IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma
Jia, Ling-fei1; Wei, Su-bi2; Gong, Kai3; Gan, Ye-hua4; Yu, Guang-yan1
刊名PLOS ONE
2013-02-22
DOI10.1371/journal.pone.0056634
8期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]CHROMOSOME 11Q13 AMPLIFICATION ; SUPPRESSES TUMORIGENICITY ; HEPATOCELLULAR-CARCINOMA ; GENE AMPLIFICATION ; PROMOTES APOPTOSIS ; BCL-2 EXPRESSION ; POOR-PROGNOSIS ; CYCLIN D1 ; MICRORNA ; CANCER
英文摘要

Background: miR-195 is aberrantly expressed in multiple types of disease. But little is known about the dysregulation of miR-195 in tongue squamous cell carcinoma (TSCC). In this study, we investigated the roles of miR-195 in the development and progression of TSCC.

Methods: Using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), we evaluated miR-195 expression in TSCC samples from 81 patients. Overall survival of these patients was examined using Kaplan-Meier curves with log-rank tests and the Cox proportional hazards model. The expression of two known miR-195 target genes, Cyclin D1 and Bcl-2, was also examined in the TSCC samples by immunohistochemistry. The effects of miR-195 overexpression on cell cycle progression and apoptosis and its effects on the expression of Cyclin D1 and Bcl-2 were examined in transfected TSCC cell lines (SCC-15 and Cal27) using fluorescence-activated cell sorting assays, luciferase reporter assays, and Western blots.

Results: Reduced miR-195 expression was associated with tumor size and the clinical stage of TSCC tumors. Kaplan-Meier survival analysis indicated that the TSCC patients with reduced expression of miR-195 had poor overall survival and in multivariable analyses low levels of miR-195 emerged as an independent prognostic factor for this clinical outcome. Levels of miR-195 expression were inversely correlated with the expression of Cyclin D1 and Bcl-2. Overexpression of miR-195 inhibited cell cycle progression, promoted apoptosis, and reduced Cyclin D1 and Bcl-2 expression in two TSCC cell lines.

Conclusions: miR-195 may have potential applications as a prognostic factor for TSCC patients.

语种英语
WOS记录号WOS:000316658800021
引用统计
被引频次:36[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63613
专题北京大学口腔医学院
北京大学口腔医学院_外科
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Beijing 100871, Peoples R China
2.Tsinghua Univ, Med Syst Biol Res Ctr, Beijing 100084, Peoples R China
3.Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Sch Life Sci, Beijing 100084, Peoples R China
4.Peking Univ, Cent Lab, Sch & Hosp Stomatol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Jia, Ling-fei,Wei, Su-bi,Gong, Kai,et al. Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma[J]. PLOS ONE,2013,8(2).
APA Jia, Ling-fei,Wei, Su-bi,Gong, Kai,Gan, Ye-hua,&Yu, Guang-yan.(2013).Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma.PLOS ONE,8(2).
MLA Jia, Ling-fei,et al."Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma".PLOS ONE 8.2(2013).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Jia, Ling-fei]的文章
[Wei, Su-bi]的文章
[Gong, Kai]的文章
百度学术
百度学术中相似的文章
[Jia, Ling-fei]的文章
[Wei, Su-bi]的文章
[Gong, Kai]的文章
必应学术
必应学术中相似的文章
[Jia, Ling-fei]的文章
[Wei, Su-bi]的文章
[Gong, Kai]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。