beijing 100871, Peoples R China
2.Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing, Peoples R China

Recommended Citation:
Li, Henan,Zhao, Chunjiang,Chen, Hongbin,et al. Identification of Gene Clusters Associated with Host Adaptation and Antibiotic Resistance in Chinese Staphylococcus aureus Isolates by Microarray-Based Comparative Genomics[J]. PLOS ONE,2013,8(1).
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