IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma
Jia, Ling-fei1; Wei, Su-bi2; Gong, Kai3; Gan, Ye-hua4; Yu, Guang-yan1
刊名PLOS ONE
2013-02-22
DOI10.1371/journal.pone.0056634
8期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]CHROMOSOME 11Q13 AMPLIFICATION ; SUPPRESSES TUMORIGENICITY ; HEPATOCELLULAR-CARCINOMA ; GENE AMPLIFICATION ; PROMOTES APOPTOSIS ; BCL-2 EXPRESSION ; POOR-PROGNOSIS ; CYCLIN D1 ; MICRORNA ; CANCER
英文摘要

Background: miR-195 is aberrantly expressed in multiple types of disease. But little is known about the dysregulation of miR-195 in tongue squamous cell carcinoma (TSCC). In this study, we investigated the roles of miR-195 in the development and progression of TSCC.

Methods: Using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), we evaluated miR-195 expression in TSCC samples from 81 patients. Overall survival of these patients was examined using Kaplan-Meier curves with log-rank tests and the Cox proportional hazards model. The expression of two known miR-195 target genes, Cyclin D1 and Bcl-2, was also examined in the TSCC samples by immunohistochemistry. The effects of miR-195 overexpression on cell cycle progression and apoptosis and its effects on the expression of Cyclin D1 and Bcl-2 were examined in transfected TSCC cell lines (SCC-15 and Cal27) using fluorescence-activated cell sorting assays, luciferase reporter assays, and Western blots.

Results: Reduced miR-195 expression was associated with tumor size and the clinical stage of TSCC tumors. Kaplan-Meier survival analysis indicated that the TSCC patients with reduced expression of miR-195 had poor overall survival and in multivariable analyses low levels of miR-195 emerged as an independent prognostic factor for this clinical outcome. Levels of miR-195 expression were inversely correlated with the expression of Cyclin D1 and Bcl-2. Overexpression of miR-195 inhibited cell cycle progression, promoted apoptosis, and reduced Cyclin D1 and Bcl-2 expression in two TSCC cell lines.

Conclusions: miR-195 may have potential applications as a prognostic factor for TSCC patients.

语种英语
WOS记录号WOS:000316658800021
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63613
Collection北京大学口腔医学院
作者单位1.Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Beijing 100871, Peoples R China
2.Tsinghua Univ, Med Syst Biol Res Ctr, Beijing 100084, Peoples R China
3.Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Sch Life Sci, Beijing 100084, Peoples R China
4.Peking Univ, Cent Lab, Sch & Hosp Stomatol, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Jia, Ling-fei,Wei, Su-bi,Gong, Kai,et al. Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma[J]. PLOS ONE,2013,8(2).
APA Jia, Ling-fei,Wei, Su-bi,Gong, Kai,Gan, Ye-hua,&Yu, Guang-yan.(2013).Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma.PLOS ONE,8(2).
MLA Jia, Ling-fei,et al."Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma".PLOS ONE 8.2(2013).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Jia, Ling-fei]'s Articles
[Wei, Su-bi]'s Articles
[Gong, Kai]'s Articles
百度学术
百度学术Similar articles in
[Jia, Ling-fei]'s Articles
[Wei, Su-bi]'s Articles
[Gong, Kai]'s Articles
必应学术
必应学术Similar articles in
[Jia, Ling-fei]'s Articles
[Wei, Su-bi]'s Articles
[Gong, Kai]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.