IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Identification of Gene Clusters Associated with Host Adaptation and Antibiotic Resistance in Chinese Staphylococcus aureus Isolates by Microarray-Based Comparative Genomics
Li, Henan1; Zhao, Chunjiang1; Chen, Hongbin1; Zhang, Feifei1; He, Wenqiang1; Wang, Xiaojuan1; Wang, Qi1; Yang, Ruifu2; Zhou, Dongsheng2; Wang, Hui1
刊名PLOS ONE
2013-01-07
DOI10.1371/journal.pone.0053341
8期:1
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; Beijing Natural Science Foundation ; Key Projects in the National Science &amp ; Technology Pillar Program ; National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; Beijing Natural Science Foundation ; Key Projects in the National Science &amp ; Technology Pillar Program
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]CHEMOTAXIS INHIBITORY PROTEIN ; EVOLUTIONARY GENOMICS ; INSIGHTS ; STRAIN ; EPIDERMIDIS ; VIRULENCE ; EPIDEMIC ; CLONES ; ORIGIN
英文摘要

A comparative genomic microarray comprising 2,457 genes from two whole genomes of S. aureus was employed for the comparative genome hybridization analysis of 50 strains of divergent clonal lineages, including methicillin-resistant S. aureus (MRSA), methicillin-susceptible S. aureus (MSSA), and swine strains in China. Large-scale validation was confirmed via polymerase chain reaction in 160 representative clinical strains. All of the 50 strains were clustered into seven different complexes by phylogenetic tree analysis. Thirteen gene clusters were specific to different S. aureus clones. Ten gene clusters, including seven known (vSa3, vSa4, vSa alpha, vSa beta, Tn5801, and phage phi Sa3) and three novel (C8, C9, and C10) gene clusters, were specific to human MRSA. Notably, two global regulators, sarH2 and sarH3, at cluster C9 were specific to human MRSA, and plasmid pUB110 at cluster C10 was specific to swine MRSA. Three clusters known to be part of SCCmec, vSa4 or Tn5801, and vSa alpha as well as one novel gene cluster C12 with homology with Tn554 of S. epidermidis were identified as MRSA-specific gene clusters. The replacement of ST239-spa t037 with ST239-spa t030 in Beijing may be a result of its acquisition of vSa4, phage phi Sa1, and phi Sa3. In summary, thirteen critical gene clusters were identified to be contributors to the evolution of host specificity and antibiotic resistance in Chinese S. aureus.

语种英语
所属项目编号30971571 ; NCET-10-0205 ; 7102130 ; 2012EP001002
资助者National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; Beijing Natural Science Foundation ; Key Projects in the National Science &amp ; Technology Pillar Program ; National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; Beijing Natural Science Foundation ; Key Projects in the National Science &amp ; Technology Pillar Program
WOS记录号WOS:000313429100056
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63588
专题北京大学第二临床医学院
作者单位1.Peking Univ, Peoples Hosp, Dept Clin Lab, Beijing 100871, Peoples R China
2.Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Li, Henan,Zhao, Chunjiang,Chen, Hongbin,et al. Identification of Gene Clusters Associated with Host Adaptation and Antibiotic Resistance in Chinese Staphylococcus aureus Isolates by Microarray-Based Comparative Genomics[J]. PLOS ONE,2013,8(1).
APA Li, Henan.,Zhao, Chunjiang.,Chen, Hongbin.,Zhang, Feifei.,He, Wenqiang.,...&Wang, Hui.(2013).Identification of Gene Clusters Associated with Host Adaptation and Antibiotic Resistance in Chinese Staphylococcus aureus Isolates by Microarray-Based Comparative Genomics.PLOS ONE,8(1).
MLA Li, Henan,et al."Identification of Gene Clusters Associated with Host Adaptation and Antibiotic Resistance in Chinese Staphylococcus aureus Isolates by Microarray-Based Comparative Genomics".PLOS ONE 8.1(2013).
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