学科主题 | 口腔医学 |
Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma | |
Jia, Ling-fei1; Wei, Su-bi2; Gong, Kai3; Gan, Ye-hua4; Yu, Guang-yan1 | |
刊名 | PLOS ONE
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2013-02-22 | |
DOI | 10.1371/journal.pone.0056634 |
卷 | 8期:2 |
收录类别 | SCI |
文章类型 | Article |
WOS标题词 | Science & Technology |
类目[WOS] | Multidisciplinary Sciences |
研究领域[WOS] | Science & Technology - Other Topics |
关键词[WOS] | CHROMOSOME 11Q13 AMPLIFICATION ; SUPPRESSES TUMORIGENICITY ; HEPATOCELLULAR-CARCINOMA ; GENE AMPLIFICATION ; PROMOTES APOPTOSIS ; BCL-2 EXPRESSION ; POOR-PROGNOSIS ; CYCLIN D1 ; MICRORNA ; CANCER |
英文摘要 | Background: miR-195 is aberrantly expressed in multiple types of disease. But little is known about the dysregulation of miR-195 in tongue squamous cell carcinoma (TSCC). In this study, we investigated the roles of miR-195 in the development and progression of TSCC. Methods: Using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), we evaluated miR-195 expression in TSCC samples from 81 patients. Overall survival of these patients was examined using Kaplan-Meier curves with log-rank tests and the Cox proportional hazards model. The expression of two known miR-195 target genes, Cyclin D1 and Bcl-2, was also examined in the TSCC samples by immunohistochemistry. The effects of miR-195 overexpression on cell cycle progression and apoptosis and its effects on the expression of Cyclin D1 and Bcl-2 were examined in transfected TSCC cell lines (SCC-15 and Cal27) using fluorescence-activated cell sorting assays, luciferase reporter assays, and Western blots. Results: Reduced miR-195 expression was associated with tumor size and the clinical stage of TSCC tumors. Kaplan-Meier survival analysis indicated that the TSCC patients with reduced expression of miR-195 had poor overall survival and in multivariable analyses low levels of miR-195 emerged as an independent prognostic factor for this clinical outcome. Levels of miR-195 expression were inversely correlated with the expression of Cyclin D1 and Bcl-2. Overexpression of miR-195 inhibited cell cycle progression, promoted apoptosis, and reduced Cyclin D1 and Bcl-2 expression in two TSCC cell lines. Conclusions: miR-195 may have potential applications as a prognostic factor for TSCC patients. |
语种 | 英语 |
WOS记录号 | WOS:000316658800021 |
Citation statistics | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.bjmu.edu.cn/handle/400002259/63613 |
Collection | 北京大学口腔医学院 |
作者单位 | 1.Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Beijing 100871, Peoples R China 2.Tsinghua Univ, Med Syst Biol Res Ctr, Beijing 100084, Peoples R China 3.Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Sch Life Sci, Beijing 100084, Peoples R China 4.Peking Univ, Cent Lab, Sch & Hosp Stomatol, Beijing 100871, Peoples R China |
Recommended Citation GB/T 7714 | Jia, Ling-fei,Wei, Su-bi,Gong, Kai,et al. Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma[J]. PLOS ONE,2013,8(2). |
APA | Jia, Ling-fei,Wei, Su-bi,Gong, Kai,Gan, Ye-hua,&Yu, Guang-yan.(2013).Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma.PLOS ONE,8(2). |
MLA | Jia, Ling-fei,et al."Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma".PLOS ONE 8.2(2013). |
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