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学科主题: 口腔医学
题名:
Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma
作者: Jia, Ling-fei1; Wei, Su-bi2; Gong, Kai3; Gan, Ye-hua4; Yu, Guang-yan1
刊名: PLOS ONE
发表日期: 2013-02-22
DOI: 10.1371/journal.pone.0056634
卷: 8, 期:2
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: CHROMOSOME 11Q13 AMPLIFICATION ; SUPPRESSES TUMORIGENICITY ; HEPATOCELLULAR-CARCINOMA ; GENE AMPLIFICATION ; PROMOTES APOPTOSIS ; BCL-2 EXPRESSION ; POOR-PROGNOSIS ; CYCLIN D1 ; MICRORNA ; CANCER
英文摘要:

Background: miR-195 is aberrantly expressed in multiple types of disease. But little is known about the dysregulation of miR-195 in tongue squamous cell carcinoma (TSCC). In this study, we investigated the roles of miR-195 in the development and progression of TSCC.

Methods: Using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), we evaluated miR-195 expression in TSCC samples from 81 patients. Overall survival of these patients was examined using Kaplan-Meier curves with log-rank tests and the Cox proportional hazards model. The expression of two known miR-195 target genes, Cyclin D1 and Bcl-2, was also examined in the TSCC samples by immunohistochemistry. The effects of miR-195 overexpression on cell cycle progression and apoptosis and its effects on the expression of Cyclin D1 and Bcl-2 were examined in transfected TSCC cell lines (SCC-15 and Cal27) using fluorescence-activated cell sorting assays, luciferase reporter assays, and Western blots.

Results: Reduced miR-195 expression was associated with tumor size and the clinical stage of TSCC tumors. Kaplan-Meier survival analysis indicated that the TSCC patients with reduced expression of miR-195 had poor overall survival and in multivariable analyses low levels of miR-195 emerged as an independent prognostic factor for this clinical outcome. Levels of miR-195 expression were inversely correlated with the expression of Cyclin D1 and Bcl-2. Overexpression of miR-195 inhibited cell cycle progression, promoted apoptosis, and reduced Cyclin D1 and Bcl-2 expression in two TSCC cell lines.

Conclusions: miR-195 may have potential applications as a prognostic factor for TSCC patients.

语种: 英语
WOS记录号: WOS:000316658800021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63613
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Beijing 100871, Peoples R China
2.Tsinghua Univ, Med Syst Biol Res Ctr, Beijing 100084, Peoples R China
3.Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Sch Life Sci, Beijing 100084, Peoples R China
4.Peking Univ, Cent Lab, Sch & Hosp Stomatol, Beijing 100871, Peoples R China

Recommended Citation:
Jia, Ling-fei,Wei, Su-bi,Gong, Kai,et al. Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma[J]. PLOS ONE,2013,8(2).
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