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学科主题: 临床医学
题名:
Calcitonin gene-related peptide in Langerhans cells in psoriatic plaque lesions
作者: He, YL; Ding, GF; Wang, X; Zhu, TJ; Fan, SG
关键词: psoriasis ; Langerhans cell ; calcitonin gene-related peptide
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2000-08-01
卷: 113, 期:8, 页:747-751
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: NERVES
英文摘要:

Objective To study the mechanism of stress exacerbating psoriasis and the involvement effect of neuropeptides in psoriatic pathogenesis, we investigated the expression and secretion of calcitonin gene-related peptide (CGRP) in psoriatic lesions, then identified the target cells of CGRP, the characters of CGRP positive dendrite cells and the source of CGRP in psoriatic plaque lesions.

Methods Specific radioimmunoassay (RIA) and immunohistochemistry staining methods were used to determine CGRP secretive content and the target cells of CGRP in psoriatic plaque lesion tissue of vulgaris psoriasis. Double immunofluorenscence staining was done on psoriatic plaque lesion sections by first using rabbit anti-human CGRP antibody and mouse antihuman CD1a antibody, second using PE-conjugated anti-mouse immunoglobulin and FITC-conjugated anti-rabbit immunoglobulin. Confocal laser microscope showed the psoriatic lesion sections. Then both digoxigenin labelled anti-sense and sense RNA probe of CGRP were synthesized to make sure the source of CGRP on the dendrite cells. The psoriatic lesion sections were studied by in situ hybridization.

Results The content of CGRP in vulgaris psoriatic plaque lesions was higher than that of normal controls (P < 0.01). CGRP was also found on the dermal microvascular endothelial cells and the epidermal dendrite cells in psoriatic plague lesions. Further study showed that CGRP existed on the surface of epidermal CD1a + Langerhans cell in psoriatic plaque lesion. The CGRP mRNA expressed around the nucleus of the Langerhans cells in psoriatic lesion.

Conclusions The pathogenesis of psoriatic plaque lesions was closely related to the overexpression of neuropeptide CGRP. The CGRP contacted with the dermal microvascular endothelial cells and epidermal dendrite cells in psoriatic plaque lesion. The CGRP positive epidermal dendrite cell was CD1a + Langerhans cell. The Langerhans cell itself expressed CGRP mRNA.

语种: 英语
WOS记录号: WOS:000088781300017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63612
Appears in Collections:北京大学第二临床医学院_皮科_期刊论文

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作者单位: 1.Beijing Med Univ, Dept Immunol, Beijing 100083, Peoples R China
2.Beijing Med Univ, Dept Physiol, Beijing 100083, Peoples R China
3.Beijing Med Univ, Peoples Hosp, Dept Dermatol, Beijing 100044, Peoples R China

Recommended Citation:
He, YL,Ding, GF,Wang, X,et al. Calcitonin gene-related peptide in Langerhans cells in psoriatic plaque lesions[J]. CHINESE MEDICAL JOURNAL,2000,113(8):747-751.
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