|Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma|
|Jia, Ling-fei1; Wei, Su-bi2; Gong, Kai3; Gan, Ye-hua4; Yu, Guang-yan1|
|WOS标题词||Science & Technology|
|研究领域[WOS]||Science & Technology - Other Topics|
|关键词[WOS]||CHROMOSOME 11Q13 AMPLIFICATION ; SUPPRESSES TUMORIGENICITY ; HEPATOCELLULAR-CARCINOMA ; GENE AMPLIFICATION ; PROMOTES APOPTOSIS ; BCL-2 EXPRESSION ; POOR-PROGNOSIS ; CYCLIN D1 ; MICRORNA ; CANCER|
Background: miR-195 is aberrantly expressed in multiple types of disease. But little is known about the dysregulation of miR-195 in tongue squamous cell carcinoma (TSCC). In this study, we investigated the roles of miR-195 in the development and progression of TSCC.
Methods: Using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), we evaluated miR-195 expression in TSCC samples from 81 patients. Overall survival of these patients was examined using Kaplan-Meier curves with log-rank tests and the Cox proportional hazards model. The expression of two known miR-195 target genes, Cyclin D1 and Bcl-2, was also examined in the TSCC samples by immunohistochemistry. The effects of miR-195 overexpression on cell cycle progression and apoptosis and its effects on the expression of Cyclin D1 and Bcl-2 were examined in transfected TSCC cell lines (SCC-15 and Cal27) using fluorescence-activated cell sorting assays, luciferase reporter assays, and Western blots.
Results: Reduced miR-195 expression was associated with tumor size and the clinical stage of TSCC tumors. Kaplan-Meier survival analysis indicated that the TSCC patients with reduced expression of miR-195 had poor overall survival and in multivariable analyses low levels of miR-195 emerged as an independent prognostic factor for this clinical outcome. Levels of miR-195 expression were inversely correlated with the expression of Cyclin D1 and Bcl-2. Overexpression of miR-195 inhibited cell cycle progression, promoted apoptosis, and reduced Cyclin D1 and Bcl-2 expression in two TSCC cell lines.
Conclusions: miR-195 may have potential applications as a prognostic factor for TSCC patients.
|作者单位||1.Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Beijing 100871, Peoples R China|
2.Tsinghua Univ, Med Syst Biol Res Ctr, Beijing 100084, Peoples R China
3.Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Sch Life Sci, Beijing 100084, Peoples R China
4.Peking Univ, Cent Lab, Sch & Hosp Stomatol, Beijing 100871, Peoples R China
|Jia, Ling-fei,Wei, Su-bi,Gong, Kai,et al. Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma[J]. PLOS ONE,2013,8(2).|
|APA||Jia, Ling-fei,Wei, Su-bi,Gong, Kai,Gan, Ye-hua,&Yu, Guang-yan.(2013).Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma.PLOS ONE,8(2).|
|MLA||Jia, Ling-fei,et al."Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma".PLOS ONE 8.2(2013).|